Abstract
1. An organism, identified as Micrococcus sp., was isolated by elective culture on aconate; it also grew on itaconate. 2. Washed suspensions of the aconate-grown organism readily oxidized intermediates of the tricarboxylic acid cycle, aconate and succinic semialdehyde, but not itaconate. Itaconate-grown cells oxidized tricarboxylic acid-cycle intermediates, succinic semialdehyde and itaconate, but not aconate. Succinate-grown cells oxidized neither itaconate nor aconate. 3. Extracts of aconate-grown cells catalysed the formation of succinic semialdehyde and carbon dioxide, in equimolar amounts, from aconate. In the presence of NAD or NADP, succinic semialdehyde was oxidized to succinate with concomitant reduction of the coenzyme. 4. Extracts of itaconate-grown cells catalysed the formation of pyruvate and acetyl-CoA from itaconyl-CoA. 5. Key enzymes involved in the formation of succinate from aconate, and of pyruvate and acetyl-CoA from itaconate, were distinct and inducible: their formation preceded growth on the appropriate substrate.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADLER J., WANG S. F., LARDY H. A. The metabolism of itaconic acid by liver mitochondria. J Biol Chem. 1957 Dec;229(2):865–879. [PubMed] [Google Scholar]
- Brightman V., Martin W. R. PATHWAY FOR THE DISSIMILATION OF ITACONIC AND MESACONIC ACIDS. J Bacteriol. 1961 Sep;82(3):376–382. doi: 10.1128/jb.82.3.376-382.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper R. A., Kornberg H. L. The utilization of itaconate by Pseudomonas sp. Biochem J. 1964 Apr;91(1):82–91. doi: 10.1042/bj0910082. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EL HAWARY M. F. S., THOMPSON R. H. S. Separation and estimation of blood keto acids by paper chromatography. Biochem J. 1953 Feb;53(3):340–347. doi: 10.1042/bj0530340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KORNBERG H. L. Selective utilization of metabolic routes by Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:257–260. doi: 10.1101/sqb.1961.026.01.032. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- WANG S. F., ADLER J., LARDY H. A. The pathway of itaconate metabolism by liver mitochondria. J Biol Chem. 1961 Jan;236:26–30. [PubMed] [Google Scholar]