Abstract
A molecular-marker linkage map of hexaploid wheat (Triticum aestivum L. em. Thell) provides a framework for integration with the classical genetic map and a record of the chromosomal rearrangements involved in the evolution of this crop species. We have constructed restriction fragment length polymorphism (RFLP) maps of the A-, B-, and D-genome chromosomes of homoeologous groups 4, 5, and 7 of wheat using 114 F(7) lines from a synthetic X cultivated wheat cross and clones from 10 DNA libraries. Chromosomal breakpoints for known ancestral reciprocal translocations involving these chromosomes and for a known pericentric inversion on chromosome 4A were localized by linkage and aneuploid analysis. Known genes mapped include the major vernalization genes Vrn1 and Vrn3 on chromosome arms 5AL and 5DL, the red-coleoptile gene Rc1 on 7AS, and presumptively the leaf-rust (Puccinia recondita f.sp. tritici) resistance gene Lr34 on 7DS and the kernel-hardness gene Ha on 5DS. RFLP markers previously obtained for powdery-mildew (Blumeria graminis f.sp. tritici) resistance genes Pm2 and Pm1 were localized on chromosome arms 5DS and 7AL.
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- Ahn S., Anderson J. A., Sorrells M. E., Tanksley S. D. Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet. 1993 Dec;241(5-6):483–490. doi: 10.1007/BF00279889. [DOI] [PubMed] [Google Scholar]
- Ahn S., Tanksley S. D. Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7980–7984. doi: 10.1073/pnas.90.17.7980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Causse M. A., Fulton T. M., Cho Y. G., Ahn S. N., Chunwongse J., Wu K., Xiao J., Yu Z., Ronald P. C., Harrington S. E. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics. 1994 Dec;138(4):1251–1274. doi: 10.1093/genetics/138.4.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill B. S., Kimber G. Giemsa C-banding and the evolution of wheat. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4086–4090. doi: 10.1073/pnas.71.10.4086. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haldane J B, Waddington C H. Inbreeding and Linkage. Genetics. 1931 Jul;16(4):357–374. doi: 10.1093/genetics/16.4.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haley C. S., Knott S. A. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity (Edinb) 1992 Oct;69(4):315–324. doi: 10.1038/hdy.1992.131. [DOI] [PubMed] [Google Scholar]
- Kurata N., Nagamura Y., Yamamoto K., Harushima Y., Sue N., Wu J., Antonio B. A., Shomura A., Shimizu T., Lin S. Y. A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet. 1994 Dec;8(4):365–372. doi: 10.1038/ng1294-365. [DOI] [PubMed] [Google Scholar]
- Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
- Liu Y. G., Tsunewaki K. Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet. 1991 Oct;66(5):617–633. doi: 10.1266/jjg.66.617. [DOI] [PubMed] [Google Scholar]
- Werner J. E., Endo T. R., Gill B. S. Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11307–11311. doi: 10.1073/pnas.89.23.11307. [DOI] [PMC free article] [PubMed] [Google Scholar]