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. 1996 Feb;142(2):407–416. doi: 10.1093/genetics/142.2.407

Poorly Repaired Mismatches in Heteroduplex DNA Are Hyper-Recombinagenic in Saccharomyces Cerevisiae

P Manivasakam 1, S M Rosenberg 1, P J Hastings 1
PMCID: PMC1206975  PMID: 8852840

Abstract

In yeast meiotic recombination, alleles used as genetic markers fall into two classes as regards their fate when incorporated into heteroduplex DNA. Normal alleles are those that form heteroduplexes that are nearly always recognized and corrected by the mismatch repair system operating in meiosis. High PMS (postmeiotic segregation) alleles form heteroduplexes that are inefficiently mismatch repaired. We report that placing any of several high PMS alleles very close to normal alleles causes hyperrecombination between these markers. We propose that this hyperrecombination is caused by the high PMS allele blocking a mismatch repair tract initiated from the normal allele, thus preventing corepair of the two alleles, which would prevent formation of recombinants. The results of three point crosses involving two PMS alleles and a normal allele suggest that high PMS alleles placed between two alleles that are normally corepaired block that corepair.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alani E., Reenan R. A., Kolodner R. D. Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiae. Genetics. 1994 May;137(1):19–39. doi: 10.1093/genetics/137.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carpenter A. T. Meiotic roles of crossing-over and of gene conversion. Cold Spring Harb Symp Quant Biol. 1984;49:23–29. doi: 10.1101/sqb.1984.049.01.005. [DOI] [PubMed] [Google Scholar]
  3. Detloff P., Petes T. D. Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae. Mol Cell Biol. 1992 Apr;12(4):1805–1814. doi: 10.1128/mcb.12.4.1805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Korch C. T., Snow R. Allelic Complementation in the First Gene for Histidine Biosynthesis in SACCHAROMYCES CEREVISIAE. I. Characteristics of Mutants and Genetic Mapping of Alleles. Genetics. 1973 Jun;74(2):287–305. doi: 10.1093/genetics/74.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Malone R. E., Bullard S., Lundquist S., Kim S., Tarkowski T. A meiotic gene conversion gradient opposite to the direction of transcription. Nature. 1992 Sep 10;359(6391):154–155. doi: 10.1038/359154a0. [DOI] [PubMed] [Google Scholar]
  7. Modrich P. Mechanisms and biological effects of mismatch repair. Annu Rev Genet. 1991;25:229–253. doi: 10.1146/annurev.ge.25.120191.001305. [DOI] [PubMed] [Google Scholar]
  8. Moore C. W., Hampsey D. M., Ernst J. F., Sherman F. Differential mismatch repair can explain the disproportionalities between physical distances and recombination frequencies of cyc1 mutations in yeast. Genetics. 1988 May;119(1):21–34. doi: 10.1093/genetics/119.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muster-Nassal C., Kolodner R. Mismatch correction catalyzed by cell-free extracts of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7618–7622. doi: 10.1073/pnas.83.20.7618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nag D. K., White M. A., Petes T. D. Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast. Nature. 1989 Jul 27;340(6231):318–320. doi: 10.1038/340318a0. [DOI] [PubMed] [Google Scholar]
  11. Prolla T. A., Pang Q., Alani E., Kolodner R. D., Liskay R. M. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994 Aug 19;265(5175):1091–1093. doi: 10.1126/science.8066446. [DOI] [PubMed] [Google Scholar]
  12. Reenan R. A., Kolodner R. D. Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics. 1992 Dec;132(4):963–973. doi: 10.1093/genetics/132.4.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rose M. D., Novick P., Thomas J. H., Botstein D., Fink G. R. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene. 1987;60(2-3):237–243. doi: 10.1016/0378-1119(87)90232-0. [DOI] [PubMed] [Google Scholar]
  14. Ross-Macdonald P., Roeder G. S. Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction. Cell. 1994 Dec 16;79(6):1069–1080. doi: 10.1016/0092-8674(94)90037-x. [DOI] [PubMed] [Google Scholar]
  15. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  16. Schär P., Kohli J. Marker effects of G to C transversions on intragenic recombination and mismatch repair in Schizosaccharomyces pombe. Genetics. 1993 Apr;133(4):825–835. doi: 10.1093/genetics/133.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White J. H., DiMartino J. F., Anderson R. W., Lusnak K., Hilbert D., Fogel S. A DNA sequence conferring high postmeiotic segregation frequency to heterozygous deletions in Saccharomyces cerevisiae is related to sequences associated with eucaryotic recombination hotspots. Mol Cell Biol. 1988 Mar;8(3):1253–1258. doi: 10.1128/mcb.8.3.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]

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