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. 1965 Sep;96(3):626–633. doi: 10.1042/bj0960626

High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins

T C Hall 1, E C Cocking 1
PMCID: PMC1207196  PMID: 5862403

Abstract

1. Inexpensive scintillation mixtures are described which enable the detection of as little as 40μμc of 14C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of 14C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.

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Selected References

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