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. 1995 Jun;140(2):703–724. doi: 10.1093/genetics/140.2.703

An Autosomal Genetic Linkage Map of the Sheep Genome

A M Crawford 1, K G Dodds 1, A J Ede 1, C A Pierson 1, G W Montgomery 1, H G Garmonsway 1, A E Beattie 1, K Davies 1, J F Maddox 1, S W Kappes 1, R T Stone 1, T C Nguyen 1, J M Penty 1, E A Lord 1, J E Broom 1, J Buitkamp 1, W Schwaiger 1, J T Epplen 1, P Matthew 1, M E Matthews 1, D J Hulme 1, K J Beh 1, R A McGraw 1, C W Beattie 1
PMCID: PMC1206646  PMID: 7498748

Abstract

We report the first extensive ovine genetic linkage map covering 2070 cM of the sheep genome. The map was generated from the linkage analysis of 246 polymorphic markers, in nine three-generation fullsib pedigrees, which make up the AgResearch International Mapping Flock. We have exploited many markers from cattle so that valuable comparisons between these two ruminant linkage maps can be made. The markers, used in the segregation analyses, comprised 86 anonymous microsatellite markers derived from the sheep genome, 126 anonymous microsatellites from cattle, one from deer, and 33 polymorphic markers of various types associated with known genes. The maximum number of informative meioses within the mapping flock was 222. The average number of informative meioses per marker was 140 (range 18-209). Linkage groups have been assigned to all 26 sheep autosomes.

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Selected References

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  1. Ansari H. A., Pearce P. D., Maher D. W., Broad T. E. Regional assignment of conserved reference loci anchors unassigned linkage and syntenic groups to ovine chromosomes. Genomics. 1994 Dec;24(3):451–455. doi: 10.1006/geno.1994.1652. [DOI] [PubMed] [Google Scholar]
  2. Avraham A., Band M., Yoffe O., Shani M., Ron M. Bovine dinucleotide repeat polymorphism at the ARO28 locus. Anim Genet. 1993 Apr;24(2):147–147. [PubMed] [Google Scholar]
  3. Barendse W., Armitage S. M., Kossarek L. M., Shalom A., Kirkpatrick B. W., Ryan A. M., Clayton D., Li L., Neibergs H. L., Zhang N. A genetic linkage map of the bovine genome. Nat Genet. 1994 Mar;6(3):227–235. doi: 10.1038/ng0394-227. [DOI] [PubMed] [Google Scholar]
  4. Blattman A. N., Beh K. J. Dinucleotide repeat polymorphism within the ovine major histocompatibility complex. Anim Genet. 1992;23(4):392–392. doi: 10.1111/j.1365-2052.1992.tb00165.x. [DOI] [PubMed] [Google Scholar]
  5. Brezinsky L., Kemp S. J., Teale A. J. Five polymorphic bovine microsatellites (ILSTS010-014). Anim Genet. 1993 Feb;24(1):75–76. doi: 10.1111/j.1365-2052.1993.tb00936.x. [DOI] [PubMed] [Google Scholar]
  6. Buchanan F. C., Adams L. J., Littlejohn R. P., Maddox J. F., Crawford A. M. Determination of evolutionary relationships among sheep breeds using microsatellites. Genomics. 1994 Jul 15;22(2):397–403. doi: 10.1006/geno.1994.1401. [DOI] [PubMed] [Google Scholar]
  7. Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF209 locus. Anim Genet. 1992;23(2):183–183. doi: 10.1111/j.1365-2052.1992.tb00041.x. [DOI] [PubMed] [Google Scholar]
  8. Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF33 locus. Anim Genet. 1992;23(2):186–186. doi: 10.1111/j.1365-2052.1992.tb00044.x. [DOI] [PubMed] [Google Scholar]
  9. Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF70 locus. Anim Genet. 1992;23(2):185–185. doi: 10.1111/j.1365-2052.1992.tb00043.x. [DOI] [PubMed] [Google Scholar]
