Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 Jan;94(1):166–170. doi: 10.1042/bj0940166

Amino acid composition and C-terminal residues of algal biliproteins

M A Raftery 1, C Ó Heocha 1
PMCID: PMC1206423  PMID: 14342224

Abstract

1. R-phycoerythrin from Ceramium rubrum and C-phycocyanin from Nostoc muscorum have been obtained in purified form by fractional crystallization, followed by chromatography and gel-filtration. 2. The amino acid composition of both chromoproteins was determined; 85% of the total weight of both was recovered as amino acids. 3. Alanine was identified as the only C-terminal amino acid of R-phycoerythrin, each molecule of which contained about 12 terminal groups. 4. Serine was identified as the only C-terminal group of C-phycocyanin.

Full text

PDF
166

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRESTFIELD A. M., MOORE S., STEIN W. H. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963 Feb;238:622–627. [PubMed] [Google Scholar]
  2. Eriksson-Quensel I. B. The molecular weights of phycoerythrin and phycocyan. I. Biochem J. 1938 Mar;32(3):585–589. doi: 10.1042/bj0320585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FUJIWARA T. Studies on chromoproteins in Japanese nori, Porphyra tenera. V. On the sugar components of phycoerythrin. J Biochem. 1961 May;49:361–367. doi: 10.1093/oxfordjournals.jbchem.a127310. [DOI] [PubMed] [Google Scholar]
  4. FUNATSU G., TSUGITA A., FRAENKEL-CONRAT H. STUDIES ON THE AMINO ACID SEQUENCE OF TOBACCO MOSAIC VIRUS PROTEIN. V. AMINO ACID SEQUENCES OF TWO PEPTIDES FROM TRYPTIC DIGESTS AND LOCATION OF AMIDE GROUP. Arch Biochem Biophys. 1964 Apr;105:25–41. doi: 10.1016/0003-9861(64)90232-2. [DOI] [PubMed] [Google Scholar]
  5. HAXO F., O'HEOCHA C., NORRIS P. Comparative studies of chromatographically separated phycoerythrins and phycocyanins. Arch Biochem Biophys. 1955 Jan;54(1):162–173. doi: 10.1016/0003-9861(55)90019-9. [DOI] [PubMed] [Google Scholar]
  6. KIMMEL J. R., SMITH E. L. The amino acid composition of crystalline pumpkin seed globulin, edestin, C-phycocyanin and R-phycoerythrin. Bull Soc Chim Biol (Paris) 1958;40(12):2049–2065. [PubMed] [Google Scholar]
  7. LEVY A. L. A paper chromatographic method for the quantitative estimation of amino-acids. Nature. 1954 Jul 17;174(4420):126–127. doi: 10.1038/174126a0. [DOI] [PubMed] [Google Scholar]
  8. PORATH J. Gel filtration of proteins, peptides and amino acids. Biochim Biophys Acta. 1960 Apr 8;39:193–207. doi: 10.1016/0006-3002(60)90153-0. [DOI] [PubMed] [Google Scholar]
  9. SCHRAM E., MOORE S., BIGWOOD E. J. Chromatographic determination of cystine as cysteic acid. Biochem J. 1954 May;57(1):33–37. doi: 10.1042/bj0570033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanger F. The free amino groups of insulin. Biochem J. 1945;39(5):507–515. doi: 10.1042/bj0390507. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES