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. 1995 Oct;141(2):491–501. doi: 10.1093/genetics/141.2.491

Mutations in the Myp1 Gene of Ustilago Maydis Attenuate Mycelial Growth and Virulence

L Giasson 1, J W Kronstad 1
PMCID: PMC1206750  PMID: 8647387

Abstract

Mating between haploid, budding cells of the dimorphic fungus Ustilago maydis results in the formation of a dikaryotic, filamentous cell type. Mating compatibility is governed by two mating-type loci called a and b; transformation of genes from these loci (e.g., a1 and b1) into a haploid strain of different mating type (e.g., a2 b2) allows filamentous growth and establishes a pathogenic cell type. Several mutants with a nonmycelial colony morphology were isolated after insertional mutagenesis of a filamentous, pathogenic haploid strain. The mutagenized region in one such mutant was recovered by plasmid rescue and employed to isolate a gene involved in conditioning the mycelial phenotype (myp1). An 1150 amino acid open reading frame is present at the myp1 locus; the predicted polypeptide is rich in serine residues and contains short regions with similarity to SH3 domain ligands. Construction of myp1 disruption and deletion mutants in haploid strains confirmed that this gene plays a role in mycelial growth and virulence.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Bakkeren G., Kronstad J. W. Conservation of the b mating-type gene complex among bipolar and tetrapolar smut fungi. Plant Cell. 1993 Jan;5(1):123–136. doi: 10.1105/tpc.5.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Banuett F., Herskowitz I. Identification of fuz7, a Ustilago maydis MEK/MAPKK homolog required for a-locus-dependent and -independent steps in the fungal life cycle. Genes Dev. 1994 Jun 15;8(12):1367–1378. doi: 10.1101/gad.8.12.1367. [DOI] [PubMed] [Google Scholar]
  4. Banuett F. Identification of genes governing filamentous growth and tumor induction by the plant pathogen Ustilago maydis. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3922–3926. doi: 10.1073/pnas.88.9.3922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barrett K. J., Gold S. E., Kronstad J. W. Identification and complementation of a mutation to constitutive filamentous growth in Ustilago maydis. Mol Plant Microbe Interact. 1993 May-Jun;6(3):274–283. doi: 10.1094/mpmi-6-274. [DOI] [PubMed] [Google Scholar]
  6. Blacketer M. J., Koehler C. M., Coats S. G., Myers A. M., Madaule P. Regulation of dimorphism in Saccharomyces cerevisiae: involvement of the novel protein kinase homolog Elm1p and protein phosphatase 2A. Mol Cell Biol. 1993 Sep;13(9):5567–5581. doi: 10.1128/mcb.13.9.5567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chenevert J., Corrado K., Bender A., Pringle J., Herskowitz I. A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains. Nature. 1992 Mar 5;356(6364):77–79. doi: 10.1038/356077a0. [DOI] [PubMed] [Google Scholar]
  8. Cutler J. E. Putative virulence factors of Candida albicans. Annu Rev Microbiol. 1991;45:187–218. doi: 10.1146/annurev.mi.45.100191.001155. [DOI] [PubMed] [Google Scholar]
  9. Day P. R., Anagnostakis S. L. Corn smut dikaryon in culture. Nat New Biol. 1971 May 5;231(18):19–20. doi: 10.1038/newbio231019a0. [DOI] [PubMed] [Google Scholar]
  10. Day P. R., Anagnostakis S. L., Puhalla J. E. Pathogenicity resulting from mutation at the b locus of Ustilago maydis. Proc Natl Acad Sci U S A. 1971 Mar;68(3):533–535. doi: 10.1073/pnas.68.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Donnelly S. F., Pocklington M. J., Pallotta D., Orr E. A proline-rich protein, verprolin, involved in cytoskeletal organization and cellular growth in the yeast Saccharomyces cerevisiae. Mol Microbiol. 1993 Nov;10(3):585–596. doi: 10.1111/j.1365-2958.1993.tb00930.x. [DOI] [PubMed] [Google Scholar]
  13. Froeliger E. H., Leong S. A. The a mating-type alleles of Ustilago maydis are idiomorphs. Gene. 1991 Apr;100:113–122. doi: 10.1016/0378-1119(91)90356-g. [DOI] [PubMed] [Google Scholar]
  14. Gillissen B., Bergemann J., Sandmann C., Schroeer B., Bölker M., Kahmann R. A two-component regulatory system for self/non-self recognition in Ustilago maydis. Cell. 1992 Feb 21;68(4):647–657. doi: 10.1016/0092-8674(92)90141-x. [DOI] [PubMed] [Google Scholar]
