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. 1965 Jun;95(3):678–682. doi: 10.1042/bj0950678

The metabolism of l-serylglycine O[35S]-sulphate in the rat

N Tudball 1, Y Noda 1,*, K S Dodgson 1
PMCID: PMC1206793  PMID: 14342502

Abstract

1. The preparation of potassium l-serylglycine O-sulphate and the corresponding 35S-labelled ester is described. 2. Intraperitoneal injection of potassium l-serylglycine O[35S]-sulphate to rats results in about 75% of the radioactivity of the dose appearing in the urine within 48hr. Almost 72% of the radioactivity recovered in the urine was in the form of inorganic [35S]sulphate. 3. Analysis of urines by paper chromatography showed the presence of unchanged l-serylglycine O[35S]-sulphate and several other unidentified 35S-labelled materials. 4. It has been established that micro-organisms of the gastrointestinal tract do not play any significant role in the production of inorganic [35S]sulphate from the injected ester. 5. l-Serylglycine O-sulphate was hydrolysed by crude dipeptidase preparations from rat kidney and intestine to yield l-serine O-sulphate and glycine as the sole products.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAYAN J., WILSON I. B. THE FREE ENERGY OF HYDROLYSIS OF SERINE PHOSPHATE AND NACETYLETHANOLAMINE PHOSPHATE AND ITS RELATION TO PHOSPHATASE ACTIVITY. Biochim Biophys Acta. 1963 Nov 8;77:446–450. doi: 10.1016/0006-3002(63)90519-5. [DOI] [PubMed] [Google Scholar]
  2. DODGSON K. S. Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 1961 Feb;78:312–319. doi: 10.1042/bj0780312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DODGSON K. S., LLOYD A. G., TUDBALL N. O-sulphate esters of L-serine, L-threonine and L-hydroxyproline. Biochem J. 1961 Apr;79:111–117. doi: 10.1042/bj0790111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DODGSON K. S., TUDBALL N. The metabolic fate of the ester sulphate group of potassium p-nitrophenyl [35S]sulphate. Biochem J. 1960 Jan;74:154–159. doi: 10.1042/bj0740154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOHLMAN C. H. The fate of the sulfate group of chondroitin sulfate after administration to rats. Acta Physiol Scand. 1956 Sep 26;37(2-3):220–234. doi: 10.1111/j.1748-1716.1956.tb01358.x. [DOI] [PubMed] [Google Scholar]
  6. Dodgson K. S., Tudball N. Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme. Biochem J. 1961 Oct;81(1):68–71. doi: 10.1042/bj0810068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LLOYD A. G., TUDBALL N., DODGSON K. S. Infrared studies on sulphate esters. III. O-Sulphate esters of alcohols, amino alcohols and hydroxylated amino acids. Biochim Biophys Acta. 1961 Sep 30;52:413–419. doi: 10.1016/0006-3002(61)90397-3. [DOI] [PubMed] [Google Scholar]
  8. Lloyd A. G. The metabolism of exogenous N-acetyl-d-glucosamine 6-O[S]-sulphate in the normal rat. Biochem J. 1961 Sep;80(3):572–578. doi: 10.1042/bj0800572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spencer B. Endogenous sulphate acceptors in rat liver. Biochem J. 1960 Nov;77(2):294–304. doi: 10.1042/bj0770294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tudball N., Noda Y., Dodgson K. S. The metabolism of glycyl-L-serine O[35-S]-sulphate in the rat. Biochem J. 1964 Feb;90(2):439–442. doi: 10.1042/bj0900439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tudball N. The metabolism of potassium l-serine O[S]-sulphate in the rat. Biochem J. 1962 Dec;85(3):456–460. doi: 10.1042/bj0850456. [DOI] [PMC free article] [PubMed] [Google Scholar]

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