In the published article, there was an error in Figure 1 as published. The two figures above and below in Figures 1K-L , related to SOX2 staining, are part of a series of photographs aimed at demonstrating that GB explants—small tissue fragments approximately 300 µm in size—retain the original cytoarchitecture of the tissue from which they are derived (surgery tissue). The upper figure represents an image of the surgery tissue, while the lower figure shows the corresponding small explant (GB-EXP) derived from it. The SOX2 staining was specifically performed to add further evidence of the preserved representation of tumor components within the GB explant, a conclusion also supported by other images in the series (GFAP; CD105,CD33).
Figure 1.
GB-EXPs cultures (A) Experimental design. (B) A surgery tumor sample. (C) GB-EXP embedded in matrigel. (D) H&E staining of a surgery GB tissue. (E) Live/dead staining of a GB-EXP at 2 weeks after culturing. (F, G) Ki67 immunostaining of GB-EXPs in suspension at 0 week and 2 weeks. (H) Ki67 mRNA expression analysis of 13 GB case-derived GB-EXPs at 0 and 2 weeks. Graph represents mean ± s.d. of triplicated measures. (I) Representative brightfield and area size images of a GB-EXP in matrigel at 0 week, 1 week and 2 weeks. (J) Size analysis 20 GB case-derived GB-EXPs at 0, 1 and 2 weeks. (K, L) Immunofluorescence assays of surgery GB tissue (K) and GB-EXPs (L). GFAP (astrocytes), IBA1 (microglia), SOX2 (stem cells), CD105 (endothelial cells) and CD3 (lymphocytes). WTA, Whole Transcriptome Analysis; WEA, Whole Exome Analysis; FC, Flow Cytometry; H&E, Hematoxylin, and Eosin; MGMT, MGMT promoter methylation analysis.
We acknowledge an error of duplication in the figures related to SOX2, as the upper image is, in fact, a photo of the same explant but with a slightly shifted area. We deeply regret this misplacement and are providing the correct image of the tumor stained with SOX2.The corrected Figure 1 and its legend appear below.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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