  15. Gimeno C. J., Fink G. R. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol Cell Biol. 1994 Mar;14(3):2100–2112. doi: 10.1128/mcb.14.3.2100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gimeno C. J., Ljungdahl P. O., Styles C. A., Fink G. R. Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell. 1992 Mar 20;68(6):1077–1090. doi: 10.1016/0092-8674(92)90079-r. [DOI] [PubMed] [Google Scholar]
  17. Gold S., Duncan G., Barrett K., Kronstad J. cAMP regulates morphogenesis in the fungal pathogen Ustilago maydis. Genes Dev. 1994 Dec 1;8(23):2805–2816. doi: 10.1101/gad.8.23.2805. [DOI] [PubMed] [Google Scholar]
  18. Keen N. T., Tamaki S., Kobayashi D., Trollinger D. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene. 1988 Oct 15;70(1):191–197. doi: 10.1016/0378-1119(88)90117-5. [DOI] [PubMed] [Google Scholar]
  19. Kronstad J. W., Leong S. A. The b mating-type locus of Ustilago maydis contains variable and constant regions. Genes Dev. 1990 Aug;4(8):1384–1395. doi: 10.1101/gad.4.8.1384. [DOI] [PubMed] [Google Scholar]
  20. Kuspa A., Loomis W. F. Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8803–8807. doi: 10.1073/pnas.89.18.8803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  22. Laity C., Giasson L., Campbell R., Kronstad J. Heterozygosity at the b mating-type locus attenuates fusion in Ustilago maydis. Curr Genet. 1995 Apr;27(5):451–459. doi: 10.1007/BF00311215. [DOI] [PubMed] [Google Scholar]
  23. Lu S., Lyngholm L., Yang G., Bronson C., Yoder O. C., Turgeon B. G. Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12649–12653. doi: 10.1073/pnas.91.26.12649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Maresca B., Kobayashi G. S. Dimorphism in Histoplasma capsulatum: a model for the study of cell differentiation in pathogenic fungi. Microbiol Rev. 1989 Jun;53(2):186–209. doi: 10.1128/mr.53.2.186-209.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Saporito-Irwin S. M., Birse C. E., Sypherd P. S., Fonzi W. A. PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis. Mol Cell Biol. 1995 Feb;15(2):601–613. doi: 10.1128/mcb.15.2.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schulz B., Banuett F., Dahl M., Schlesinger R., Schäfer W., Martin T., Herskowitz I., Kahmann R. The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif. Cell. 1990 Jan 26;60(2):295–306. doi: 10.1016/0092-8674(90)90744-y. [DOI] [PubMed] [Google Scholar]
  28. Snell V., Nurse P. Genetic analysis of cell morphogenesis in fission yeast--a role for casein kinase II in the establishment of polarized growth. EMBO J. 1994 May 1;13(9):2066–2074. doi: 10.1002/j.1460-2075.1994.tb06481.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Spellig T., Bölker M., Lottspeich F., Frank R. W., Kahmann R. Pheromones trigger filamentous growth in Ustilago maydis. EMBO J. 1994 Apr 1;13(7):1620–1627. doi: 10.1002/j.1460-2075.1994.tb06425.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tsukuda T., Carleton S., Fotheringham S., Holloman W. K. Isolation and characterization of an autonomously replicating sequence from Ustilago maydis. Mol Cell Biol. 1988 Sep;8(9):3703–3709. doi: 10.1128/mcb.8.9.3703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wang J., Holden D. W., Leong S. A. Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc Natl Acad Sci U S A. 1988 Feb;85(3):865–869. doi: 10.1073/pnas.85.3.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Yarden O., Plamann M., Ebbole D. J., Yanofsky C. cot-1, a gene required for hyphal elongation in Neurospora crassa, encodes a protein kinase. EMBO J. 1992 Jun;11(6):2159–2166. doi: 10.1002/j.1460-2075.1992.tb05275.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yee A. R., Kronstad J. W. Construction of chimeric alleles with altered specificity at the b incompatibility locus of Ustilago maydis. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):664–668. doi: 10.1073/pnas.90.2.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yu H., Chen J. K., Feng S., Dalgarno D. C., Brauer A. W., Schreiber S. L. Structural basis for the binding of proline-rich peptides to SH3 domains. Cell. 1994 Mar 11;76(5):933–945. doi: 10.1016/0092-8674(94)90367-0. [DOI] [PubMed] [Google Scholar]

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