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. 1965 Mar;94(3):692–699. doi: 10.1042/bj0940692

The adenosine-triphosphatase system responsible for cation transport in electric organ: exclusion of phospholipids as intermediates

I M Glynn 1,2, Carolyn W Slayman 1,2,*, J Eichberg 1,2,†, R M C Dawson 1,2
PMCID: PMC1206604  PMID: 14340060

Abstract

1. Subcellular fractions were prepared from the electric organs of Electrophorus and Torpedo and assayed for adenosine-triphosphatase activity. 2. Treatment of the `low-speed' fraction from Torpedo with m-urea gave an adenosine-triphosphatase preparation that was almost completely (98%) inhibited by ouabain (0·1mg./ml.) and dependent on the simultaneous presence of Na+ and K+. 3. The adenosine-triphosphatase preparations were exposed to [γ-32P]ATP for 30sec. in the presence of (i) Na+, (ii) K+, (iii) Na++K+ and (iv) Na++K++ouabain. No significant labelling of phosphatidic acid, triphosphoinositide or any other phospholipid was observed. 4. The results suggest that phospholipids do not act as phosphorylated intermediates in the `transport adenosine-triphosphatase' system of electric organ.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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