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. 1965 Jul;96(1):155–158. doi: 10.1042/bj0960155

The incorporation of labelled amino sugars by Bacillus subtilis

C J Bates 1,*, C A Pasternak 1
PMCID: PMC1206916  PMID: 14343124

Abstract

1. N-Acetyl[1-14C]glucosamine (10μm–5mm) is incorporated by cells of Bacillus subtilis at a constant rate (0·2–2mμmoles/mg. dry wt./hr.). The rate of [1-14C]glucosamine (2·5μm–5mm) incorporation is proportional to the concentration; it approaches that of N-acetyl[1-14C]glucosamine at 5mm. 2. Label from N-acetyl-[1-14C]glucosamine and [1-14C]glucosamine is incorporated predominantly into the `hot-trichloroacetic acid-soluble' and `residue' fractions of cells. Acid hydrolysis of the hot-trichloroacetic acid-soluble fraction yields mainly [14C]glucosamine; hydrolysis of the residue fraction yields [14C]glucosamine and [14C]muramic acid. The label from N-[1-14C]acetylglucosamine and sodium [1-14C]acetate enters most cell fractions. Incorporation of N-[1-14C]acetylglucosamine is inhibited by the addition of unlabelled acetate. 3. Glucose competes with [1-14C]glucosamine for incorporation. N-Propionylglucosamine and N-formylglucosamine compete with N-acetyl[1-14C]glucosamine. 4. Cells pregrown on N-acetylglucosamine or glucosamine incorporate up to ten times as much N-acetyl[1-14C]glucosamine or [1-14C]glucosamine in a given time as cells pregrown on glucose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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