Abstract
CUG, a universal leucine codon, has been reported to be read as serine in various yeast species belonging to the genus Candida. To gain a deeper insight into the origin of this deviation from the universal genetic code, we carried out a phylogenetic analysis based on the small-subunit ribosomal RNA genes from some Candida and other related Hemiascomycetes. Furthermore, we determined the phylogenetic relationships between the tRNA(Ser)CAG, responsible for the translation of CUG, from some Candida species and the other serine and leucine isoacceptor tRNAs in C. cylindracea. We demonstrate that the group of Candida showing the genetic code deviation is monophiletic and that this deviation could have been originated more than 150 million years ago. We also describe how phylogenetic analysis can be used for genetic code predictions.
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- Barrell B. G., Bankier A. T., Drouin J. A different genetic code in human mitochondria. Nature. 1979 Nov 8;282(5735):189–194. doi: 10.1038/282189a0. [DOI] [PubMed] [Google Scholar]
- Berbee M. L., Taylor J. W. Detecting morphological convergence in true fungi, using 18S rRNA gene sequence data. Biosystems. 1992;28(1-3):117–125. doi: 10.1016/0303-2647(92)90014-p. [DOI] [PubMed] [Google Scholar]
- Kawaguchi Y., Honda H., Taniguchi-Morimura J., Iwasaki S. The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature. 1989 Sep 14;341(6238):164–166. doi: 10.1038/341164a0. [DOI] [PubMed] [Google Scholar]
- Ohama T., Suzuki T., Mori M., Osawa S., Ueda T., Watanabe K., Nakase T. Non-universal decoding of the leucine codon CUG in several Candida species. Nucleic Acids Res. 1993 Aug 25;21(17):4039–4045. doi: 10.1093/nar/21.17.4039. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osawa S., Jukes T. H., Watanabe K., Muto A. Recent evidence for evolution of the genetic code. Microbiol Rev. 1992 Mar;56(1):229–264. doi: 10.1128/mr.56.1.229-264.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osawa S., Muto A., Jukes T. H., Ohama T. Evolutionary changes in the genetic code. Proc Biol Sci. 1990 Jul 23;241(1300):19–28. doi: 10.1098/rspb.1990.0060. [DOI] [PubMed] [Google Scholar]
- Saccone C., Lanave C., Pesole G., Preparata G. Influence of base composition on quantitative estimates of gene evolution. Methods Enzymol. 1990;183:570–583. doi: 10.1016/0076-6879(90)83037-a. [DOI] [PubMed] [Google Scholar]
- Sugiyama H., Ohkuma M., Masuda Y., Park S. M., Ohta A., Takagi M. In vivo evidence for non-universal usage of the codon CUG in Candida maltosa. Yeast. 1995 Jan;11(1):43–52. doi: 10.1002/yea.320110106. [DOI] [PubMed] [Google Scholar]
- Suzuki T., Ueda T., Yokogawa T., Nishikawa K., Watanabe K. Characterization of serine and leucine tRNAs in an asporogenic yeast Candida cylindracea and evolutionary implications of genes for tRNA(Ser)CAG responsible for translation of a non-universal genetic code. Nucleic Acids Res. 1994 Jan 25;22(2):115–123. doi: 10.1093/nar/22.2.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tajima F., Nei M. Estimation of evolutionary distance between nucleotide sequences. Mol Biol Evol. 1984 Apr;1(3):269–285. doi: 10.1093/oxfordjournals.molbev.a040317. [DOI] [PubMed] [Google Scholar]
- Yokogawa T., Suzuki T., Ueda T., Mori M., Ohama T., Kuchino Y., Yoshinari S., Motoki I., Nishikawa K., Osawa S. Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7408–7411. doi: 10.1073/pnas.89.16.7408. [DOI] [PMC free article] [PubMed] [Google Scholar]