Abstract
Inbreeding depression was observed in the commercial button mushroom, Agaricus bisporus, by examining two laboratory populations. The outbred population consisted of 20 compatible pairings, 10 homokaryons with each of the homokaryons Ag1-1 and Ag89-65. The inbred population consisted of 104 backcrosses (among which 52 were expected to be sexually compatible) obtained from the pairings of two progenitor homokaryons, Ag1-1 and Ag89-65, with 52 progeny homokaryons derived from the mating between Ag1-1 and Ag89-65. The eight fitness components examined for these two populations were successful matings as identified by the analysis of restriction fragment length polymorphisms, positive mycelial interaction in these successful matings, heterokaryon growth rate, primordium formation by the successful matings, fertile fruiting body formation, time to first break, average number of fruiting bodies per square foot, and average weight per fruiting body. The outcrossed population showed a significant advantage over the inbred population in three of eight fitness components. Two pairs of traits were significantly correlated. The multiplicative fitness ratio of the inbred to the outcrossed population was 0.18. The relevance of inbreeding depression to the evolution of fungal mating systems and to mushroom breeding is discussed.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Castle A. J., Horgen P. A., Anderson J. B. Crosses among Homokaryons from Commercial and Wild-Collected Strains of the Mushroom Agaricus brunnescens (= A. bisporus). Appl Environ Microbiol. 1988 Jul;54(7):1643–1648. doi: 10.1128/aem.54.7.1643-1648.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOBZHANSKY T., SPASSKY B., TIDWELL T. Genetics of natural populations. 32. Inbreeding and the mutational and balanced genetic loads in natural populations of Drosophila pseudoobscura. Genetics. 1963 Mar;48:361–373. doi: 10.1093/genetics/48.3.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jin T., Horgen P. A. Uniparental Mitochondrial Transmission in the Cultivated Button Mushroom, Agaricus bisporus. Appl Environ Microbiol. 1994 Dec;60(12):4456–4460. doi: 10.1128/aem.60.12.4456-4460.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jin T., Sonnenberg A. S., Van Griensven L. J., Horgen P. A. Investigation of Mitochondrial Transmission in Selected Matings between Homokaryons from Commercial and Wild-Collected Isolates of Agaricus bisporus (= Agaricus brunnescens). Appl Environ Microbiol. 1992 Nov;58(11):3553–3560. doi: 10.1128/aem.58.11.3553-3560.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kerrigan R. W., Royer J. C., Baller L. M., Kohli Y., Horgen P. A., Anderson J. B. Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus. Genetics. 1993 Feb;133(2):225–236. doi: 10.1093/genetics/133.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leslie J. F., Raju N. B. Recessive mutations from natural populations of Neurospora crassa that are expressed in the sexual diplophase. Genetics. 1985 Dec;111(4):759–777. doi: 10.1093/genetics/111.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- San Antonio J. P. A laboratory method to obtain fruit from cased grain spawn of the cultivated mushoom, Agaricus bisporus. Mycologia. 1971 Jan-Feb;63(1):16–21. [PubMed] [Google Scholar]
- Summerbell R. C., Castle A. J., Horgen P. A., Anderson J. B. Inheritance of restriction fragment length polymorphisms in Agaricus brunnescens. Genetics. 1989 Oct;123(2):293–300. doi: 10.1093/genetics/123.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Xu J., Kerrigan R. W., Horgen P. A., Anderson J. B. Localization of the Mating Type Gene in Agaricus bisporus. Appl Environ Microbiol. 1993 Sep;59(9):3044–3049. doi: 10.1128/aem.59.9.3044-3049.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]