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. 1996 Jan;142(1):173–177. doi: 10.1093/genetics/142.1.173

Germline Transformation of Drosophila Virilis Mediated by the Transposable Element Hobo

E R Lozovskaya 1, D I Nurminsky 1, D L Hartl 1, D T Sullivan 1
PMCID: PMC1206945  PMID: 8770594

Abstract

A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile G0 animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known wide-spread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.

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Selected References

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