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. 1994 Jul;137(3):759–769. doi: 10.1093/genetics/137.3.759

Isolation and Characterization of Paramecium Mutants Defective in Their Response to Magnesium

R R Preston 1, C Kung 1
PMCID: PMC1206036  PMID: 8088522

Abstract

Four mutant strains of Paramecium tetraurelia with a reduced ability to respond behaviorally to Mg(2+) have been isolated. Voltage-clamp analyses showed that their Mg(2+) insensitivity is associated with a reduced Ca(2+) -dependent Mg(2+) current. The four mutants, which have been dubbed ``eccentric,'' result from recessive mutations in two unlinked loci, xntA and xntB. Further analysis of xntA(1) showed it to be unlinked to any of the behavioral mutants of P. tetraurelia described previously, but it is allelic to d4-521, a ``K(+)-resistant'' strain, and d4-596, a ``Ba(2+)-shy'' mutant. The varied pleiotropic effects of xntA(1), which include increased resistance to Ni(2+) and Zn(2+) poisoning, suggest that the locus encodes a central regulator of cell function in Paramecium.

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Selected References

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  1. Chang S. Y., Kung C. Selection and analysis of a mutant Paramecium tetraurelia lacking behavioural response to tetraethylammonium. Genet Res. 1976 Apr;27(2):97–107. doi: 10.1017/s0016672300016311. [DOI] [PubMed] [Google Scholar]
  2. Dalemans W., Barbry P., Champigny G., Jallat S., Dott K., Dreyer D., Crystal R. G., Pavirani A., Lecocq J. P., Lazdunski M. Altered chloride ion channel kinetics associated with the delta F508 cystic fibrosis mutation. Nature. 1991 Dec 19;354(6354):526–528. doi: 10.1038/354526a0. [DOI] [PubMed] [Google Scholar]
  3. Evans T. C., Hennessey T., Nelson D. L. Electrophysiological evidence suggests a defective Ca2+ control mechanism in a new Paramecium mutant. J Membr Biol. 1987;98(3):275–283. doi: 10.1007/BF01871189. [DOI] [PubMed] [Google Scholar]
  4. Evans T. C., Nelson D. L. New mutants of Paramecium tetraurelia defective in a calcium control mechanism: genetic and behavioral characterizations. Genetics. 1989 Mar;121(3):491–500. doi: 10.1093/genetics/121.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Flatman P. W. Mechanisms of magnesium transport. Annu Rev Physiol. 1991;53:259–271. doi: 10.1146/annurev.ph.53.030191.001355. [DOI] [PubMed] [Google Scholar]
  6. Grubbs R. D., Maguire M. E. Magnesium as a regulatory cation: criteria and evaluation. Magnesium. 1987;6(3):113–127. [PubMed] [Google Scholar]
  7. Hennessey T. M., Kung C. A calcium-dependent potassium current is increased by a single-gene mutation in Paramecium. J Membr Biol. 1987;98(2):145–155. doi: 10.1007/BF01872127. [DOI] [PubMed] [Google Scholar]
  8. Hinrichsen R. D., Amberger E., Saimi Y., Burgess-Cassler A., Kung C. Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia. Genetics. 1985 Nov;111(3):433–445. doi: 10.1093/genetics/111.3.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hinrichsen R. D., Saimi Y. A mutation that alters properties of the calcium channel in Paramecium tetraurelia. J Physiol. 1984 Jun;351:397–410. doi: 10.1113/jphysiol.1984.sp015252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kink J. A., Maley M. E., Preston R. R., Ling K. Y., Wallen-Friedman M. A., Saimi Y., Kung C. Mutations in paramecium calmodulin indicate functional differences between the C-terminal and N-terminal lobes in vivo. Cell. 1990 Jul 13;62(1):165–174. doi: 10.1016/0092-8674(90)90250-i. [DOI] [PubMed] [Google Scholar]