  10. Buchanan F. C., Crawford A. M. Ovine microsatellites at the OarFCB11, OarFCB128, OarFCB193, OarFCB266 and OarFCB304 loci. Anim Genet. 1993 Apr;24(2):145–145. doi: 10.1111/j.1365-2052.1993.tb00269.x. [DOI] [PubMed] [Google Scholar]
  11. Buchanan F. C., Galloway S. M., Crawford A. M. Ovine microsatellites at the OarFCB5, OarFCB19, OarFCB20, OarFCB48, OarFCB129 and OarFCB226 loci. Anim Genet. 1994 Feb;25(1):60–60. [PubMed] [Google Scholar]
  12. Buchanan F. C., Littlejohn R. P., Galloway S. M., Crawford A. M. Microsatellites and associated repetitive elements in the sheep genome. Mamm Genome. 1993;4(5):258–264. doi: 10.1007/BF00417432. [DOI] [PubMed] [Google Scholar]
  13. Buchanan F. C., Swarbrick P. A., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF4 locus. Anim Genet. 1991;22(4):373–374. doi: 10.1111/j.1365-2052.1991.tb00694.x. [DOI] [PubMed] [Google Scholar]
  14. Buchanan F. C., Swarbrick P. A., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF48 locus. Anim Genet. 1991;22(4):379–380. doi: 10.1111/j.1365-2052.1991.tb00697.x. [DOI] [PubMed] [Google Scholar]
  15. Buitkamp J., Schwaiger F. W., Epplen J. T. Vb6 T-cell receptor elements in artiodactyls: conservation and germline polymorphisms. Mamm Genome. 1993 Sep;4(9):504–510. doi: 10.1007/BF00364785. [DOI] [PubMed] [Google Scholar]
  16. Burkin D. J., Morse H. G., Broad T. E., Pearce P. D., Ansari H. A., Lewis P. E., Jones C. Mapping the sheep genome: production of characterized sheep x hamster cell hybrids. Genomics. 1993 May;16(2):466–472. doi: 10.1006/geno.1993.1212. [DOI] [PubMed] [Google Scholar]
  17. Chapman H. M., Bruère A. N. Chromosome morphology during meiosis of normal and Robertsonian translocation-carrying rams (Ovis aries). Can J Genet Cytol. 1977 Mar;19(1):93–102. doi: 10.1139/g77-011. [DOI] [PubMed] [Google Scholar]
  18. Cockett N. E., Jackson S. P., Shay T. L., Nielsen D., Moore S. S., Steele M. R., Barendse W., Green R. D., Georges M. Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3019–3023. doi: 10.1073/pnas.91.8.3019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Copeland N. G., Jenkins N. A., Gilbert D. J., Eppig J. T., Maltais L. J., Miller J. C., Dietrich W. F., Weaver A., Lincoln S. E., Steen R. G. A genetic linkage map of the mouse: current applications and future prospects. Science. 1993 Oct 1;262(5130):57–66. doi: 10.1126/science.8211130. [DOI] [PubMed] [Google Scholar]
  20. Crawford A. M., Buchanan F. C., Swarbrick P. A. Ovine dinucleotide repeat polymorphism at the MAF92 locus. Anim Genet. 1991;22(4):371–372. doi: 10.1111/j.1365-2052.1991.tb00693.x. [DOI] [PubMed] [Google Scholar]
  21. Crawford A. M., Montgomery G. W., Pierson C. A., Brown T., Dodds K. G., Sunden S. L., Henry H. M., Ede A. J., Swarbrick P. A., Berryman T. Sheep linkage mapping: nineteen linkage groups derived from the analysis of paternal half-sib families. Genetics. 1994 Jun;137(2):573–579. doi: 10.1093/genetics/137.2.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Davies K. P., Maddox J. F. Ovine dinucleotide repeat polymorphism at the lysozyme (LYZ) locus. Anim Genet. 1994 Jun;25(3):204–204. doi: 10.1111/j.1365-2052.1994.tb00127.x. [DOI] [PubMed] [Google Scholar]
  23. Dietrich W. F., Miller J. C., Steen R. G., Merchant M., Damron D., Nahf R., Gross A., Joyce D. C., Wessel M., Dredge R. D. A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nat Genet. 1994 Jun;7(2 Spec No):220–245. doi: 10.1038/ng0694supp-220. [DOI] [PubMed] [Google Scholar]
  24. Echard G., Broad T. E., Hill D., Pearce P. Present status of the ovine gene map (Ovis aries); comparison with the bovine map (Bos taurus). Mamm Genome. 1994 Jun;5(6):324–332. doi: 10.1007/BF00356549. [DOI] [PubMed] [Google Scholar]
  25. Ede A. J., Crawford A. M. Mutations in the sequence flanking the microsatellite at the KAP8 locus prevent the amplification of some alleles. Anim Genet. 1995 Feb;26(1):43–44. doi: 10.1111/j.1365-2052.1995.tb02619.x. [DOI] [PubMed] [Google Scholar]
  26. Ede A. J., Peirson C. A., Henry H., Crawford A. M. Ovine microsatellites at the OarAE64, OarHH22, OarHH56, OarHH62 and OarVH4 loci. Anim Genet. 1994 Feb;25(1):51–52. [PubMed] [Google Scholar]
  27. Fries R., Eggen A., Womack J. E. The bovine genome map. Mamm Genome. 1993;4(8):405–428. doi: 10.1007/BF00296815. [DOI] [PubMed] [Google Scholar]
  28. Gyapay G., Morissette J., Vignal A., Dib C., Fizames C., Millasseau P., Marc S., Bernardi G., Lathrop M., Weissenbach J. The 1993-94 Généthon human genetic linkage map. Nat Genet. 1994 Jun;7(2 Spec No):246–339. doi: 10.1038/ng0694supp-246. [DOI] [PubMed] [Google Scholar]
  29. Hanrahan V., Ede A. J., Pierson C. A., Hill D. F. Ovine microsatellites at the OarVH98, OarVH110, OarVH116, OarVH117 and OarVH130 loci. Anim Genet. 1993 Jun;24(3):223–223. doi: 10.1111/j.1365-2052.1993.tb00301.x. [DOI] [PubMed] [Google Scholar]
  30. Hanrahan V., Ede A. J., Pierson C. A., Hill D. F. Ovine microsatellites at the OarVH98, OarVH110, OarVH116, OarVH117 and OarVH130 loci. Anim Genet. 1993 Jun;24(3):223–223. doi: 10.1111/j.1365-2052.1993.tb00301.x. [DOI] [PubMed] [Google Scholar]
  31. Hawken R. J., Davies K. P., Maddox J. F. A dinucleotide repeat in the ovine interleukin-3 gene of limited polymorphism. Anim Genet. 1994 Aug;25(4):286–286. doi: 10.1111/j.1365-2052.1994.tb00210.x. [DOI] [PubMed] [Google Scholar]
  32. Hawken R. J., Davies K. P., Maddox J. F. A polymorphic tetranucleotide repeat in the ovine metallothionein II gene. Anim Genet. 1994 Oct;25(5):366–366. doi: 10.1111/j.1365-2052.1994.tb00377.x. [DOI] [PubMed] [Google Scholar]
  33. Hediger R., Ansari H. A., Stranzinger G. F. Chromosome banding and gene localizations support extensive conservation of chromosome structure between cattle and sheep. Cytogenet Cell Genet. 1991;57(2-3):127–134. doi: 10.1159/000133131. [DOI] [PubMed] [Google Scholar]
  34. Kaukinen J., Varvio S. L. Eight polymorphic bovine microsatellites. Anim Genet. 1993 Apr;24(2):148–148. doi: 10.1111/j.1365-2052.1993.tb00274.x. [DOI] [PubMed] [Google Scholar]
  35. Keats B. J., Sherman S. L., Morton N. E., Robson E. B., Buetow K. H., Cartwright P. E., Chakravarti A., Francke U., Green P. P., Ott J. Guidelines for human linkage maps: an international system for human linkage maps (ISLM, 1990). Genomics. 1991 Mar;9(3):557–560. doi: 10.1016/0888-7543(91)90426-f. [DOI] [PubMed] [Google Scholar]
  36. Kemp S. J., Brezinsky L., Teale A. J. ILSTS002: a polymorphic bovine microsatellite. Anim Genet. 1992;23(2):184–184. doi: 10.1111/j.1365-2052.1992.tb00042.x. [DOI] [PubMed] [Google Scholar]
  37. Kossarek L. M., Grosse W. M., Dietz A. B., Womack J. E., McGraw R. A. Rapid communication: bovine dinucleotide repeat polymorphism RM004. J Anim Sci. 1993 Nov;71(11):3175–3175. doi: 10.2527/1993.71113175x. [DOI] [PubMed] [Google Scholar]
  38. Kossarek L. M., Grosse W. M., Finlay O., McGraw R. A. Rapid communication: bovine dinucleotide repeat polymorphism RM006. J Anim Sci. 1993 Nov;71(11):3176–3176. doi: 10.2527/1993.71113176x. [DOI] [PubMed] [Google Scholar]
