Abstract
Direct selection for increased resistance to a heat shock (41.9° for 90 min) was carried out using two replicate lines of Drosophila buzzatii that were derived from a large base population. Selected individuals were first acclimated to high temperature before selection, while control individuals were acclimated but not selected, and selection was performed every second generation. Resistance to heat shock with acclimation increased in selected lines. Without acclimation, a correlated smaller increase in heat-shock resistance was suggested. Survival of males was higher than that of females in all lines when tested with acclimation, but with direct exposure to high temperatures, survival of females was greater than that of males both in selection and control lines but not in the base population. From analysis of reciprocal cross progeny between lines, one selection line was found to possess a dominant autosomal factor that significantly increased resistance of males much more than resistance of females. Also suggestive was recessive traits on the X chromosome in both selection lines that increased thermotolerance. No cytoplasmic effects were found. After accounting for other effects, survival of F(1) flies was intermediate, suggesting that additive variation is present for one or more of the autosomes.
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Selected References
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- Ashburner M., Bonner J. J. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. doi: 10.1016/0092-8674(79)90150-8. [DOI] [PubMed] [Google Scholar]
- Dahlgaard J., Krebs R. A., Loeschcke V. Heat-shock tolerance and inbreeding in Drosophila buzzatii. Heredity (Edinb) 1995 Feb;74(Pt 2):157–163. doi: 10.1038/hdy.1995.23. [DOI] [PubMed] [Google Scholar]
- DiDomenico B. J., Bugaisky G. E., Lindquist S. Heat shock and recovery are mediated by different translational mechanisms. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6181–6185. doi: 10.1073/pnas.79.20.6181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiDomenico B. J., Bugaisky G. E., Lindquist S. The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels. Cell. 1982 Dec;31(3 Pt 2):593–603. doi: 10.1016/0092-8674(82)90315-4. [DOI] [PubMed] [Google Scholar]
- Fisher B., Kraft P., Hahn G. M., Anderson R. L. Thermotolerance in the absence of induced heat shock proteins in a murine lymphoma. Cancer Res. 1992 May 15;52(10):2854–2861. [PubMed] [Google Scholar]
- Hosgood S. M., Parsons P. A. Polymorphism in natural populations of Drosophila for the ability to withstand temperature shocks. Experientia. 1968 Jul 15;24(7):727–728. doi: 10.1007/BF02138343. [DOI] [PubMed] [Google Scholar]
- Jenkins N. L., Hoffmann A. A. Genetic and maternal variation for heat resistance in Drosophila from the field. Genetics. 1994 Jul;137(3):783–789. doi: 10.1093/genetics/137.3.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
- Morrison W. W., Milkman R. Modification of heat resistance in Drosophila by selection. Nature. 1978 May 4;273(5657):49–50. doi: 10.1038/273049b0. [DOI] [PubMed] [Google Scholar]
- Oudman L. A locus in Drosophila melanogaster affecting heat resistance. Hereditas. 1991;114(3):285–287. doi: 10.1111/j.1601-5223.1991.tb00337.x. [DOI] [PubMed] [Google Scholar]
- Parker-Thornburg J., Bonner J. J. Mutations that induce the heat shock response of Drosophila. Cell. 1987 Dec 4;51(5):763–772. doi: 10.1016/0092-8674(87)90099-7. [DOI] [PubMed] [Google Scholar]