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. 1965 Mar;94(3):755–759. doi: 10.1042/bj0940755

Biosynthesis of riboflavine by a purine-requiring mutant strain of Escherichia coli

D J Howells 1, G W E Plaut 1
PMCID: PMC1206612  PMID: 14340067

Abstract

1. Riboflavine biosynthesis occurs in non-proliferating cultures of a purine-requiring strain of Escherichia coli (ATCC no. 13863). 2. No significant incorporation of radioactivity from [1-14C]glycine into either C-4a and C-9a of riboflavine or into nucleic acid purines is detected under the above conditions; appreciable incorporation of label into 5-aminoimidazole-4-carboxamide occurs. However, the label of [6-14C]guanine is incorporated significantly into C-4 of riboflavine and into nucleic acid adenine and guanine; the specific radioactivity of the riboflavine is approximately twice that of either adenine or guanine of nucleic acid. 3. These results show that a purine derivative is an obligatory intermediate in riboflavine biogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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