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. 1993 Jun;134(2):465–474. doi: 10.1093/genetics/134.2.465

Absence of Strong Heterosis for Life Span and Other Life History Traits in Caenorhabditis Elegans

T E Johnson 1, E W Hutchinson 1
PMCID: PMC1205490  PMID: 8325483

Abstract

We have examined crosses between wild-type strains of Caenorhabditis elegans for heterosis effects on life span and other life history traits. Hermaphrodites of all wild strains had similar life expectancies but males of two strains had shorter life spans than hermaphrodites while males of two other strains lived longer than hermaphrodites. F(1) hermaphrodite progeny showed no heterosis while some heterosis for longer life span was detected in F(1) males. F(1) hybrids of crosses between two widely studied wild-type strains, N2 (var. Bristol) and Berg BO (var. Bergerac), were examined for rate of development, hermaphrodite fertility, and behavior; there was no heterosis for these life history traits. Both controlled variation of temperature and uncontrolled environmental variation affected the length of life of all genotypes. Significant G X E effects on life span were observed in comparisons of N2 and Berg BO hermaphrodites, or N2 hermaphrodites and males, or N2 and a Ts mutant strain (DH26). Nevertheless, within an experiment, environmental variation was minimal and life spans were quite replicable.

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Selected References

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  1. Albertson D. G. Mapping muscle protein genes by in situ hybridization using biotin-labeled probes. EMBO J. 1985 Oct;4(10):2493–2498. doi: 10.1002/j.1460-2075.1985.tb03961.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks A., Johnson T. E. Genetic specification of life span and self-fertility in recombinant-inbred strains of Caenorhabditis elegans. Heredity (Edinb) 1991 Aug;67(Pt 1):19–28. doi: 10.1038/hdy.1991.60. [DOI] [PubMed] [Google Scholar]
  3. Dykhuizen D. E., Hartl D. L. Selection in chemostats. Microbiol Rev. 1983 Jun;47(2):150–168. doi: 10.1128/mr.47.2.150-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edwards M. K., Wood W. B. Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridization. Dev Biol. 1983 Jun;97(2):375–390. doi: 10.1016/0012-1606(83)90094-5. [DOI] [PubMed] [Google Scholar]
  5. Emmons S. W., Klass M. R., Hirsh D. Analysis of the constancy of DNA sequences during development and evolution of the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1333–1337. doi: 10.1073/pnas.76.3.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fire A., Harrison S. W., Dixon D. A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. Gene. 1990 Sep 14;93(2):189–198. doi: 10.1016/0378-1119(90)90224-f. [DOI] [PubMed] [Google Scholar]
  7. Fire A. Integrative transformation of Caenorhabditis elegans. EMBO J. 1986 Oct;5(10):2673–2680. doi: 10.1002/j.1460-2075.1986.tb04550.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodrick C. L. Life-span and the inheritance of longevity of inbred mice. J Gerontol. 1975 May;30(3):257–263. doi: 10.1093/geronj/30.3.257. [DOI] [PubMed] [Google Scholar]
  9. Gould A. B., Clark A. M. X-ray induced mutations causing adult life-shorting in Drosophila melanogaster. Exp Gerontol. 1977;12(3-4):107–112. doi: 10.1016/0531-5565(77)90017-1. [DOI] [PubMed] [Google Scholar]
  10. Greenwald I. lin-12, a nematode homeotic gene, is homologous to a set of mammalian proteins that includes epidermal growth factor. Cell. 1985 Dec;43(3 Pt 2):583–590. doi: 10.1016/0092-8674(85)90230-2. [DOI] [PubMed] [Google Scholar]
  11. Hodgkin J., Horvitz H. R., Brenner S. Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS. Genetics. 1979 Jan;91(1):67–94. doi: 10.1093/genetics/91.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hutchinson E. W., Rose M. R. Quantitative genetics of postponed aging in Drosophila melanogaster. I. Analysis of outbred populations. Genetics. 1991 Apr;127(4):719–727. doi: 10.1093/genetics/127.4.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson T. E. Aging can be genetically dissected into component processes using long-lived lines of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3777–3781. doi: 10.1073/pnas.84.11.3777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson T. E. Increased life-span of age-1 mutants in Caenorhabditis elegans and lower Gompertz rate of aging. Science. 1990 Aug 24;249(4971):908–912. doi: 10.1126/science.2392681. [DOI] [PubMed] [Google Scholar]
  15. Klass M. R. A method for the isolation of longevity mutants in the nematode Caenorhabditis elegans and initial results. Mech Ageing Dev. 1983 Jul-Aug;22(3-4):279–286. doi: 10.1016/0047-6374(83)90082-9. [DOI] [PubMed] [Google Scholar]
  16. Klass M. R. Aging in the nematode Caenorhabditis elegans: major biological and environmental factors influencing life span. Mech Ageing Dev. 1977 Nov-Dec;6(6):413–429. doi: 10.1016/0047-6374(77)90043-4. [DOI] [PubMed] [Google Scholar]
  17. Krause M., Fire A., Harrison S. W., Priess J., Weintraub H. CeMyoD accumulation defines the body wall muscle cell fate during C. elegans embryogenesis. Cell. 1990 Nov 30;63(5):907–919. doi: 10.1016/0092-8674(90)90494-y. [DOI] [PubMed] [Google Scholar]
  18. Lints F. A., Stoll J., Gruwez G., Lints C. V. An attempt to select for increased longevity in Drosophila melanogaster. Gerontology. 1979;25(4):192–204. doi: 10.1159/000212340. [DOI] [PubMed] [Google Scholar]
  19. Moerman D. G., Benian G. M., Waterston R. H. Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2579–2583. doi: 10.1073/pnas.83.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perkins J. M., Jinks J. L. Environmental and genotype-environmental components of variability. 3. Multiple lines and crosses. Heredity (Edinb) 1968 Aug;23(3):339–356. doi: 10.1038/hdy.1968.48. [DOI] [PubMed] [Google Scholar]
  21. Peto R., Pike M. C., Armitage P., Breslow N. E., Cox D. R., Howard S. V., Mantel N., McPherson K., Peto J., Smith P. G. Design and analysis of randomized clinical trials requiring prolonged observation of each patient. II. analysis and examples. Br J Cancer. 1977 Jan;35(1):1–39. doi: 10.1038/bjc.1977.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roberts P. A., Iredale R. B. Can mutagenesis reveal major genes affecting senescence? Exp Gerontol. 1985;20(2):119–121. doi: 10.1016/0531-5565(85)90047-6. [DOI] [PubMed] [Google Scholar]
  23. Ruvkun G., Giusto J. The Caenorhabditis elegans heterochronic gene lin-14 encodes a nuclear protein that forms a temporal developmental switch. Nature. 1989 Mar 23;338(6213):313–319. doi: 10.1038/338313a0. [DOI] [PubMed] [Google Scholar]
  24. Storer J. B. Longevity and gross pathology at death in 22 inbred mouse strains. J Gerontol. 1966 Jul;21(3):404–409. doi: 10.1093/geronj/21.3.404. [DOI] [PubMed] [Google Scholar]

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