  39. Litt M., Luty J. A. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet. 1989 Mar;44(3):397–401. [PMC free article] [PubMed] [Google Scholar]
  40. Matthew P., Maddox J. F. A polymorphic dinucleotide repeat microsatellite detected in the ovine interleukin 2 receptor alpha (IL2RA, CD25) gene. Anim Genet. 1994 Jun;25(3):200–200. doi: 10.1111/j.1365-2052.1994.tb00121.x. [DOI] [PubMed] [Google Scholar]
  41. Matthew P., Maddox J. F. A polymorphic microsatellite detected in the ovine CD5 gene. Anim Genet. 1994 Oct;25(5):371–371. doi: 10.1111/j.1365-2052.1994.tb00385.x. [DOI] [PubMed] [Google Scholar]
  42. Montgomery G. W., Crawford A. M., Penty J. M., Dodds K. G., Ede A. J., Henry H. M., Pierson C. A., Lord E. A., Galloway S. M., Schmack A. E. The ovine Booroola fecundity gene (FecB) is linked to markers from a region of human chromosome 4q. Nat Genet. 1993 Aug;4(4):410–414. doi: 10.1038/ng0893-410. [DOI] [PubMed] [Google Scholar]
  43. Montgomery G. W., Lord E. A., Penty J. M., Dodds K. G., Broad T. E., Cambridge L., Sunden S. L., Stone R. T., Crawford A. M. The Booroola fecundity (FecB) gene maps to sheep chromosome 6. Genomics. 1994 Jul 1;22(1):148–153. doi: 10.1006/geno.1994.1355. [DOI] [PubMed] [Google Scholar]
  44. Montgomery G. W., Sise J. A., Penty J. M., Tou H. M., Hill D. F. Sheep linkage mapping: restriction fragment length polymorphism detection with heterologous cDNA probes. Anim Genet. 1992;23(5):411–416. doi: 10.1111/j.1365-2052.1992.tb02159.x. [DOI] [PubMed] [Google Scholar]
  45. Moore S. S., Barendse W., Berger K. T., Armitage S. M., Hetzel D. J. Bovine and ovine DNA microsatellites from the EMBL and GENBANK databases. Anim Genet. 1992;23(5):463–467. doi: 10.1111/j.1365-2052.1992.tb02168.x. [DOI] [PubMed] [Google Scholar]
  46. Moore S. S., Byrne K., Berger K. T., Barendse W., McCarthy F., Womack J. E., Hetzel D. J. Characterization of 65 bovine microsatellites. Mamm Genome. 1994 Feb;5(2):84–90. doi: 10.1007/BF00292333. [DOI] [PubMed] [Google Scholar]
  47. Moore S. S., Byrne K. Dinucleotide polymorphism at the bovine calmodulin independent adenylcyclase locus. Anim Genet. 1993 Apr;24(2):150–150. doi: 10.1111/j.1365-2052.1993.tb00278.x. [DOI] [PubMed] [Google Scholar]
  48. O'Brien S. J., Womack J. E., Lyons L. A., Moore K. J., Jenkins N. A., Copeland N. G. Anchored reference loci for comparative genome mapping in mammals. Nat Genet. 1993 Feb;3(2):103–112. doi: 10.1038/ng0293-103. [DOI] [PubMed] [Google Scholar]
  49. Penty J. M., Henry H. M., Ede A. J., Crawford A. M. Ovine microsatellites at the OarAE16, OarAE54, OarAE57, OarAE119 and OarAE129 loci. Anim Genet. 1993 Jun;24(3):219–219. doi: 10.1111/j.1365-2052.1993.tb00295.x. [DOI] [PubMed] [Google Scholar]
  50. Penty J. M., Montgomery G. W. An Xba RFLP at the ovine basic fibroblast growth factor locus (FGFB). Anim Genet. 1993 Aug;24(4):329–329. doi: 10.1111/j.1365-2052.1993.tb00328.x. [DOI] [PubMed] [Google Scholar]
  51. Pierson C. A., Hanrahan V., Ede A. J., Crawford A. M. Ovine microsatellites at the OarVH34, OarVH41, OarVH58, OarVH61 and OarVH72 loci. Anim Genet. 1993 Jun;24(3):224–224. doi: 10.1111/j.1365-2052.1993.tb00302.x. [DOI] [PubMed] [Google Scholar]
  52. Primmer C. R., Matthews M. E. Ovine dinucleotide repeat polymorphism at the VIAS-S5 locus. Anim Genet. 1993 Apr;24(2):146–146. doi: 10.1111/j.1365-2052.1993.tb00270.x. [DOI] [PubMed] [Google Scholar]
