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. 1994 Apr;136(4):1319–1324. doi: 10.1093/genetics/136.4.1319

The Role of Macronuclear DNA Sequences in the Permanent Rescue of a Non-Mendelian Mutation in Paramecium Tetraurelia

Y You 1, J Scott 1, J Forney 1
PMCID: PMC1205912  PMID: 8013908

Abstract

The Paramecium tetraurelia mutant called d48 has a complete copy of the A surface protein gene in its micronuclei, but lacks the A gene in the macronucleus. Previous experiments have shown that microinjection of a plasmid containing the entire A gene or a large portion of the gene into the macronucleus of d48 rescued the cell line after formation of a new macronucleus (autogamy). Here we show that several different regions of the A gene can rescue d48, but 100% of the activity cannot be localized to a single, defined region. Inversion of a sequence contained within an A gene plasmid had no measurable effect on rescue efficiency and co-injection of two different plasmids results in enhancement of rescue activity despite the non-contiguous form of the DNA sequences. Both these results suggest that no specific product (RNA or protein) with defined end points is made from the rescuing fragment. A unique restriction site was created in the A gene and used to demonstrate that the injected DNA does not serve as a direct template for the synthesis of the new macronuclear DNA. Models to explain the action of the injected DNA are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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