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. 1993 Jul;134(3):729–736. doi: 10.1093/genetics/134.3.729

Chromosome Rearrangements Recovered following Transformation of Neurospora Crassa

D D Perkins 1, J A Kinsey 1, D K Asch 1, G D Frederick 1
PMCID: PMC1205511  PMID: 8349106

Abstract

New chromosome rearrangements were found in 10% or more of mitotically stable transformants. This was shown for transformations involving a variety of different markers, vectors and recipient strains. Breakpoints were randomly distributed among the seven linkage groups. Controls using untransformed protoplasts of the same strains contained almost no rearrangements. A study of molecularly characterized Am(+) transformants showed that rearrangements are frequent when multiple ectopic integration events have occurred. In contrast, rearrangements are absent or infrequent when only the resident locus is restored to am(+) by a homologous event. Sequences of the transforming vector were genetically linked to breakpoints in 6 of 10 translocations that were examined using Southern hybridization or colony blots.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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