Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 Jul;96(1):262–265. doi: 10.1042/bj0960262

Alterations in the lipids of bone caused by hypervitaminosis A and D

Richard L Cruess 1, Irwin Clark 1
PMCID: PMC1206930  PMID: 14343141

Abstract

1. The total lipid, phospholipid, total and free fatty acid, free and esterified cholesterol contents of the long bones of normal, hypervitaminotic A, D and A plus D rats were determined. 2. Toxic amounts of vitamin A decreased the total fatty content, whereas toxic amounts of vitamin D increased triglycerides, esterified cholesterol and in particular the phospholipids of bone. 3. An interaction occurred between toxic amounts of vitamins A and D, which prevented, to a large extent, the alterations in bone lipids that occur in hypervitaminosis D. 4. The studies suggest an involvement of vitamin D in lipid metabolism and tend to support the idea that lipids are involved in ossification.

Full text

PDF
262

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. CLARK I., BASSETT C. A. The amelioration of hypervitaminosis D in rats with vitamin A. J Exp Med. 1962 Jan 1;115:147–156. doi: 10.1084/jem.115.1.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK I., SMITH M. R. EFFECTS OF HYPERVITAMINOSIS A AND D ON SKELETAL METABOLISM. J Biol Chem. 1964 Apr;239:1266–1271. [PubMed] [Google Scholar]
  4. DINGLE J. T., LUCY J. A., FELL H. B. Studies on the mode of action of excess of vitamin A. 1. Effect of excess of vitamin A on the metabolism and composition of embryonic chick-limb cartilage grown in organ culture. Biochem J. 1961 Jun;79:497–500. doi: 10.1042/bj0790497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DINGLE J. T. Studies on the mode of action of excess of vitamin A. 3. Release of a bound protease by the action of vitamin A. Biochem J. 1961 Jun;79:509–512. doi: 10.1042/bj0790509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FELL H. B., MELLANBY E., PELC S. R. Influence of excess vitamin A on the sulphate metabolism of bone rudiments grown in vitro. J Physiol. 1956 Oct 29;134(1):179–188. doi: 10.1113/jphysiol.1956.sp005633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. IRVING J. T. A histological stain for newly calcified tissues. Nature. 1958 Mar 8;181(4610):704–705. doi: 10.1038/181704a0. [DOI] [PubMed] [Google Scholar]
  9. IRVING J. T. Histochemical changes in the epiphyseal cartilage during rickets. Nature. 1959 Jun 20;183(4677):1734–1735. doi: 10.1038/1831734a0. [DOI] [PubMed] [Google Scholar]
  10. LEACH A. A. The lipids of ox compact bone. Biochem J. 1958 Jul;69(3):429–432. doi: 10.1042/bj0690429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NEUBERGER A. Aspects of the metabolism of glycine and of porphyrins. Biochem J. 1961 Jan;78:1–10. doi: 10.1042/bj0780001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. THOMPSON V. W., DELUCA H. F. VITAMIN D AND PHOSPHOLIPID METABOLISM. J Biol Chem. 1964 Apr;239:984–989. [PubMed] [Google Scholar]
  13. VAN HANDEL E., ZILVERSMIT D. B. Micromethod for the direct determination of serum triglycerides. J Lab Clin Med. 1957 Jul;50(1):152–157. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES