Abstract
1. The calf-thymus histone group F2a has been separated into two subfractions by stepwise precipitation with acetone from acid solution. 2. Carboxymethyl-cellulose and dextran-gel column chromatography and a method involving dialysis against ethanol have also given the subfractions, but the acetone–hydrochloric acid method has proved to be the most practicable. 3. The two subfractions differ significantly in their amino acid composition and in the pattern of peptides obtained by tryptic digestion. Both fractions have a very low content of N-terminal amino acids and contain acetyl groups.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- CRUFT H. J., HINDLEY J., MAURITZEN C. M., STEDMAN E. Amino-acid composition of the six histones of calf thymocytes. Nature. 1957 Nov 23;180(4595):1107–1109. doi: 10.1038/1801107a0. [DOI] [PubMed] [Google Scholar]
- JOHNS E. W., PHILLIPS D. M., SIMSON P., BUTLER J. A. Improved fractionations of arginine-rich histones from calf thymus. Biochem J. 1960 Dec;77:631–636. doi: 10.1042/bj0770631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOHNS E. W., PHILLIPS D. M., SIMSON P., BUTLER J. A. The electrophoresis of histones and histone fractions on starch gel. Biochem J. 1961 Jul;80:189–193. doi: 10.1042/bj0800189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johns E. W. Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem J. 1964 Jul;92(1):55–59. doi: 10.1042/bj0920055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVY A. L. A paper chromatographic method for the quantitative estimation of amino-acids. Nature. 1954 Jul 17;174(4420):126–127. doi: 10.1038/174126a0. [DOI] [PubMed] [Google Scholar]
- PHILLIPS D. M. A micromethod for the determination of N- and O-acetyl groups. Biochem J. 1963 Mar;86:397–401. doi: 10.1042/bj0860397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PHILLIPS D. M., JOHNS E. W. A study of the proteinase content and the chromatography of thymus histones. Biochem J. 1959 Jul;72:538–544. doi: 10.1042/bj0720538. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PHILLIPS D. M., SIMSON P. Identification of some peptides from an arginine-rich histone and their bearing on the structure of deoxyribonucleohistone. Biochem J. 1962 Feb;82:236–241. doi: 10.1042/bj0820236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PHILLIPS D. M. The N-terminal groups of calf-thymus histones. Biochem J. 1958 Jan;68(1):35–40. doi: 10.1042/bj0680035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PHILLIPS D. M. The presence of acetyl groups of histones. Biochem J. 1963 May;87:258–263. doi: 10.1042/bj0870258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F. The free amino groups of insulin. Biochem J. 1945;39(5):507–515. doi: 10.1042/bj0390507. [DOI] [PMC free article] [PubMed] [Google Scholar]