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. 1995 Mar;139(3):1273–1291. doi: 10.1093/genetics/139.3.1273

High Resolution Mapping of Genetic Factors Affecting Abdominal Bristle Number in Drosophila Melanogaster

A D Long 1, S L Mullaney 1, L A Reid 1, J D Fry 1, C H Langley 1, TFC Mackay 1
PMCID: PMC1206456  PMID: 7768438

Abstract

Factors responsible for selection response for abdominal bristle number and correlated responses in sternopleural bristle number were mapped to the X and third chromosome of Drosophila melanogaster. Lines divergent for high and low abdominal bristle number were created by 25 generations of artificial selection from a large base population, with an intensity of 25 individuals of each sex selected from 100 individuals of each sex scored per generation. Isogenic chromosome substitution lines in which the high (H) X or third chromosome were placed in an isogenic low (L) background were derived from the selection lines and from the 93 recombinant isogenic (RI) HL X and 67 RI chromosome 3 lines constructed from them. Highly polymorphic neutral r00 transposable elements were hybridized in situ to the polytene chromosomes of the RI lines to create a set of cytogenetic markers. These techniques yielded a dense map with an average spacing of 4 cM between informative markers. Factors affecting bristle number, and relative viability of the chromosome 3 RI lines, were mapped using a multiple regression interval mapping approach, conditioning on all markers >/=10 cM from the tested interval. Two factors with large effects on abdominal bristle number were mapped on the X chromosome and five factors on the third chromosome. One factor with a large effect on sternopleural bristle number was mapped to the X and two were mapped to the third chromosome; all factors with sternopleural effects corresponded to those with effects on abdominal bristle number. Two of the chromosome 3 factors with large effects on abdominal bristle number were also associated with reduced viability. Significant sex-specific effects and epistatic interactions between mapped factors of the same order of magnitude as the additive effects were observed. All factors mapped to the approximate positions of likely candidate loci (ASC, bb, emc, h, mab, Dl and E(spl)), previously characterized by mutations with large effects on bristle number.