  11. Kung C. Genic mutants with altered system of excitation in Paramecium aurelia. II. Mutagenesis, screening and genetic analysis of the mutants. Genetics. 1971 Sep;69(1):29–45. doi: 10.1093/genetics/69.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lefort-Tran M., Aufderheide K., Pouphile M., Rossignol M., Beisson J. Control of exocytotic processes: cytological and physiological studies of trichocyst mutants in Paramecium tetraurelia. J Cell Biol. 1981 Feb;88(2):301–311. doi: 10.1083/jcb.88.2.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oertel D., Schein S. J., Kung C. Separation of membrane currents using a Paramecium mutant. Nature. 1977 Jul 14;268(5616):120–124. doi: 10.1038/268120a0. [DOI] [PubMed] [Google Scholar]
  14. Papazian D. M., Schwarz T. L., Tempel B. L., Jan Y. N., Jan L. Y. Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila. Science. 1987 Aug 14;237(4816):749–753. doi: 10.1126/science.2441470. [DOI] [PubMed] [Google Scholar]
  15. Preston R. R., Kink J. A., Hinrichsen R. D., Saimi Y., Kung C. Calmodulin mutants and Ca2(+)-dependent channels in Paramecium. Annu Rev Physiol. 1991;53:309–319. doi: 10.1146/annurev.ph.53.030191.001521. [DOI] [PubMed] [Google Scholar]
  16. Preston R. R., Saimi Y., Amberger E., Kung C. Interactions between mutants with defects in two Ca2(+)-dependent K+ currents of Paramecium tetraurelia. J Membr Biol. 1990 Apr;115(1):61–69. doi: 10.1007/BF01869106. [DOI] [PubMed] [Google Scholar]
  17. Preston R. R., Saimi Y., Kung C. Calcium current activated upon hyperpolarization of Paramecium tetraurelia. J Gen Physiol. 1992 Aug;100(2):233–251. doi: 10.1085/jgp.100.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Preston R. R., Wallen-Friedman M. A., Saimi Y., Kung C. Calmodulin defects cause the loss of Ca2(+)-dependent K+ currents in two pantophobiac mutants of Paramecium tetraurelia. J Membr Biol. 1990 Apr;115(1):51–60. doi: 10.1007/BF01869105. [DOI] [PubMed] [Google Scholar]
  19. Rojas C. V., Wang J. Z., Schwartz L. S., Hoffman E. P., Powell B. R., Brown R. H., Jr A Met-to-Val mutation in the skeletal muscle Na+ channel alpha-subunit in hyperkalaemic periodic paralysis. Nature. 1991 Dec 5;354(6352):387–389. doi: 10.1038/354387a0. [DOI] [PubMed] [Google Scholar]
  20. Romani A., Scarpa A. Regulation of cell magnesium. Arch Biochem Biophys. 1992 Oct;298(1):1–12. doi: 10.1016/0003-9861(92)90086-c. [DOI] [PubMed] [Google Scholar]
  21. Saimi Y., Hinrichsen R. D., Forte M., Kung C. Mutant analysis shows that the Ca2+-induced K+ current shuts off one type of excitation in Paramecium. Proc Natl Acad Sci U S A. 1983 Aug;80(16):5112–5116. doi: 10.1073/pnas.80.16.5112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Saimi Y., Kung C. Behavioral genetics of Paramecium. Annu Rev Genet. 1987;21:47–65. doi: 10.1146/annurev.ge.21.120187.000403. [DOI] [PubMed] [Google Scholar]
  23. Saimi Y., Ling K. Y. Calmodulin activation of calcium-dependent sodium channels in excised membrane patches of Paramecium. Science. 1990 Sep 21;249(4975):1441–1444. doi: 10.1126/science.2169650. [DOI] [PubMed] [Google Scholar]
  24. Satow Y., Chang S. Y., Kung C. Membrane excitability: made temperature-dependent by mutations. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2703–2706. doi: 10.1073/pnas.71.7.2703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Satow Y., Kung C. Membrane currents of pawn mutants of the pwA group in Paramecium tetraurelia. J Exp Biol. 1980 Feb;84:57–71. doi: 10.1242/jeb.84.1.57. [DOI] [PubMed] [Google Scholar]
  26. Shusterman C. L., Thiede E. W., Kung C. K-resistant mutants and "adaptation" in Paramecium. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5645–5649. doi: 10.1073/pnas.75.11.5645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. White R. E., Hartzell H. C. Magnesium ions in cardiac function. Regulator of ion channels and second messengers. Biochem Pharmacol. 1989 Mar 15;38(6):859–867. doi: 10.1016/0006-2952(89)90272-4. [DOI] [PubMed] [Google Scholar]
  28. van Houten J., Chang S. Y., Kung C. Genetic analyses of "paranoiac" mutants of Paramecium tetraurelia. Genetics. 1977 May;86(1):113–120. doi: 10.1093/genetics/86.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]

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