Abstract
The yeast pleiotropic (multiple drug) resistance gene PDR5 encodes a product with homology to a large number of membrane transport proteins including the mammalian multiple drug resistance family. In this study, we identified four genes on chromosome II that affect the steady-state level of PDR5 transcript in addition to a previously identified positive regulator, PDR1. The genes in question are PDR3, PDR4, PDR7 and PDR9. We also analyzed the interaction between PDR5 and YAP1. YAP1 encodes a positive regulator with a leucine zipper motif that causes pleiotropic drug resistance when overproduced. YAP1-mediated pleiotropic drug resistance is not dependent on the presence of PDR5 and must act through other genes.
Full Text
The Full Text of this article is available as a PDF (5.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balzi E., Chen W., Ulaszewski S., Capieaux E., Goffeau A. The multidrug resistance gene PDR1 from Saccharomyces cerevisiae. J Biol Chem. 1987 Dec 15;262(35):16871–16879. [PubMed] [Google Scholar]
- Balzi E., Goffeau A. Multiple or pleiotropic drug resistance in yeast. Biochim Biophys Acta. 1991 Mar 4;1073(2):241–252. doi: 10.1016/0304-4165(91)90128-4. [DOI] [PubMed] [Google Scholar]
- Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moye-Rowley W. S., Harshman K. D., Parker C. S. Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins. Genes Dev. 1989 Mar;3(3):283–292. doi: 10.1101/gad.3.3.283. [DOI] [PubMed] [Google Scholar]
- Perkins D. D. Biochemical Mutants in the Smut Fungus Ustilago Maydis. Genetics. 1949 Sep;34(5):607–626. doi: 10.1093/genetics/34.5.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Preston R. A., Garman J. D., Daniels L. B., Jones E. W. Map positions of pet9, pep1 and pdr4 with respect to CEN2. Yeast. 1991 Nov;7(8):857–858. doi: 10.1002/yea.320070811. [DOI] [PubMed] [Google Scholar]
- Ruttkay-Nedecký B., Obernauerová M., Subík J. High-level resistance to cycloheximide resulting from an interaction of the mutated pdr3 and cyh genes in yeast. Curr Genet. 1992 Oct;22(4):337–339. doi: 10.1007/BF00317932. [DOI] [PubMed] [Google Scholar]
- Subik J., Ulaszewski S., Goffeau A. Genetic mapping of nuclear mucidin resistance mutations in Saccharomyces cerevisiae. A new pdr locus on chromosome II. Curr Genet. 1986;10(9):665–670. doi: 10.1007/BF00410914. [DOI] [PubMed] [Google Scholar]