Abstract
Fifty-seven Sinhalese patients were investigated for the presence of Helicobacter pylori by PCR. A prevalence of 70.1%, with 47.5% positive for cagA, was demonstrated. The most common vacA allele was s1am1. There was no significant association between either the s1 allele or the cagA allele and severe gastroduodenal disease. There was an association between the s1 allele and the cagA locus.
Helicobacter pylori is one of the most common causes of chronic bacterial infections in humans and is important in the pathogenesis of gastrointestinal disease, such as duodenal ulcer, gastric ulcer, gastric cancer, and lymphoma (1, 2).
Only three studies have reported on the prevalence of H. pylori in Sri Lanka, using either culture or a rapid urease test (RUT). In one study using the RUT, a prevalence of 46% was recorded in an adult symptomatic population (5), while in a study using culture and the RUT, only 12% of a symptomatic population aged 18 to 80 years were recorded as colonized (3). More recently, only 2.9% of 67 patients with functional dyspepsia were positive by the RUT (4). In India, a prevalence of 80% has been reported.
Because the H. pylori strains prevalent in Sri Lanka have not previously been reported upon, the objectives of this study were to determine their prevalence by using PCR, as it is sensitive (7), gives an indication of current infection, and yields additional information regarding the type of strain colonizing the individual.
Fifty-seven dyspeptic Sinhalese patients were enrolled; 41 were males and 16 were females, with an age range of 4 to 74 years (mean, 44 years). Two gastric biopsy samples were taken from the antrum for histology and DNA studies. The DNA was extracted by using a PuregeneD-5500A kit (Flowgen, Ashby de la Zouch, United Kingdom). PCRs for the vacA, cagA, 16S rRNA, and glmM genes were carried out with the primers noted below, using prealiquoted PCR Mastermix in thermotubes (AB Gene, Epsom, Surrey, United Kingdom). The vacA “s” region was amplified with VA1-F (5′ATGGAAATACAACAACACAC3′) and VA1-R (5′CTGCTTGAATGCGCCAAAC3′), which generate a 259-bp amplicon for the s1 allele and a 286-bp amplicon for the s2 allele. The vacA “m” region was amplified with HPMG-F (5′CACAGCCACTTTCAATAACGA3′) and HPMG-R (5′CGTCAAAATAATTCCAAGGG3′), which generate a 401-bp amplicon for the m1 allele and a 476-bp amplicon for the m2 allele. The cycle parameters were 95°C for 2 min (1 cycle) followed by 40 cycles of 95°C for 1 min, 50°C for 1 min, and 74°C for 1 min with a final extension of 74°C for 5 min (10).
Variation within the s1 allele was determined by using the primers SS1-F (5′GTCAGCATCACACCGCAAC3′), SS3-F (5′AGCGCCATACCGCAAGAG3′), SS2-F (5′GCTAACACGCCAAATGATCC3′), and VA1-R (5′CTGCTTGAATGCGCCAAAC3′), which generate a 190-bp amplicon for the s1a allele (SS1F and VA1R), a 187-bp amplicon for the s1b allele (SS3F and VA1R), and a 199-bp amplicon for the s2 allele. The cycle parameters were 95°C for 5 min (1 cycle) followed by 35 cycles of 95°C for 1 min, 52°C for 1 min, and 72°C for 1 min (8). The s1c allele was confirmed by using the primers VA1F and VA1XR using the above cycle parameters, followed by hybridization with a digoxigenin-labeled s1c-specific probe (Dig-GGGCTTATTGGTCTAGCATCATCAC; MWG Biotech, Milton Keynes, United Kingdom) (10). The bound probe was detected with a nucleic acid detection kit (Boehringer GmbH, Mannheim, Germany).
The presence of the cagA gene was demonstrated by using the primers cagA-F (5′TTGACCAACAACCACAAACGAAG3′) and cagA-R(5′CTTCCCTTAATTGCGAGATTCC3′) (11) for 95°C for 2 min (1 cycle) followed by 40 cycles of 95°C for 1 min, 50°C for 1 min, and 74°C for 1 min with a final extension of 74°C for 5 min, which generates an amplicon of 183 bp. The Helicobacter 16S rRNA gene was detected by using the primers JW21-F (5′GCGACCTGCTGGAACATTAC3′) and JW22-R (5′CGTTAGCTGCATTGGAGA3′) (6) with 35 cycles of 93°C for 1 min, 55°C for 1 min, and 72°C for 1 min followed by 72°C for 10 min, which amplifies a 139-bp amplicon. The glmM gene was detected by using the primers F5′AAGCTTTTAGGGGTGTTAGGGGTTT3′ and R5′AAGCTTACTTTCTAACACTAACGC3′ with 35 cycles of 93°C for 1 min, 55°C for 1 min, and 72°C for 1 min (7), which amplifies a 249-bp amplicon.
Forty-six of 57 (80.7%) of these patients had only gastritis, and Helicobacter-like organisms were found in 3 of them; 5 of the 57 (8.7%) had both gastritis and a duodenal ulcer, 2 (3.5%) had gastritis and a gastric ulcer, 1 (1.7%) had gastritis and a gastric carcinoma, and only 3 (5.3%) had normal histology. A total of 40 (70.1%) biopsies were positive for H. pylori by 16S rRNA, glmM, and vacA gene amplification. Of these 40 positive specimens, 19 (47.5%) were also positive for cagA, and colonization by cagA-positive isolates did not relate to the presence of severe gastroduodenal disease (P < 0.05). There was also no correlation between colonization by organisms carrying the s1 allele and severe gastroduodenal disease (P < 0.05). The most common allele type was s1am1, followed by s1bm1. Although they were positive by other primers, nine samples could not be fully subtyped using these primers. In six of these nine, the “m” region could not be typed but the “s” region showed 4s1a and 2s1b, both associated with gastritis, and in three, the “s” region could not be typed further than s1 but all were m1. Two biopsy samples demonstrated multiple infection, with more than one subtype of the “m” region present. Of 35 isolates that were vacA s1 (including those that could not be fully subtyped in the signal sequence), 19 (44%) were cagA positive. Of those that could not be fully subtyped, three of four that were s1, one of two that were s1b, and two of three that could be typed only as s1 were cagA positive. The relationship between the possession of the s1 allele and the cagA locus was statistically significant (P = 0.05).
We have shown that the prevalence of H. pylori in a dyspeptic Sinhalese population is 75.4% by using PCR. The low prevalence in previous studies may reflect low sensitivity of the methods. The predominant vacA allele is the s1am1 type similar to that found in Northern Europe, but both the s1b and s1c allele types were also represented. This may reflect the multiethnic population and historical invasions of Sri Lanka by the English, Portuguese, and Dutch. These data indicate that 45% of the strains were cagA positive, and as in the Far East (9), there was no correlation between either vacA or cagA and severe gastroduodenal disease. There was, however, an association between cagA and the s1 vacA allele, as has been shown in other studies.
Acknowledgments
We thank the Government of Sri Lanka for financial support to N. Fernando.
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