Skip to main content
Genetics logoLink to Genetics
. 1993 Aug;134(4):1231–1236. doi: 10.1093/genetics/134.4.1231

Fine Physical Mapping of Ph1, a Chromosome Pairing Regulator Gene in Polyploid Wheat

K S Gill 1, B S Gill 1, T R Endo 1, Y Mukai 1
PMCID: PMC1205590  PMID: 8375657

Abstract

The diploid-like chromosome pairing in polyploid wheat is controlled by the Ph1 (pairing homoeologous) gene that is located on chromosome arm 5BL. By using a combination of cytogenetic and molecular techniques, we report the physical location of the Ph1 gene to a submicroscopic chromosome region (Ph1 gene region) that is flanked by the breakpoints of two deletions (5BL-1 and ph1c) and is marked by a DNA probe (XksuS1). The Ph1 gene region is present distal to the breakpoint of deletion 5BL-1 but proximal to the C-band 5BL2.1. Two other DNA probes (Xpsr128 and Xksu75) flank the region-Xpsr128 being proximal and Xksu75 being distal. The estimated size of the region is less than 3 Mb. The chromosome region around the Ph1 gene is high in recombination as the genetic distance of the region between 5BL-1 breakpoint and C-band 5BL2.1 (not resolved by the microscope) is at least 9.3 cM.

Full Text

The Full Text of this article is available as a PDF (3.6 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedbrook J. R., Jones J., O'Dell M., Thompson R. D., Flavell R. B. A molecular description of telometic heterochromatin in secale species. Cell. 1980 Feb;19(2):545–560. doi: 10.1016/0092-8674(80)90529-2. [DOI] [PubMed] [Google Scholar]
  2. Gill B. S., Kimber G. Giemsa C-banding and the evolution of wheat. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4086–4090. doi: 10.1073/pnas.71.10.4086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gill K. S., Gill B. S. A DNA fragment mapped within the submicroscopic deletion of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics. 1991 Sep;129(1):257–259. doi: 10.1093/genetics/129.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES