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. 1994 Dec;138(4):1207–1238. doi: 10.1093/genetics/138.4.1207

A Genetic Linkage Map of the Mouse Using Restriction Landmark Genomic Scanning (Rlgs)

Y Hayashizaki 1, S Hirotsune 1, Y Okazaki 1, H Shibata 1, A Akasako 1, M Muramatsu 1, J Kawai 1, T Hirasawa 1, S Watanabe 1, T Shiroishi 1, K Moriwaki 1, B A Taylor 1, Y Matsuda 1, R W Elliott 1, K F Manly 1, V M Chapman 1
PMCID: PMC1206259  PMID: 7896102

Abstract

We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled restriction fragments of genomic DNA that are separated by using high resolution, two-dimensional gel electrophoresis identifying as many as two thousand landmark loci in a single gel. Variation for several hundred of these loci has been identified between laboratory strains and between these strains and Mus spretus. The segregation of more than 1100 RLGS loci has been analyxed in recombinant inbred (RI) strains and in two separate interspecific genetic crosses. Genetic maps have been derived that link 1045 RLGS loci to reference loci on all of the autosomes and the X chromosome of the mouse genome. The RLGS method can be applied to genome analysis in many different organisms to identify genomic loci because it used end-labeling of restriction landmarks rather than probe hybridization. Different combinations of restriction enzymes yield different sets of RLGS loci providing expanded power for genetic mapping.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beier D. R., Dushkin H., Sussman D. J. Mapping genes in the mouse using single-strand conformation polymorphism analysis of recombinant inbred strains and interspecific crosses. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9102–9106. doi: 10.1073/pnas.89.19.9102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Copeland N. G., Jenkins N. A., Gilbert D. J., Eppig J. T., Maltais L. J., Miller J. C., Dietrich W. F., Weaver A., Lincoln S. E., Steen R. G. A genetic linkage map of the mouse: current applications and future prospects. Science. 1993 Oct 1;262(5130):57–66. doi: 10.1126/science.8211130. [DOI] [PubMed] [Google Scholar]
  3. Dietrich W., Katz H., Lincoln S. E., Shin H. S., Friedman J., Dracopoli N. C., Lander E. S. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics. 1992 Jun;131(2):423–447. doi: 10.1093/genetics/131.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hayashizaki Y., Hirotsune S., Hatada I., Tamatsukuri S., Miyamoto C., Furuichi Y., Mukai T. A new method for constructing NotI linking and boundary libraries using a restriction trapper. Genomics. 1992 Nov;14(3):733–739. doi: 10.1016/s0888-7543(05)80176-4. [DOI] [PubMed] [Google Scholar]
  5. Hayashizaki Y., Hirotsune S., Okazaki Y., Hatada I., Shibata H., Kawai J., Hirose K., Watanabe S., Fushiki S., Wada S. Restriction landmark genomic scanning method and its various applications. Electrophoresis. 1993 Apr;14(4):251–258. doi: 10.1002/elps.1150140145. [DOI] [PubMed] [Google Scholar]
  6. Hearne C. M., McAleer M. A., Love J. M., Aitman T. J., Cornall R. J., Ghosh S., Knight A. M., Prins J. B., Todd J. A. Additional microsatellite markers for mouse genome mapping. Mamm Genome. 1991;1(4):273–282. doi: 10.1007/BF00352339. [DOI] [PubMed] [Google Scholar]
  7. Hirotsune S., Hatada I., Komatsubara H., Nagai H., Kuma K., Kobayakawa K., Kawara T., Nakagawara A., Fujii K., Mukai T. New approach for detection of amplification in cancer DNA using restriction landmark genomic scanning. Cancer Res. 1992 Jul 1;52(13):3642–3647. [PubMed] [Google Scholar]
  8. Hirotsune S., Shibata H., Okazaki Y., Sugino H., Imoto H., Sasaki N., Hirose K., Okuizumi H., Muramatsu M., Plass C. Molecular cloning of polymorphic markers on RLGS gel using the spot target cloning method. Biochem Biophys Res Commun. 1993 Aug 16;194(3):1406–1412. doi: 10.1006/bbrc.1993.1981. [DOI] [PubMed] [Google Scholar]
  9. Kawai J., Hirotsune S., Hirose K., Fushiki S., Watanabe S., Hayashizaki Y. Methylation profiles of genomic DNA of mouse developmental brain detected by restriction landmark genomic scanning (RLGS) method. Nucleic Acids Res. 1993 Dec 11;21(24):5604–5608. doi: 10.1093/nar/21.24.5604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Le Roy H., Simon-Chazottes D., Montagutelli X., Guénet J. L. A set of anonymous DNA clones as markers for mouse gene mapping. Mamm Genome. 1992;3(4):244–246. doi: 10.1007/BF00355727. [DOI] [PubMed] [Google Scholar]
  11. Manly K. F., Elliott R. W. RI Manager, a microcomputer program for analysis of data from recombinant inbred strains. Mamm Genome. 1991;1(2):123–126. doi: 10.1007/BF02443789. [DOI] [PubMed] [Google Scholar]
  12. Matsuda Y., Chapman V. M. In situ analysis of centromeric satellite DNA segregating in Mus species crosses. Mamm Genome. 1991;1(2):71–77. doi: 10.1007/BF02443781. [DOI] [PubMed] [Google Scholar]
  13. Matsuda Y., Manly K. F., Chapman V. M. In situ analysis of centromere segregation in C57BL/6 x Mus spretus interspecific backcrosses. Mamm Genome. 1993 Sep;4(9):475–480. doi: 10.1007/BF00364780. [DOI] [PubMed] [Google Scholar]

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