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Selected References

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  1. Aguadé M., Meyers W., Long A. D., Langley C. H. Single-strand conformation polymorphism analysis coupled with stratified DNA sequencing reveals reduced sequence variation in the su(s) and su(wa) regions of the Drosophila melanogaster X chromosome. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4658–4662. doi: 10.1073/pnas.91.11.4658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barton N. H., Turelli M. Evolutionary quantitative genetics: how little do we know? Annu Rev Genet. 1989;23:337–370. doi: 10.1146/annurev.ge.23.120189.002005. [DOI] [PubMed] [Google Scholar]
  3. Campos-Ortega J. A., Jan Y. N. Genetic and molecular bases of neurogenesis in Drosophila melanogaster. Annu Rev Neurosci. 1991;14:399–420. doi: 10.1146/annurev.ne.14.030191.002151. [DOI] [PubMed] [Google Scholar]
  4. Davies R. W. The genetic relationship of two quantitative characters in Drosophila melanogaster. II. Location of the effects. Genetics. 1971 Nov;69(3):363–375. doi: 10.1093/genetics/69.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edwards M. D., Stuber C. W., Wendel J. F. Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics. 1987 May;116(1):113–125. doi: 10.1093/genetics/116.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frankham R., Jones L. P., Barker J. S. The effects of population size and selection intensity in selection for a quantitative character in Drosophila. I. Short-term response to selection. Genet Res. 1968 Dec;12(3):237–248. doi: 10.1017/s0016672300011848. [DOI] [PubMed] [Google Scholar]
  7. Fry J. D., deRonde K. A., Mackay T. F. Polygenic mutation in Drosophila melanogaster: genetic analysis of selection lines. Genetics. 1995 Mar;139(3):1293–1307. doi: 10.1093/genetics/139.3.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones K. R., Rubin G. M. Molecular analysis of no-on-transient A, a gene required for normal vision in Drosophila. Neuron. 1990 May;4(5):711–723. doi: 10.1016/0896-6273(90)90197-n. [DOI] [PubMed] [Google Scholar]
  9. Levin L. R., Han P. L., Hwang P. M., Feinstein P. G., Davis R. L., Reed R. R. The Drosophila learning and memory gene rutabaga encodes a Ca2+/Calmodulin-responsive adenylyl cyclase. Cell. 1992 Feb 7;68(3):479–489. doi: 10.1016/0092-8674(92)90185-f. [DOI] [PubMed] [Google Scholar]
  10. Mackay T. F., Fry J. D., Lyman R. F., Nuzhdin S. V. Polygenic mutation in Drosophila melanogaster: estimates from response to selection of inbred strains. Genetics. 1994 Mar;136(3):937–951. doi: 10.1093/genetics/136.3.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mackay T. F., Langley C. H. Molecular and phenotypic variation in the achaete-scute region of Drosophila melanogaster. Nature. 1990 Nov 1;348(6296):64–66. doi: 10.1038/348064a0. [DOI] [PubMed] [Google Scholar]
  12. Mackay T. F., Lyman R. F., Jackson M. S. Effects of P element insertions on quantitative traits in Drosophila melanogaster. Genetics. 1992 Feb;130(2):315–332. doi: 10.1093/genetics/130.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McMillan I., Robertson A. The power of methods for the detection of major genes affecting quantitative characters. Heredity (Edinb) 1974 Jun;32(3):349–356. doi: 10.1038/hdy.1974.43. [DOI] [PubMed] [Google Scholar]
  14. Meyerowitz E. M., Hogness D. S. Molecular organization of a Drosophila puff site that responds to ecdysone. Cell. 1982 Jan;28(1):165–176. doi: 10.1016/0092-8674(82)90386-5. [DOI] [PubMed] [Google Scholar]
  15. Montgomery E. A., Langley C. H. Transposable Elements in Mendelian Populations. II. Distribution of Three COPIA-like Elements in a Natural Population of DROSOPHILA MELANOGASTER. Genetics. 1983 Jul;104(3):473–483. doi: 10.1093/genetics/104.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Montgomery E., Charlesworth B., Langley C. H. A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster. Genet Res. 1987 Feb;49(1):31–41. doi: 10.1017/s0016672300026707. [DOI] [PubMed] [Google Scholar]
  17. Paterson A. H., Damon S., Hewitt J. D., Zamir D., Rabinowitch H. D., Lincoln S. E., Lander E. S., Tanksley S. D. Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics. 1991 Jan;127(1):181–197. doi: 10.1093/genetics/127.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Paterson A. H., DeVerna J. W., Lanini B., Tanksley S. D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics. 1990 Mar;124(3):735–742. doi: 10.1093/genetics/124.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sax K. The Association of Size Differences with Seed-Coat Pattern and Pigmentation in PHASEOLUS VULGARIS. Genetics. 1923 Nov;8(6):552–560. doi: 10.1093/genetics/8.6.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scherer G., Tschudi C., Perera J., Delius H., Pirrotta V. B104, a new dispersed repeated gene family in Drosophila melanogaster and its analogies with retroviruses. J Mol Biol. 1982 May 25;157(3):435–451. doi: 10.1016/0022-2836(82)90470-3. [DOI] [PubMed] [Google Scholar]
  21. Shrimpton A. E., Montgomery E. A., Langley C. H. OM Mutations in DROSOPHILA ANANASSAE Are Linked to Insertions of a Transposable Element. Genetics. 1986 Sep;114(1):125–135. doi: 10.1093/genetics/114.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith D. F., McClelland A., White B. N., Addison C. F., Glover D. M. The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of D. melanogaster. Cell. 1981 Feb;23(2):441–449. doi: 10.1016/0092-8674(81)90139-2. [DOI] [PubMed] [Google Scholar]
  23. Spickett S. G., Thoday J. M. Regular responses to selection. 3. Interaction between located polygenes. Genet Res. 1966 Feb;7(1):96–121. doi: 10.1017/s0016672300009502. [DOI] [PubMed] [Google Scholar]
  24. Turelli M. Heritable genetic variation via mutation-selection balance: Lerch's zeta meets the abdominal bristle. Theor Popul Biol. 1984 Apr;25(2):138–193. doi: 10.1016/0040-5809(84)90017-0. [DOI] [PubMed] [Google Scholar]
  25. WOLSTENHOLME D. R., THODAY J. M. EFFECTS OF DISRUPTIVE SELECTION. VII. A THIRD CHROMOSOME POLYMORPHISM. Heredity (Edinb) 1963 Nov;18:413–431. doi: 10.1038/hdy.1963.48. [DOI] [PubMed] [Google Scholar]
  26. Zeng Z. B., Houle D., Cockerham C. C. How informative is Wright's estimator of the number of genes affecting a quantitative character? Genetics. 1990 Sep;126(1):235–247. doi: 10.1093/genetics/126.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]

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