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. 1994 Jan;136(1):173–182. doi: 10.1093/genetics/136.1.173

Forked Proteins Are Components of Fiber Bundles Present in Developing Bristles of Drosophila Melanogaster

N S Petersen 1, D H Lankenau 1, H K Mitchell 1, P Young 1, V G Corces 1
PMCID: PMC1205769  PMID: 8138155

Abstract

The forked (f) gene of Drosophila melanogaster encodes six different transcripts 6.4, 5.6, 5.4, 2.5, 1.9, and 1.1 kb long. These transcripts arise by the use of alternative promoters. A polyclonal antibody raised against a domain common to all of the forked-encoded products has been used to identify forked proteins on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and in Drosophila pupal tissues. The antibody stains fiber bundles present in bristle cells for about 15 hr during normal pupal development. Electron microscopy shows that these fibers are present from 40 to 53 hr in bristles of wild-type flies but are absent in the null f(36a) mutant. The forked protein(s) thus appear to be an essential part of the bristle fibers. The phenotype of the f(36a) mutation can be rescued by a 13-kb fragment of the forked locus containing the coding regions for the 2.5, 1.9, and 1.1-kb transcripts, suggesting that the proteins encoded by the three large forked RNAs are dispensable during bristle development. Increasing the copy number of a P[w(+),f(+)] construct containing the 13-kb fragment induces a hypermorphic bristle phenotype whose severity correlates with the number of copies of P[w(+),f(+)] present. These results indicate that alterations in the ratios among the forked proteins, or between forked products and other components of the fiber, result in abnormal assembly of the fibrillar cytoplasmic structures necessary for bristle morphogenesis.

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Selected References

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  1. Appel L. F., Prout M., Abu-Shumays R., Hammonds A., Garbe J. C., Fristrom D., Fristrom J. The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4937–4941. doi: 10.1073/pnas.90.11.4937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burke D., Gasdaska P., Hartwell L. Dominant effects of tubulin overexpression in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Mar;9(3):1049–1059. doi: 10.1128/mcb.9.3.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buzin C. H., Petersen N. S. A comparison of the multiple Drosophila heat shock proteins in cell lines and larval salivary glands by two-dimensional gel electrophoresis. J Mol Biol. 1982 Jun 25;158(2):181–201. doi: 10.1016/0022-2836(82)90428-4. [DOI] [PubMed] [Google Scholar]
  4. Green M. M. A FURTHER ANALYSIS OF THE FORKED LOCUS IN DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1956 Feb;42(2):73–77. doi: 10.1073/pnas.42.2.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoover K. K., Chien A. J., Corces V. G. Effects of transposable elements on the expression of the forked gene of Drosophila melanogaster. Genetics. 1993 Oct;135(2):507–526. doi: 10.1093/genetics/135.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  7. Mitchell H. K., Petersen N. S. Epithelial differentiation in Drosophila pupae. Dev Genet. 1989;10(1):42–52. doi: 10.1002/dvg.1020100107. [DOI] [PubMed] [Google Scholar]
  8. Mitchell H. K., Roach J., Petersen N. S. The morphogenesis of cell hairs on Drosophila wings. Dev Biol. 1983 Feb;95(2):387–398. doi: 10.1016/0012-1606(83)90040-4. [DOI] [PubMed] [Google Scholar]
  9. Overton J. The fine structure of developing bristles in wild type and mutant Drosophila melanogaster. J Morphol. 1967 Aug;122(4):367–379. doi: 10.1002/jmor.1051220406. [DOI] [PubMed] [Google Scholar]
  10. Parkhurst S. M., Corces V. G. Forked, gypsys, and suppressors in Drosophila. Cell. 1985 Jun;41(2):429–437. doi: 10.1016/s0092-8674(85)80016-7. [DOI] [PubMed] [Google Scholar]
  11. Petersen N. S., Young P. Effects of heat shock on protein processing and turnover in developing Drosophila wings. Dev Genet. 1989;10(1):11–15. doi: 10.1002/dvg.1020100103. [DOI] [PubMed] [Google Scholar]
  12. Pirrotta V., Steller H., Bozzetti M. P. Multiple upstream regulatory elements control the expression of the Drosophila white gene. EMBO J. 1985 Dec 16;4(13A):3501–3508. doi: 10.1002/j.1460-2075.1985.tb04109.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ribbert D. Relation of puffing to bristle and footpad differentiation in Calliphora and Sarcophaga. Results Probl Cell Differ. 1972;4:153–179. doi: 10.1007/978-3-540-37164-9_6. [DOI] [PubMed] [Google Scholar]
  14. Spindler K. R., Rosser D. S., Berk A. J. Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. J Virol. 1984 Jan;49(1):132–141. doi: 10.1128/jvi.49.1.132-141.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zipursky S. L., Venkatesh T. R., Teplow D. B., Benzer S. Neuronal development in the Drosophila retina: monoclonal antibodies as molecular probes. Cell. 1984 Jan;36(1):15–26. doi: 10.1016/0092-8674(84)90069-2. [DOI] [PubMed] [Google Scholar]

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