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. 1993 Sep;135(1):117–125. doi: 10.1093/genetics/135.1.117

Mutations in the Protein Phosphatase 1 Gene at 87b Can Differentially Affect Suppression of Position-Effect Variegation and Mitosis in Drosophila Melanogaster

K Baksa 1, H Morawietz 1, V Dombradi 1, M Axton 1, H Taubert 1, G Szabo 1, I Torok 1, A Udvardy 1, H Gyurkovics 1, B Szoor 1, D Glover 1, G Reuter 1, J Gausz 1
PMCID: PMC1205611  PMID: 8224813

Abstract

The suppressor of position effect variegation (PEV) locus Su-var(3)6 maps to 87B5-10. The breakpoints of deficiencies that define this interval have been placed on a 250-kb molecular map of the region. The locus is allelic to the ck19 complementation group previously shown to encode a type 1 serine-threonine protein phosphatase (PP1) catalytic subunit. When introduced into flies by P element-mediated transformation, a 5.8-kb genomic fragment carrying this gene overcomes the suppressor phenotype of Su-var(3)6(01) and recessive lethality of all mutations of the locus. Four of the mutant alleles at the locus show a broad correlation between high levels of suppression of PEV, a high frequency of aberrant mitosis and low PP1 activity in larval extracts. However, some alleles with low PP1 activity show weak suppression of PEV with a high frequency of abnormal mitosis, whereas others show strong suppression of PEV with normal mitosis. The basis for these disparate phenotypes is discussed.

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Selected References

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