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. 1965 Feb;94(2):401–409. doi: 10.1042/bj0940401

The effect of chondromucoprotein on the solubility and peroxidatic properties of haemoglobin and methaemalbumin in aqueous solution and blood

A J Anderson 1
PMCID: PMC1206522  PMID: 14348200

Abstract

1. Insoluble complexes, formed by electrostatic interaction between chondromucoprotein and chromoproteins (haemoglobin, methaemalbumin), were studied by measurement of precipitated pigment and by decrease in peroxidatic activity, maximum formation from aqueous solution occurring at pH 4·0–4·6. 2. Chondromucoprotein did not form complexes with plasma haptoglobins and haptohaemoglobins under these conditions, and high concentrations had no significant effect on colorimetric estimates of serum haptoglobin, although the peroxidatic activity of haemoglobinaemic serum was depressed owing to formation of chondromucoprotein–methaemalbumin complex. 3. The complexes formed by interaction between chondromucoprotein and plasma proteins contain two protein-bound biologically active components (plasminogen, haematin), as a result of co-precipitation after interaction between their carriers and chondromucoprotein. The possible presence of other biologically active trace components is discussed. 4. The results are related to complex-formation between other plasma proteins and chondromucoprotein, and possible implications arising from the complex-forming properties of tissue and urine chondromucoprotein are referred to. It is concluded that the inability of chondromucoprotein to form complexes with normal urine proteins is due to a deficiency of fibrinogen, β-lipoproteins and chromoproteins, which, in plasma, form a large proportion of the proteins involved in complex-formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., REES W. A. The binding of haemoglobin by plasma proteins (haptoglobins); its bearing on the renal threshold for haemoglobin and the aetiology of haemoglobinuria. Br Med J. 1957 Nov 16;2(5054):1137–1143. doi: 10.1136/bmj.2.5054.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANDERSON A. J., LACK C. H. THE FORMATION, COMPOSITION AND FIBRINOLYTIC POTENTIAL OF CHONDROMUCOPROTEIN-FIBRINOGEN AND CHONDROMUCOPROTEIN-LIPOPROTEIN COMPLEXES IN HUMAN EUGLOBULIN FRACTIONS. Clin Sci. 1964 Feb;26:97–110. [PubMed] [Google Scholar]
  3. ANDERSON A. J., LEPPER M. H., WINZLER R. J. The fractionation of urine colloids on anion-exchange cellulose. Biochem J. 1960 Dec;77:581–591. doi: 10.1042/bj0770581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ANDERSON A. J., MACLAGAN N. F. The isolation and estimation of urinary mucoproteins. Biochem J. 1955 Apr;59(4):638–644. doi: 10.1042/bj0590638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ANDERSON A. J. Some studies on the occurrence of sialic acid in human cartilage. Biochem J. 1961 Feb;78:399–409. doi: 10.1042/bj0780399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ANDERSON A. J. Some studies on the relationship between sialic acid and the mucopolysaccharide-protein complexes in human cartilage. Biochem J. 1962 Feb;82:372–381. doi: 10.1042/bj0820372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ANDERSON A. J. THE FORMATION OF CHONDROMUCOPROTEIN-FIBRINOGEN AND CHONDROMUCOPROTEIN-BETA-LIPOPROTEIN COMPLEXES. Biochem J. 1963 Sep;88:460–469. doi: 10.1042/bj0880460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BOYCE W. H., KING J. S., Jr, LITTLE J. M., ARTOM C. Total nondialyzable solids (TNDS) in human urine. II. A method for reproducible fractionation. J Clin Invest. 1958 Nov;37(11):1658–1665. doi: 10.1172/JCI103757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CONNELL G. E., SMITHIES O. Human haptoglobins: estimation and purification. Biochem J. 1959 May;72(1):115–121. doi: 10.1042/bj0720115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. CROSBY W. H., FURTH F. W. A modification of the benzidine method for measurement of hemoglobin in plasma and urine. Blood. 1956 Apr;11(4):380–383. [PubMed] [Google Scholar]
  11. Huehns E. R., Shooter E. M., Beaven G. H. The time course of the recombination of human adult and canine haemoglobins. Biochem J. 1964 May;91(2):331–334. doi: 10.1042/bj0910331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JAYLE M. F., BOUSSIER G., BADIN J. Electrophorèse de l'haptoglobine et de son complexe hémoglobinique. Bull Soc Chim Biol (Paris) 1952;34(11):1063–1069. [PubMed] [Google Scholar]
  13. LAWRENCE S. H., MELNICK P. J. Enzymatic activity related to human serum beta-lipoprotein: histochemical, immunoelectrophoretic and quantitative studies. Proc Soc Exp Biol Med. 1961 Aug-Sep;107:998–1001. [PubMed] [Google Scholar]
  14. LIANG C. C. The formation of complexes between haemoglobins and plasma proteins in a variety of animals. Biochem J. 1957 Aug;66(4):552–558. doi: 10.1042/bj0660552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LUPOVITCH A., ZAK B. A STUDY ON SERUM HEMOGLOBIN AND HEMOGLOBIN-BINDING CAPACITY. Clin Chim Acta. 1964 Jan;9:49–55. doi: 10.1016/0009-8981(64)90043-9. [DOI] [PubMed] [Google Scholar]
  16. OERTEL G. W., KAISER E. [On steroid conjugates in plasma. III. Quantitative determination of non-polar 17-ketosteroid conjugates in human plasma]. Clin Chim Acta. 1962 Jul;7:463–466. doi: 10.1016/0009-8981(62)90085-2. [DOI] [PubMed] [Google Scholar]
  17. ROBERT L., CROSTI P. EFFECTS OF CRUDE PANCREATIC ELASTASE ON SERUM HAPTOGLOBIN. Clin Chim Acta. 1964 Jan;9:56–63. doi: 10.1016/0009-8981(64)90044-0. [DOI] [PubMed] [Google Scholar]
  18. ROBERT L., MOMBELLONI P., CROSTI P. Studies on serum haptoglobin in experimental connective tissue disorders. Proc Soc Exp Biol Med. 1961 Jul;107:499–502. doi: 10.3181/00379727-107-26667. [DOI] [PubMed] [Google Scholar]
  19. SHINOWARA G. Y., WALTERS M. I. HEMATIN--STUDIES ON PROTEIN COMPLEXES AND DETERMINATION IN HUMAN PLASMA. Am J Clin Pathol. 1963 Aug;40:113–122. doi: 10.1093/ajcp/40.2.113. [DOI] [PubMed] [Google Scholar]
  20. SMITHIES O. Grouped variations in the occurrence of new protein components in normal human serum. Nature. 1955 Feb 12;175(4450):307–308. doi: 10.1038/175307a0. [DOI] [PubMed] [Google Scholar]
  21. Smith H., Owen J. A. The determination of haptoglobins in normal human serum. Biochem J. 1961 Apr;78(4):723–728. doi: 10.1042/bj0780723. [DOI] [PMC free article] [PubMed] [Google Scholar]

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