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. 1995 Feb;139(2):1091–1097. doi: 10.1093/genetics/139.2.1091

Multilocus Analysis for Gene-Centromere Mapping Using First Polar Bodies and Secondary Oocytes

Y Da 1, V L Jarrell 1, T Wang 1, R L Fernando 1, M B Wheeler 1, H A Lewin 1
PMCID: PMC1206359  PMID: 7713411

Abstract

Polar body and oocyte typing is a new technique for gene-centromere mapping and for generating female linkage maps. A maximum likelihood approach is presented for ordering multiple markers relative to the centromere and for estimating recombination frequencies between markers and between the centromere and marker loci. Three marker-centromere orders are possible for each pair of markers: two orders when the centromere flanks the two markers and one order when the centromere is flanked by the two markers. For each possible order, the likelihood was expressed as a function of recombination frequencies for two adjacent intervals. LOD score for recombination frequency between markers or between the centromere and a marker locus was derived based on the likelihood for each gene-centromere order. The methods developed herein provide a general solution to the problem of multilocus genecentromere mapping that involves all theoretical crossover possibilities, including four-strand double crossovers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boehnke M., Arnheim N., Li H., Collins F. S. Fine-structure genetic mapping of human chromosomes using the polymerase chain reaction on single sperm: experimental design considerations. Am J Hum Genet. 1989 Jul;45(1):21–32. [PMC free article] [PubMed] [Google Scholar]
  2. Chakravarti A., Slaugenhaupt S. A. Methods for studying recombination on chromosomes that undergo nondisjunction. Genomics. 1987 Sep;1(1):35–42. doi: 10.1016/0888-7543(87)90102-9. [DOI] [PubMed] [Google Scholar]
  3. Cui X. F., Li H. H., Goradia T. M., Lange K., Kazazian H. H., Jr, Galas D., Arnheim N. Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9389–9393. doi: 10.1073/pnas.86.23.9389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cui X., Gerwin J., Navidi W., Li H., Kuehn M., Arnheim N. Gene-centromere linkage mapping by PCR analysis of individual oocytes. Genomics. 1992 Jul;13(3):713–717. doi: 10.1016/0888-7543(92)90146-j. [DOI] [PubMed] [Google Scholar]
  5. Côté G. B., Edwards J. H. Centrometric linkage in autosomal trisomies. Ann Hum Genet. 1975 Jul;39(1):51–59. doi: 10.1111/j.1469-1809.1975.tb00107.x. [DOI] [PubMed] [Google Scholar]
  6. Dunn L. C., Bennett D. Sex differences in recombination of linked genes in animals. Genet Res. 1967 Apr;9(2):211–220. doi: 10.1017/s0016672300010491. [DOI] [PubMed] [Google Scholar]
  7. Eppig J. T., Eicher E. M. Application of the ovarian teratoma mapping method in the mouse. Genetics. 1983 Apr;103(4):797–812. doi: 10.1093/genetics/103.4.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goradia T. M., Lange K. Multilocus ordering strategies based on sperm typing. Ann Hum Genet. 1990 Jan;54(Pt 1):49–77. doi: 10.1111/j.1469-1809.1990.tb00360.x. [DOI] [PubMed] [Google Scholar]
  9. Lander E. S., Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989 Jan;121(1):185–199. doi: 10.1093/genetics/121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matise T. C., Perlin M., Chakravarti A. Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage map. Nat Genet. 1994 Apr;6(4):384–390. doi: 10.1038/ng0494-384. [DOI] [PubMed] [Google Scholar]
  11. Ott J., Linder D., McCaw B. K., Lovrien E. W., Hecht F. Estimating distances from the centromere by means of benign ovarian teratomas in man. Ann Hum Genet. 1976 Nov;40(2):191–196. doi: 10.1111/j.1469-1809.1976.tb00179.x. [DOI] [PubMed] [Google Scholar]
  12. Snow R. Maximum likelihood estimation of linkage and interference from tetrad data. Genetics. 1979 May;92(1):231–245. doi: 10.1093/genetics/92.1.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van Eijk M. J., Russ I., Lewin H. A. Order of bovine DRB3, DYA, and PRL determined by sperm typing. Mamm Genome. 1993;4(2):113–118. doi: 10.1007/BF00290436. [DOI] [PubMed] [Google Scholar]

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