Abstract
1. Ribosomal RNA has been prepared by extracting tissues with a phenol–cresol mixture, and ribosomal RNA can be selectively precipitated with m-cresol. No rapidly labelled RNA was associated with this material. 2. However, if RNA and DNA are extracted with 4-aminosalicylate and phenol–cresol mixture and the nucleic acids precipitated, DNA, glycogen and s-RNA (transfer RNA) can be extracted with 3m-sodium acetate and in this case rapidly labelled RNA remains associated with the ribosomal RNA. 3. The ribosomal RNA is stable in the presence of concentrated salt solution and, although the secondary structure is lost by heating at 70° in 10mm-sodium acetate, it can be re-formed in the presence of 200mm-sodium acetate. 4. The 28s and 18s components have been separated and their base compositions determined.
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Selected References
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- BROWNHILL T. J., JONES A. S., STACEY M. The inactivation of ribonuclease during the isolation of ribonucleic acids and ribonucleoproteins from yeast. Biochem J. 1959 Nov;73:434–438. doi: 10.1042/bj0730434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COX R. A., ARNSTEIN H. R. THE ISOLATION, CHARACTERIZATION AND ACID-BASE PROPERTIES OF RIBONUCLEIC ACID FROM RABBIT-RETICULOCYTE RIBOSOMES. Biochem J. 1963 Dec;89:574–584. doi: 10.1042/bj0890574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
- HUPPERT J., PELMONT J. Ultracentrifuge studies of RNA degradation. Arch Biochem Biophys. 1962 Aug;98:214–223. doi: 10.1016/0003-9861(62)90175-3. [DOI] [PubMed] [Google Scholar]
- KIDSON C., KIRBY K. S., RALPH R. K. ISOLATION CHARACTERISTICS OF RAPIDLY LABELLED RNA FROM NORMAL RAT LIVER. J Mol Biol. 1963 Sep;7:312–315. doi: 10.1016/s0022-2836(63)80010-8. [DOI] [PubMed] [Google Scholar]
- KIRBY K. S. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem J. 1957 Jul;66(3):495–504. doi: 10.1042/bj0660495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]