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. 1995 Jul;140(3):965–972. doi: 10.1093/genetics/140.3.965

DNA Synthesis Errors Associated with Double-Strand-Break Repair

J N Strathern 1, B K Shafer 1, C B McGill 1
PMCID: PMC1206680  PMID: 7672595

Abstract

Repair of a site-specific double-strand DNA break (DSB) resulted in increased reversion frequency for a nearby allele. Site-specific DSBs were introduced into the genome of Saccharomyces cerevisiae by the endonuclease encoded by the HO gene. Expression of the HO gene from a galactose-inducible promoter allowed efficient DNA cleavage at a single site in large populations of cells. To determine whether the DNA synthesis associated with repair of DSBs has a higher error rate than that associated with genome duplication, HO-induced DSBs were generated 0.3 kb from revertible alleles of trp1. The reversion rate of the trp1 alleles was ~100-fold higher among cells that had experienced an HO cut than among uninduced cells. The reverted allele was found predominantly on the chromosome that experienced the DNA cleavage.

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Selected References

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