  53. Rohrer G. A., Alexander L. J., Keele J. W., Smith T. P., Beattie C. W. A microsatellite linkage map of the porcine genome. Genetics. 1994 Jan;136(1):231–245. doi: 10.1093/genetics/136.1.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  55. Schwaiger F. W., Buitkamp J., Weyers E., Epplen J. T. Typing of artiodactyl MHC-DRB genes with the help of intronic simple repeated DNA sequences. Mol Ecol. 1993 Feb;2(1):55–59. doi: 10.1111/j.1365-294x.1993.tb00099.x. [DOI] [PubMed] [Google Scholar]
  56. Shalom A., Mosig M. O., Barendse W., Friedmann A., Soller M. Dinucleotide repeat polymorphism at the bovine HUJ246, HUJII77, HUJ223, HUJVI74 and HUJI75 loci. Anim Genet. 1994 Feb;25(1):56–56. [PubMed] [Google Scholar]
  57. Steffen P., Eggen A., Dietz A. B., Womack J. E., Stranzinger G., Fries R. Isolation and mapping of polymorphic microsatellites in cattle. Anim Genet. 1993 Apr;24(2):121–124. doi: 10.1111/j.1365-2052.1993.tb00252.x. [DOI] [PubMed] [Google Scholar]
  58. Sun H. S., Dentine M. R., Kirkpatrick B. W. A polymorphic microsatellite (UWCA4) detected on bovine chromosome 21. Anim Genet. 1993 Apr;24(2):149–149. doi: 10.1111/j.1365-2052.1993.tb00275.x. [DOI] [PubMed] [Google Scholar]
  59. Sun H. S., Huff B. M., Dentine M. R., Kirkpatrick B. W. Polymorphic bovine microsatellites UWCA5, UWCA7 and UWCA9. Anim Genet. 1994 Feb;25(1):59–59. [PubMed] [Google Scholar]
  60. Swarbrick P. A., Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF23 locus. Anim Genet. 1991;22(2):191–191. doi: 10.1111/j.1365-2052.1991.tb00663.x. [DOI] [PubMed] [Google Scholar]
  61. Swarbrick P. A., Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF35 locus. Anim Genet. 1991;22(4):369–370. doi: 10.1111/j.1365-2052.1991.tb00692.x. [DOI] [PubMed] [Google Scholar]
  62. Swarbrick P. A., Buchanan F. C., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF36 locus. Anim Genet. 1991;22(4):377–378. doi: 10.1111/j.1365-2052.1991.tb00696.x. [DOI] [PubMed] [Google Scholar]
  63. Swarbrick P. A., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF109 locus. Anim Genet. 1992;23(1):84–84. [PubMed] [Google Scholar]
  64. Swarbrick P. A., Howes J., Crawford A. M. Ovine dinucleotide repeat polymorphism at the MAF50 locus. Anim Genet. 1992;23(2):187–187. doi: 10.1111/j.1365-2052.1992.tb00045.x. [DOI] [PubMed] [Google Scholar]
  65. Vaiman D., Osta R., Mercier D., Grohs C., Levéziel H. Characterization of five new bovine dinucleotide repeats. Anim Genet. 1992;23(6):537–541. doi: 10.1111/j.1365-2052.1992.tb00175.x. [DOI] [PubMed] [Google Scholar]
  66. Weber J. L., May P. E. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet. 1989 Mar;44(3):388–396. [PMC free article] [PubMed] [Google Scholar]
  67. Wood N. J., Phua S. H. A dinucleotide repeat polymorphism at the adenylate cyclase activating polypeptide locus in sheep. Anim Genet. 1993 Aug;24(4):329–329. doi: 10.1111/j.1365-2052.1993.tb00329.x. [DOI] [PubMed] [Google Scholar]
  68. Wood N. J., Phua S. H., Crawford A. M. A dinucleotide repeat polymorphism at the glycine- and tyrosine-rich keratin locus in sheep. Anim Genet. 1992;23(4):391–391. doi: 10.1111/j.1365-2052.1992.tb00164.x. [DOI] [PubMed] [Google Scholar]
  69. Yamada J., Kuramoto T., Serikawa T. A rat genetic linkage map and comparative maps for mouse or human homologous rat genes. Mamm Genome. 1994 Feb;5(2):63–83. doi: 10.1007/BF00292332. [DOI] [PMC free article] [PubMed] [Google Scholar]

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