Abstract
Differences in the composition of the population of mtDNAs between green plants and calli of tobacco were detected by DNA filter hybridization analysis. The altered composition of the population of mtDNAs observed in calli returned to the composition typical of green plants during the process of regeneration. Quantitative assays revealed that the changes were associated with the differentiation and dedifferentiation of cells since the extent of the change in composition depended on the degree of differentiation of a population of cells. The sequence that accumulated in dedifferentiated cells was shown to be a product of recombination mediated by a 9-nucleotide repeated element, one of which is located at the 5' region of atp6. Although the recombinant sequence was not detected by a hybridization procedure in green plants, its presence was identified by a more sensitive polymerase chain reaction method. The recombination event was shown to result in a deletion that prevents reverse recombination. Therefore, the reversion from the altered composition to the normal state of the population of mtDNAs during regeneration is explained not by recombination but by the preferential amplification of subgenomic mtDNA molecules.
Full Text
The Full Text of this article is available as a PDF (5.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Almasan A., Mishra N. C. Recombination by sequence repeats with formation of suppressive or residual mitochondrial DNA in Neurospora. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7684–7688. doi: 10.1073/pnas.88.17.7684. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartmann C., Récipon H., Jubier M. F., Valon C., Delcher-Besin E., Henry Y., De Buyser J., Lejeune B., Rode A. Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat. Curr Genet. 1994 May;25(5):456–464. doi: 10.1007/BF00351786. [DOI] [PubMed] [Google Scholar]
- King M. P., Attardi G. Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science. 1989 Oct 27;246(4929):500–503. doi: 10.1126/science.2814477. [DOI] [PubMed] [Google Scholar]
- Lonsdale D. M., Hodge T. P., Fauron C. M. The physical map and organisation of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res. 1984 Dec 21;12(24):9249–9261. doi: 10.1093/nar/12.24.9249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martínez-Zapater J. M., Gil P., Capel J., Somerville C. R. Mutations at the Arabidopsis CHM locus promote rearrangements of the mitochondrial genome. Plant Cell. 1992 Aug;4(8):889–899. doi: 10.1105/tpc.4.8.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shoffner J. M., Lott M. T., Voljavec A. S., Soueidan S. A., Costigan D. A., Wallace D. C. Spontaneous Kearns-Sayre/chronic external ophthalmoplegia plus syndrome associated with a mitochondrial DNA deletion: a slip-replication model and metabolic therapy. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7952–7956. doi: 10.1073/pnas.86.20.7952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Small I. D., Isaac P. G., Leaver C. J. Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J. 1987 Apr;6(4):865–869. doi: 10.1002/j.1460-2075.1987.tb04832.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vitart V., De Paepe R., Mathieu C., Chétrit P., Vedel F. Amplification of substoichiometric recombinant mitochondrial DNA sequences in a nuclear, male sterile mutant regenerated from protoplast culture in Nicotiana sylvestris. Mol Gen Genet. 1992 May;233(1-2):193–200. doi: 10.1007/BF00587579. [DOI] [PubMed] [Google Scholar]
- Wallace D. C. Mitochondrial genetics: a paradigm for aging and degenerative diseases? Science. 1992 May 1;256(5057):628–632. doi: 10.1126/science.1533953. [DOI] [PubMed] [Google Scholar]
- de Zamaroczy M., Faugeron-Fonty G., Bernardi G. Excision sequences in the mitochondrial genome of yeast. Gene. 1983 Mar;21(3):193–202. doi: 10.1016/0378-1119(83)90002-1. [DOI] [PubMed] [Google Scholar]