Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1994 Mar;136(3):1013–1023. doi: 10.1093/genetics/136.3.1013

Drosophila P Element Transposase Induces Male Recombination Additively and without a Requirement for P Element Excision or Insertion

M McCarron 1, A Duttaroy 1, G Doughty 1, A Chovnick 1
PMCID: PMC1205859  PMID: 8005411

Abstract

P element dysgenesis-associated male recombination in Drosophila was examined with a selective system focused upon a section of the third chromosome divided into eight recombination segments. Tests compared crossing over in the presence of none, one and two doses of P(δ2-3)(99B), a non-mobile transposase source, in the absence of a mobilizable P element target in the genome. In the presence of the P transposase source, and without a P element target, significant male recombination occurred in genomic regions physically separated from the P(δ2-3) site. Using two doses of P(δ2-3) without a P element target, the male recombination rate doubled, and 90% of the crossovers occurred in the pericentric region. The distribution of recombination events, in the absence of P element targets approximates that seen in studies of radiation induced mitotic crossing over and the metaphase chromosome map. Another experiment examined the effects of one dose of P(δ2-3) on a genome with a single P element target, P(lArB)(87C9), in the third recombination segment. Crossovers increased 58-fold in the immediate region of the P element target.

Full Text

The Full Text of this article is available as a PDF (2.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg R., Engels W. R., Kreber R. A. Site-specific X-chromosome rearrangements from hybrid dysgenesis in Drosophila melanogaster. Science. 1980 Oct;210(4468):427–429. doi: 10.1126/science.6776625. [DOI] [PubMed] [Google Scholar]
  2. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  3. Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
  4. Duttaroy A., McCarron M., Sitaraman K., Doughty G., Chovnick A. The relationship between P elements and male recombination in Drosophila melanogaster. Genetics. 1990 Feb;124(2):317–329. doi: 10.1093/genetics/124.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engels W. R., Preston C. R. Formation of chromosome rearrangements by P factors in Drosophila. Genetics. 1984 Aug;107(4):657–678. doi: 10.1093/genetics/107.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Engels W. R., Preston C. R. Identifying P factors in Drosophila by means of chromosome breakage hotspots. Cell. 1981 Nov;26(3 Pt 1):421–428. doi: 10.1016/0092-8674(81)90211-7. [DOI] [PubMed] [Google Scholar]
  7. Henikoff S. Position Effects and Variegation Enhancers in an Autosomal Region of DROSOPHILA MELANOGASTER. Genetics. 1979 Sep;93(1):105–115. doi: 10.1093/genetics/93.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hiraizumi Y., Slatko B., Langley C., Nill A. Recombination in Drosophila melanogaster male. Genetics. 1973 Mar;73(3):439–444. doi: 10.1093/genetics/73.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hiraizumi Y. Spontaneous recombination in Drosophila melanogaster males. Proc Natl Acad Sci U S A. 1971 Feb;68(2):268–270. doi: 10.1073/pnas.68.2.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isackson D. R., Johnson T. K., Denell R. E. Hybrid dysgenesis in Drosophila: the mechanism of T-007-induced male recombination. Mol Gen Genet. 1981;184(3):539–543. doi: 10.1007/BF00352536. [DOI] [PubMed] [Google Scholar]
  11. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  12. Kidwell M. G., Kidwell J. F. Selection for male recombination in Drosophila melanogaster. Genetics. 1976 Oct;84(2):333–351. doi: 10.1093/genetics/84.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kidwell M. G., Novy J. B. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: Sterility Resulting from Gonadal Dysgenesis in the P-M System. Genetics. 1979 Aug;92(4):1127–1140. doi: 10.1093/genetics/92.4.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kidwell M. G. Reciprocal differences in female recombination associated with hybrid dysgenesis in Drosophila melanogaster. Genet Res. 1977 Aug;30(1):77–88. doi: 10.1017/s001667230001747x. [DOI] [PubMed] [Google Scholar]
  15. McCarron M. Y., Duttaroy A., Doughty G. A., Chovnick A. P-element transposase induces male recombination in Drosophila melanogaster. Genet Res. 1989 Oct;54(2):137–141. doi: 10.1017/s0016672300028500. [DOI] [PubMed] [Google Scholar]
  16. Morata G., Ripoll P. Minutes: mutants of drosophila autonomously affecting cell division rate. Dev Biol. 1975 Feb;42(2):211–221. doi: 10.1016/0012-1606(75)90330-9. [DOI] [PubMed] [Google Scholar]
  17. Reuter G., Giarre M., Farah J., Gausz J., Spierer A., Spierer P. Dependence of position-effect variegation in Drosophila on dose of a gene encoding an unusual zinc-finger protein. Nature. 1990 Mar 15;344(6263):219–223. doi: 10.1038/344219a0. [DOI] [PubMed] [Google Scholar]
  18. Rio D. C. Molecular mechanisms regulating Drosophila P element transposition. Annu Rev Genet. 1990;24:543–578. doi: 10.1146/annurev.ge.24.120190.002551. [DOI] [PubMed] [Google Scholar]
  19. Rio D. C. Regulation of Drosophila P element transposition. Trends Genet. 1991 Sep;7(9):282–287. doi: 10.1016/0168-9525(91)90309-E. [DOI] [PubMed] [Google Scholar]
  20. Robertson H. M., Preston C. R., Phillis R. W., Johnson-Schlitz D. M., Benz W. K., Engels W. R. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics. 1988 Mar;118(3):461–470. doi: 10.1093/genetics/118.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rushlow C. A., Bender W., Chovnick A. Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster. Genetics. 1984 Nov;108(3):603–615. doi: 10.1093/genetics/108.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Slatko B. E. Parameters of Male and Female Recombination Influenced by the T-007 Second Chromosome in DROSOPHILA MELANOGASTER. Genetics. 1978 Oct;90(2):257–276. doi: 10.1093/genetics/90.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sved J. A., Blackman L. M., Gilchrist A. S., Engels W. R. High levels of recombination induced by homologous P elements in Drosophila melanogaster. Mol Gen Genet. 1991 Mar;225(3):443–447. doi: 10.1007/BF00261685. [DOI] [PubMed] [Google Scholar]
  24. Sved J. A., Eggleston W. B., Engels W. R. Germ-line and somatic recombination induced by in vitro modified P elements in Drosophila melanogaster. Genetics. 1990 Feb;124(2):331–337. doi: 10.1093/genetics/124.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Voelker R. A. The genetics and cytology of a mutator factor in Drosophila melanogaster. Mutat Res. 1974 Mar;22(3):265–276. doi: 10.1016/0027-5107(74)90027-x. [DOI] [PubMed] [Google Scholar]
  26. Wilson C., Pearson R. K., Bellen H. J., O'Kane C. J., Grossniklaus U., Gehring W. J. P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. Genes Dev. 1989 Sep;3(9):1301–1313. doi: 10.1101/gad.3.9.1301. [DOI] [PubMed] [Google Scholar]
  27. Yannopoulos G., Stamatis N., Zacharopoulou A., Pelecanos M. Site-specific breaks induced by the male recombination factor 23.5 MRF in Drosophila melanogaster. Mutat Res. 1983 Mar;108(1-3):185–202. doi: 10.1016/0027-5107(83)90120-3. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES