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. 1994 Oct;138(2):289–296. doi: 10.1093/genetics/138.2.289

Isolation of the Mating-Type Genes of the Phytopathogenic Fungus Magnaporthe Grisea Using Genomic Subtraction

S Kang 1, F G Chumley 1, B Valent 1
PMCID: PMC1206148  PMID: 7828813

Abstract

Using genomic subtraction, we isolated the mating-type genes (Mat1-1 and Mat1-2) of the rice blast fungus, Magnaporthe grisea. Transformation of M. grisea strains of one mating type with a linearized cosmid clone carrying the opposite mating-type gene resulted in many ``dual maters,'' strains that contain both mating-type genes and successfully mate with both Mat1-1 and Mat1-2 testers. Dual maters differed in the frequency of production of perithecia in pure culture. Ascospores isolated from these homothallic crosses were either Mat1-1 or Mat1-2, but there were no dual maters. Most conidia from dual maters also had one or the other of the mating-type genes, but not both. Thus, dual maters appear to lose one of the mating-type genes during vegetative growth. The incidence of self-mating in dual maters appears to depend on the co-occurrence of strains with each mating type in vegetative cultures. In rare transformants, the incoming sequences had replaced the resident mating-type gene. Nearly isogenic pairs produced from three M. grisea laboratory strains were mated to investigate their fertility. One transformant with switched mating type appears to have a mutation that impairs the development of asci when its mating partner has a similar genetic background. The M. grisea Mat1-1 and Mat1-2 genes are idiomorphs approximately 2.5 and 3.5 kb in length, respectively.

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Selected References

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  1. Debuchy R., Coppin E. The mating types of Podospora anserina: functional analysis and sequence of the fertilization domains. Mol Gen Genet. 1992 May;233(1-2):113–121. doi: 10.1007/BF00587568. [DOI] [PubMed] [Google Scholar]
  2. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  3. Hamer J. E., Farrall L., Orbach M. J., Valent B., Chumley F. G. Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9981–9985. doi: 10.1073/pnas.86.24.9981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  5. Ong T. M., Serres F. J. Mutation induction by difunctional alkylating agents in Neurospora crassa. Genetics. 1975 Jul;(3):475–482. doi: 10.1093/genetics/80.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Orbach M. J., Vollrath D., Davis R. W., Yanofsky C. An electrophoretic karyotype of Neurospora crassa. Mol Cell Biol. 1988 Apr;8(4):1469–1473. doi: 10.1128/mcb.8.4.1469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Picard M., Debuchy R., Coppin E. Cloning the mating types of the heterothallic fungus Podospora anserina: developmental features of haploid transformants carrying both mating types. Genetics. 1991 Jul;128(3):539–547. doi: 10.1093/genetics/128.3.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Staben C., Yanofsky C. Neurospora crassa a mating-type region. Proc Natl Acad Sci U S A. 1990 Jul;87(13):4917–4921. doi: 10.1073/pnas.87.13.4917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sweigard J. A., Chumley F. G., Valent B. Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. Mol Gen Genet. 1992 Mar;232(2):174–182. doi: 10.1007/BF00279994. [DOI] [PubMed] [Google Scholar]
  10. Sweigard J. A., Chumley F. G., Valent B. Disruption of a Magnaporthe grisea cutinase gene. Mol Gen Genet. 1992 Mar;232(2):183–190. [PubMed] [Google Scholar]
  11. Talbot N. J., Salch Y. P., Ma M., Hamer J. E. Karyotypic Variation within Clonal Lineages of the Rice Blast Fungus, Magnaporthe grisea. Appl Environ Microbiol. 1993 Feb;59(2):585–593. doi: 10.1128/aem.59.2.585-593.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Valent B., Farrall L., Chumley F. G. Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics. 1991 Jan;127(1):87–101. doi: 10.1093/genetics/127.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wieland I., Bolger G., Asouline G., Wigler M. A method for difference cloning: gene amplification following subtractive hybridization. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2720–2724. doi: 10.1073/pnas.87.7.2720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zachar Z., Bingham P. M. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell. 1982 Sep;30(2):529–541. doi: 10.1016/0092-8674(82)90250-1. [DOI] [PubMed] [Google Scholar]

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