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. 1965 Jul;96(1):218–223. doi: 10.1042/bj0960218

The fluorimetric microdetermination of glyoxylic acid in blood, urine and bacterial extracts

P M Zarembski 1, A Hodgkinson 1
PMCID: PMC1206925  PMID: 14343135

Abstract

1. A spectrophotofluorimetric method for the determination of glyoxylic acid in biological materials is described. 2. The method is based on the reaction between glyoxylic acid and resorcinol in acid solution, a fluorescent complex being obtained on the subsequent addition of alkali. 3. The reaction was found to be sensitive and highly specific, the minimum detectable amount of glyoxylic acid being 1·35×10−8 mole. 4. The urinary excretion of glyoxylic acid by ten normal adults ranged from 1·4 to 4·7mg./24hr. Small but measurable amounts of glyoxylic acid were found in cell-free extracts of Pseudomonas oxalaticus OX1 grown on oxalic acid as a source of carbon. No glyoxylic acid was detected in human serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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