Abstract
Chaser (Csr) was uncovered in a gamma mutagenesis screen to identify genes that modify the larval foraging behavior of sitters to rovers. Rover larvae have significantly longer path lenghts than sitters while foraging on a yeast and water paste. This difference is influenced by one major gene, foraging (for), which has two naturally occurring alleles, for(R) (rover) and for(s) (sitter). In a mutagenesis screen for modifiers of for, we identified three lines with viable mutations on chromosome 3 that alter foraging behavior. Each of these mutations increased larval path lengths in for(s)/for(s) larvae in a dominant fashion, and were not separable by recombination. These mutations are therefore probably allelic and define a new gene that we have called Csr. Csr was genetically localized using the lethal-tagging technique. This technique resulted in seven lines with a significant decrease in larval path-length and recessive lethal mutations on chromosome 3. We refer to these as reverted Csr (Csr(rv)) lines. Deficiencies that uncovered cytologically visible chromosome rearrangements in three of the seven reverted lines were used in a complementation analysis. In this way we mapped the lethal mutations in the Csr(rv) lines to cytological region 95F7-96A1 on the right arm of chromosome 3.
Full Text
The Full Text of this article is available as a PDF (810.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benzer S. BEHAVIORAL MUTANTS OF Drosophila ISOLATED BY COUNTERCURRENT DISTRIBUTION. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1112–1119. doi: 10.1073/pnas.58.3.1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benzer S. Genetic dissection of behavior. Sci Am. 1973 Dec;229(6):24–37. doi: 10.1038/scientificamerican1273-24. [DOI] [PubMed] [Google Scholar]
- Hall J. C. The mating of a fly. Science. 1994 Jun 17;264(5166):1702–1714. doi: 10.1126/science.8209251. [DOI] [PubMed] [Google Scholar]
- Nagle K. J., Bell W. J. Genetic control of the search tactic of Drosophila melanogaster: an ethometric analysis of rover/sitter traits in adult flies. Behav Genet. 1987 Jul;17(4):385–408. doi: 10.1007/BF01068138. [DOI] [PubMed] [Google Scholar]
- Pereira H. S., Sokolowski M. B. Mutations in the larval foraging gene affect adult locomotory behavior after feeding in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5044–5046. doi: 10.1073/pnas.90.11.5044. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sokolowski M. B. Foraging strategies of Drosophila melanogaster: a chromosomal analysis. Behav Genet. 1980 May;10(3):291–302. doi: 10.1007/BF01067774. [DOI] [PubMed] [Google Scholar]
- Sokolowski M. B., Hansell K. P. The foraging locus: behavioral tests for normal muscle movement in rover and sitter Drosophila melanogaster larvae. Genetica. 1992;85(3):205–209. doi: 10.1007/BF00132272. [DOI] [PubMed] [Google Scholar]
- Sokolowski M. B., Hansell R. I., Rotin D. Drosophila larval foraging behavior. II. Selection in the sibling species, D. melanogaster and D. simulans. Behav Genet. 1983 Mar;13(2):169–177. doi: 10.1007/BF01065665. [DOI] [PubMed] [Google Scholar]
- de Belle J. S., Hilliker A. J., Sokolowski M. B. Genetic localization of foraging (for): a major gene for larval behavior in Drosophila melanogaster. Genetics. 1989 Sep;123(1):157–163. doi: 10.1093/genetics/123.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Belle J. S., Sokolowski M. B., Hilliker A. J. Genetic analysis of the foraging microregion of Drosophila melanogaster. Genome. 1993 Feb;36(1):94–101. doi: 10.1139/g93-013. [DOI] [PubMed] [Google Scholar]