Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1994 Sep;138(1):7–10.

Presence of Different O Antigen Forms in Three Isolates of One Clone of Escherichia Coli

D Liu 1, P R Reeves 1
PMCID: PMC1206139  PMID: 7528154

Abstract

Escherichia coli strains ECOR2, ECOR3 and K-12 are very closely related in genotype as indicated by multilocus enzyme electrophoresis. We show that they have very different rfb regions indicating that recombination has occurred in this region, and we suggest that it may be associated with niche adaptation.

Full Text

The Full Text of this article is available as a PDF (1.5 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., Väisanen-Rhen V., Korhonen T. K., Stuart S., Orskov F. Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infect Immun. 1986 Jan;51(1):268–276. doi: 10.1128/iai.51.1.268-276.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bastin D. A., Romana L. K., Reeves P. R. Molecular cloning and expression in Escherichia coli K-12 of the rfb gene cluster determining the O antigen of an E. coli O111 strain. Mol Microbiol. 1991 Sep;5(9):2223–2231. doi: 10.1111/j.1365-2958.1991.tb02152.x. [DOI] [PubMed] [Google Scholar]
  3. Bouffard G., Ostell J., Rudd K. E. GeneScape: a relational database of Escherichia coli genomic map data for Macintosh computers. Comput Appl Biosci. 1992 Dec;8(6):563–567. doi: 10.1093/bioinformatics/8.6.563. [DOI] [PubMed] [Google Scholar]
  4. Brown P. K., Romana L. K., Reeves P. R. Cloning of the rfb gene cluster of a group C2 Salmonella strain: comparison with the rfb regions of groups B and D. Mol Microbiol. 1991 Aug;5(8):1873–1881. doi: 10.1111/j.1365-2958.1991.tb00811.x. [DOI] [PubMed] [Google Scholar]
  5. Jacobs W. R., Barrett J. F., Clark-Curtiss J. E., Curtiss R., 3rd In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries. Infect Immun. 1986 Apr;52(1):101–109. doi: 10.1128/iai.52.1.101-109.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jiang X. M., Neal B., Santiago F., Lee S. J., Romana L. K., Reeves P. R. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol Microbiol. 1991 Mar;5(3):695–713. doi: 10.1111/j.1365-2958.1991.tb00741.x. [DOI] [PubMed] [Google Scholar]
  7. Kanehisa M. I. Los Alamos sequence analysis package for nucleic acids and proteins. Nucleic Acids Res. 1982 Jan 11;10(1):183–196. doi: 10.1093/nar/10.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kapur V., White D. G., Wilson R. A., Whittam T. S. Outer membrane protein patterns mark clones of Escherichia coli O2 and O78 strains that cause avian septicemia. Infect Immun. 1992 Apr;60(4):1687–1691. doi: 10.1128/iai.60.4.1687-1691.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  10. Lee S. J., Romana L. K., Reeves P. R. Sequence and structural analysis of the rfb (O antigen) gene cluster from a group C1 Salmonella enterica strain. J Gen Microbiol. 1992 Sep;138(9):1843–1855. doi: 10.1099/00221287-138-9-1843. [DOI] [PubMed] [Google Scholar]
  11. Liu D., Reeves P. R. Escherichia coli K12 regains its O antigen. Microbiology. 1994 Jan;140(Pt 1):49–57. doi: 10.1099/13500872-140-1-49. [DOI] [PubMed] [Google Scholar]
  12. Marolda C. L., Valvano M. A. Identification, expression, and DNA sequence of the GDP-mannose biosynthesis genes encoded by the O7 rfb gene cluster of strain VW187 (Escherichia coli O7:K1). J Bacteriol. 1993 Jan;175(1):148–158. doi: 10.1128/jb.175.1.148-158.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nasoff M. S., Baker H. V., 2nd, Wolf R. E., Jr DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase. Gene. 1984 Mar;27(3):253–264. doi: 10.1016/0378-1119(84)90070-2. [DOI] [PubMed] [Google Scholar]
  14. Neuhard J., Thomassen E. Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene. J Bacteriol. 1976 May;126(2):999–1001. doi: 10.1128/jb.126.2.999-1001.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reeves P. R. Variation in O-antigens, niche-specific selection and bacterial populations. FEMS Microbiol Lett. 1992 Dec 15;100(1-3):509–516. doi: 10.1111/j.1574-6968.1992.tb14085.x. [DOI] [PubMed] [Google Scholar]
  16. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tartof K. D., Hobbs C. A. New cloning vectors and techniques for easy and rapid restriction mapping. Gene. 1988 Jul 30;67(2):169–182. doi: 10.1016/0378-1119(88)90394-0. [DOI] [PubMed] [Google Scholar]
  18. Wang L., Romana L. K., Reeves P. R. Molecular analysis of a Salmonella enterica group E1 rfb gene cluster: O antigen and the genetic basis of the major polymorphism. Genetics. 1992 Mar;130(3):429–443. doi: 10.1093/genetics/130.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES