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. 1996 Jan;142(1):259–265. doi: 10.1093/genetics/142.1.259

Inheritance of Mitochondrial DNA and Plasmids in the Ascomycetous Fungus, Epichloe Typhina

K R Chung 1, A Leuchtmann 1, C L Schardl 1
PMCID: PMC1206955  PMID: 8770603

Abstract

We analyzed the inheritance of mitochondrial DNA (mtDNA) species in matings of the grass symbiont Epichloe typhina. Eighty progeny were analyzed from a cross in which the maternal (stromal) parent possessed three linear plasmids, designated Callan-a (7.5 kb), Aubonne-a (2.1 kb) and Bergell (2.0 kb), and the paternal parent had one plasmid, Aubonne-b (2.1 kb). Maternal transmission of all plasmids was observed in 76 progeny; two progeny possessed Bergell and Callan-a, but had the maternal Aubonne-a replaced with the related paternal plasmid Aubonne-b; two progeny lacked Callan-a, but had the other two maternal plasmids. A total of 34 progeny were analyzed from four other matings, including a reciprocal pair, and in each progeny the plasmid transmission was maternal. The inheritance of mitochondrial genomes in all progeny was analyzed by profiles of restriction endonuclease-cleaved mtDNA. In most progeny the profiles closely resembled those of the maternal parents, but some progeny had nonparental mtDNA profiles that suggested recombination of mitochondrial genomes. These results indicate that the fertilized stroma of E. typhina is initially heteroplasmic, permitting parental mitochondria to fuse and their genomes to recombine.

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Selected References

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  1. An Z. Q., Siegel M. R., Hollin W., Tsai H. F., Schmidt D., Schardl C. L. Relationships among non-Acremonium sp. fungal endophytes in five grass species. Appl Environ Microbiol. 1993 May;59(5):1540–1548. doi: 10.1128/aem.59.5.1540-1548.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birky C. W., Jr, Demko C. A., Perlman P. S., Strausberg R. Uniparental inheritance of mitochondrial genes in yeast: dependence on input bias of mitochondrial DNA and preliminary investigations of the mechanism. Genetics. 1978 Aug;89(4):615–651. doi: 10.1093/genetics/89.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Byrd A. D., Schardl C. L., Songlin P. J., Mogen K. L., Siegel M. R. The beta-tubulin gene of Epichloë typhina from perennial ryegrass (Lolium perenne). Curr Genet. 1990 Nov;18(4):347–354. doi: 10.1007/BF00318216. [DOI] [PubMed] [Google Scholar]
  4. Chan B. S., Court D. A., Vierula P. J., Bertrand H. The kalilo linear senescence-inducing plasmid of Neurospora is an invertron and encodes DNA and RNA polymerases. Curr Genet. 1991 Aug;20(3):225–237. doi: 10.1007/BF00326237. [DOI] [PubMed] [Google Scholar]
  5. Erickson L., Kemble R. Paternal inheritance of mitochondria in rapeseed (Brassica napus). Mol Gen Genet. 1990 Jun;222(1):135–139. doi: 10.1007/BF00283034. [DOI] [PubMed] [Google Scholar]
  6. Fauré S., Noyer J. L., Carreel F., Horry J. P., Bakry F., Lanaud C. Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata). Curr Genet. 1994 Mar;25(3):265–269. doi: 10.1007/BF00357172. [DOI] [PubMed] [Google Scholar]
  7. Gebeyehu G., Rao P. Y., SooChan P., Simms D. A., Klevan L. Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 1987 Jun 11;15(11):4513–4534. doi: 10.1093/nar/15.11.4513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glass N. L., Vollmer S. J., Staben C., Grotelueschen J., Metzenberg R. L., Yanofsky C. DNAs of the two mating-type alleles of Neurospora crassa are highly dissimilar. Science. 1988 Jul 29;241(4865):570–573. doi: 10.1126/science.2840740. [DOI] [PubMed] [Google Scholar]
  9. Hintz W., Anderson J. B., Horgen P. A. Nuclear migration and mitochondrial inheritance in the mushroom agaricus bitorquis. Genetics. 1988 May;119(1):35–41. doi: 10.1093/genetics/119.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoeh W. R., Blakley K. H., Brown W. M. Heteroplasmy suggests limited biparental inheritance of Mytilus mitochondrial DNA. Science. 1991 Mar 22;251(5000):1488–1490. doi: 10.1126/science.1672472. [DOI] [PubMed] [Google Scholar]
  11. Kawano S., Anderson R. W., Nanba T., Kuroiwa T. Polymorphism and uniparental inheritance of mitochondrial DNA in Physarum polycephalum. J Gen Microbiol. 1987 Nov;133(11):3175–3182. doi: 10.1099/00221287-133-11-3175. [DOI] [PubMed] [Google Scholar]
  12. Kondo R., Satta Y., Matsuura E. T., Ishiwa H., Takahata N., Chigusa S. I. Incomplete maternal transmission of mitochondrial DNA in Drosophila. Genetics. 1990 Nov;126(3):657–663. doi: 10.1093/genetics/126.3.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee S. B., Taylor J. W. Uniparental inheritance and replacement of mitochondrial DNA in Neurospora tetrasperma. Genetics. 1993 Aug;134(4):1063–1075. doi: 10.1093/genetics/134.4.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lion T., Haas O. A. Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction. Anal Biochem. 1990 Aug 1;188(2):335–337. doi: 10.1016/0003-2697(90)90616-h. [DOI] [PubMed] [Google Scholar]
  15. May G., Taylor J. W. Independent transfer of mitochondrial plasmids in Neurospora crassa. Nature. 1989 May 25;339(6222):320–322. doi: 10.1038/339320a0. [DOI] [PubMed] [Google Scholar]
  16. May G., Taylor J. W. Patterns of mating and mitochondrial DNA inheritance in the agaric Basidiomycete Coprinus cinereus. Genetics. 1988 Feb;118(2):213–220. doi: 10.1093/genetics/118.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meinhardt F., Kempken F., Kämper J., Esser K. Linear plasmids among eukaryotes: fundamentals and application. Curr Genet. 1990 Feb;17(2):89–95. doi: 10.1007/BF00312851. [DOI] [PubMed] [Google Scholar]
  18. Metzenberg R. L. The role of similarity and difference in fungal mating. Genetics. 1990 Jul;125(3):457–462. doi: 10.1093/genetics/125.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mogen K. L., Siegel M. R., Schardl C. L. Linear DNA plasmids of the perennial ryegrass choke pathogen, Epichloë typhina (Clavicipitaceae). Curr Genet. 1991 Dec;20(6):519–526. doi: 10.1007/BF00334781. [DOI] [PubMed] [Google Scholar]
  20. Mogensen H. L. Exclusion of male mitochondria and plastids during syngamy in barley as a basis for maternal inheritance. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2594–2597. doi: 10.1073/pnas.85.8.2594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Piskur J. Inheritance of the yeast mitochondrial genome. Plasmid. 1994 May;31(3):229–241. doi: 10.1006/plas.1994.1025. [DOI] [PubMed] [Google Scholar]
  22. Schaap A. P., Akhavan H., Romano L. J. Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes. Clin Chem. 1989 Sep;35(9):1863–1864. [PubMed] [Google Scholar]
  23. Schardl C. L., An Z. Molecular biology and genetics of protective fungal endophytes of grasses. Genet Eng (N Y) 1993;15:191–212. doi: 10.1007/978-1-4899-1666-2_9. [DOI] [PubMed] [Google Scholar]
  24. Schardl C. L., Leuchtmann A., Tsai H. F., Collett M. A., Watt D. M., Scott D. B. Origin of a fungal symbiont of perennial ryegrass by interspecific hybridization of a mutualist with the ryegrass choke pathogen, Epichloë typhina. Genetics. 1994 Apr;136(4):1307–1317. doi: 10.1093/genetics/136.4.1307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Skibinski D. O., Gallagher C., Beynon C. M. Mitochondrial DNA inheritance. Nature. 1994 Apr 28;368(6474):817–818. doi: 10.1038/368817b0. [DOI] [PubMed] [Google Scholar]
  26. Smith M. L., Duchesne L. C., Bruhn J. N., Anderson J. B. Mitochondrial genetics in a natural population of the plant pathogen armillaria. Genetics. 1990 Nov;126(3):575–582. doi: 10.1093/genetics/126.3.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tsai H. F., Liu J. S., Staben C., Christensen M. J., Latch G. C., Siegel M. R., Schardl C. L. Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2542–2546. doi: 10.1073/pnas.91.7.2542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yang X., Griffiths A. J. Male transmission of linear plasmids and mitochondrial DNA in the fungus Neurospora. Genetics. 1993 Aug;134(4):1055–1062. doi: 10.1093/genetics/134.4.1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yang X., Griffiths A. J. Plasmid suppressors active in the sexual cycle of Neurospora intermedia. Genetics. 1993 Dec;135(4):993–1002. doi: 10.1093/genetics/135.4.993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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