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. 1995 Jul;140(3):1007–1017. doi: 10.1093/genetics/140.3.1007

Distance and Pairing Effects on the Brown(dominant) Heterochromatic Element in Drosophila

S Henikoff 1, J M Jackson 1, P B Talbert 1
PMCID: PMC1206657  PMID: 7672573

Abstract

We examined the behavior of the brown(Dominant) (bw(D)) heterochromatic insertion moved to different locations relative to centric heterochromatin. Effects were measured as the degree of silencing of a wild-type brown eye pigment gene by bw(D) across a tandem duplication. A series of X-ray-induced effects were recovered at high frequency. Cis-acting enhancers were obtained by relocation of the duplication closer to autosomal heterochromatin. Enhancers were also recovered on the homologous chromosome when it was similarly rearranged, revealing a novel interhomologue effect whereby interactions occur between genetic elements near opposite ends of a chromosome arm rather than between paired alleles. Cis-acting suppressors were obtained as secondary rearrangements in which the duplication was moved farther away from heterochromatin. Suppression was correlated with loss of cytological association between bw(D) and the polytene chromocenter. Surprisingly, the distance from bw(D) to the chromocenter was not correlated with the strength of enhancement or suppression. We propose that bw(D) fails to coalesce with the chromocenter when its position along the chromosome places it beyond a threshold distance from heterochromatin, and this threshold depends upon the configuration of both the chromosome carrying bw(D) and its paired homologue.

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Selected References

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  1. Baker W K. V-Type Position Effects of a Gene in Drosophila Virilis Normally Located in Heterochromatin. Genetics. 1953 Jul;38(4):328–344. doi: 10.1093/genetics/38.4.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dorer D. R., Henikoff S. Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. Cell. 1994 Jul 1;77(7):993–1002. doi: 10.1016/0092-8674(94)90439-1. [DOI] [PubMed] [Google Scholar]
  3. Eberl D. F., Duyf B. J., Hilliker A. J. The role of heterochromatin in the expression of a heterochromatic gene, the rolled locus of Drosophila melanogaster. Genetics. 1993 May;134(1):277–292. doi: 10.1093/genetics/134.1.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eissenberg J. C. Position effect variegation in Drosophila: towards a genetics of chromatin assembly. Bioessays. 1989 Jul;11(1):14–17. doi: 10.1002/bies.950110105. [DOI] [PubMed] [Google Scholar]
  5. Ephrussi B., Sutton E. A Reconsideration of the Mechanism of Position Effect. Proc Natl Acad Sci U S A. 1944 Aug 15;30(8):183–197. doi: 10.1073/pnas.30.8.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gatti M., Pimpinelli S. Functional elements in Drosophila melanogaster heterochromatin. Annu Rev Genet. 1992;26:239–275. doi: 10.1146/annurev.ge.26.120192.001323. [DOI] [PubMed] [Google Scholar]
  7. Gelbart W. M. Synapsis-dependent allelic complementation at the decapentaplegic gene complex in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2636–2640. doi: 10.1073/pnas.79.8.2636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grigliatti T. Position-effect variegation--an assay for nonhistone chromosomal proteins and chromatin assembly and modifying factors. Methods Cell Biol. 1991;35:587–627. [PubMed] [Google Scholar]
  9. Gubb D., Ashburner M., Roote J., Davis T. A novel transvection phenomenon affecting the white gene of Drosophila melanogaster. Genetics. 1990 Sep;126(1):167–176. doi: 10.1093/genetics/126.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Henikoff S. Position-effect variegation after 60 years. Trends Genet. 1990 Dec;6(12):422–426. doi: 10.1016/0168-9525(90)90304-o. [DOI] [PubMed] [Google Scholar]
  11. Hilliker A. J., Holm D. G. Genetic analysis of the proximal region of chromosome 2 of Drosophila melanogaster. I. Detachment products of compound autosomes. Genetics. 1975 Dec;81(4):705–721. doi: 10.1093/genetics/81.4.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaufmann B P. Reversion from Roughest to Wild Type in Drosophila Melanogaster. Genetics. 1942 Sep;27(5):537–549. doi: 10.1093/genetics/27.5.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leiserson W. M., Bonini N. M., Benzer S. Transvection at the eyes absent gene of Drosophila. Genetics. 1994 Dec;138(4):1171–1179. doi: 10.1093/genetics/138.4.1171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lohe A. R., Hilliker A. J., Roberts P. A. Mapping simple repeated DNA sequences in heterochromatin of Drosophila melanogaster. Genetics. 1993 Aug;134(4):1149–1174. doi: 10.1093/genetics/134.4.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reuter G., Spierer P. Position effect variegation and chromatin proteins. Bioessays. 1992 Sep;14(9):605–612. doi: 10.1002/bies.950140907. [DOI] [PubMed] [Google Scholar]
  16. Slatis H M. Position Effects at the Brown Locus in Drosophila Melanogaster. Genetics. 1955 Jan;40(1):5–23. doi: 10.1093/genetics/40.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Talbert P. B., LeCiel C. D., Henikoff S. Modification of the Drosophila heterochromatic mutation brownDominant by linkage alterations. Genetics. 1994 Feb;136(2):559–571. doi: 10.1093/genetics/136.2.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tartof K. D., Henikoff S. Trans-sensing effects from Drosophila to humans. Cell. 1991 Apr 19;65(2):201–203. doi: 10.1016/0092-8674(91)90153-p. [DOI] [PubMed] [Google Scholar]
  19. Wakimoto B. T., Hearn M. G. The effects of chromosome rearrangements on the expression of heterochromatic genes in chromosome 2L of Drosophila melanogaster. Genetics. 1990 May;125(1):141–154. doi: 10.1093/genetics/125.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]

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