Abstract
1. Purified horse-liver alcohol dehydrogenase is heterogeneous on starch-gel electrophoresis in several buffer systems. 2. The electrophoretic pattern is altered by the addition to the buffers of oxidized or reduced coenzymes, isobutyramide, metal ions or metal-chelating agents. 3. The effect of coenzymes on the pattern suggests that the major cause of the observed heterogeneity is not the existence of isoenzymes, but the presence in the enzyme preparations of coenzyme–enzyme complexes or complexes with other nucleotides similar to, but less reactive than, the coenzymes. 4. Metal ions and chelating agents influence the electrophoretic separation by partial denaturation and inactivation of the enzyme.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- APPELLA E., MARKERT C. L. Dissociation of lactate dehydrogenase into subunits with guanidine hydrochloride. Biochem Biophys Res Commun. 1961 Nov 20;6:171–176. doi: 10.1016/0006-291x(61)90123-1. [DOI] [PubMed] [Google Scholar]
- Cahn R. D., Zwilling E., Kaplan N. O., Levine L. Nature and Development of Lactic Dehydrogenases: The two major types of this enzyme form molecular hybrids which change in makeup during development. Science. 1962 Jun 15;136(3520):962–969. doi: 10.1126/science.136.3520.962. [DOI] [PubMed] [Google Scholar]
- DALZIEL K. The preparation and properties of crystalline alcohol dehydrogenase from liver. Biochem J. 1961 Aug;80:440–445. doi: 10.1042/bj0800440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRITZ P. J., JACOBSON K. B. Lactic dehydrogenases: subfractionation of isozymes. Science. 1963 Apr 5;140(3562):64–65. doi: 10.1126/science.140.3562.64. [DOI] [PubMed] [Google Scholar]
- SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATTS D. C., DONNINGER C. Starch gel electrophoresis as a criterion for the purity of crystalline yeast alcohol dehydrogenase. Anal Biochem. 1962 Jun;3:489–497. doi: 10.1016/0003-2697(62)90082-9. [DOI] [PubMed] [Google Scholar]
- WIELAND T., PFLEIDERER G. MULTIPLE FORMEN VON ENZYMEN. Adv Enzymol Relat Areas Mol Biol. 1963;25:329–368. [PubMed] [Google Scholar]