Skip to main content
. 2025 May 3;26(9):4351. doi: 10.3390/ijms26094351
3Rs Replacement, Reduction, and Refinement (principios éticos en investigación animal)
ALP Alkaline Phosphatase
AP Alkaline Phosphatase
ATAC-seq Assay for Transposase-Accessible Chromatin using sequencing
ATCC American Type Culture Collection
bFGF Basic Fibroblast Growth Factor
CD24 Cluster of Differentiation 24
CMV Cytomegalovirus (viral promoter)
CRISPR Clustered Regularly Interspaced Short Palindromic Repeats
CRISPRa/i CRISPR activation/interference
ctgfa Connective Tissue Growth Factor a
cyp1a1 Cytochrome P450 1A1
DMEM Dulbecco’s Modified Eagle Medium
DMEM/F12 DMEM supplemented with Ham’s F12 Nutrient Mix
DRCF Danio rerio Caudal Fin cell line
ECACC European Collection of Authenticated Cell Cultures
ESC Embryonic Stem Cell
FBS Fetal Bovine Serum
fli1 Friend Leukemia Virus Integration 1
G418 Geneticin (aminoglycoside antibiotic)
gdf3 Growth Differentiation Factor 3
GFP Green Fluorescent Protein
HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (buffering agent)
hESC Human Embryonic Stem Cell
hpf Hours post-fertilization
kdr Kinase Insert Domain Receptor (VEGFR2)
klf4 Kruppel-like Factor 4
KO Knockout
L-15 Leibovitz’s L-15 Medium
lin28 LIN28 Homolog A
MPC 2-methacryloxyloxyethyl phosphorylcholine
nanog Homeobox protein Nanog
oct4 Octamer-binding transcription factor 4 (pou5f1)
PAC2 Zebrafish embryonic fibroblast cell line derived from 24 hpf embryos
PCR Polymerase Chain Reaction
pEGFP-N1 Plasmid expressing Enhanced Green Fluorescent Protein under CMV promoter
PGC Primordial Germ Cell
pou5f1 POU domain class 5 transcription factor 1
ronin Required for Nuclear Factor Activation in Stem Cells
RSV Rous Sarcoma Virus
RTS34st Rainbow Trout Spleen Stromal Cell Line
sall4 Spalt Like Transcription Factor 4
SDF-1b Stromal Cell-Derived Factor 1 beta
sox2 SRY-box Transcription Factor 2
SSEA Stage-Specific Embryonic Antigen
STR Short Tandem Repeat
TCDD 2,3,7,8-Tetrachlorodibenzo-p-dioxin
tcf3 Transcription Factor 3
tdgf1 Teratocarcinoma-Derived Growth Factor 1
Tol2 Transposon originally from Oryzias latipes
TRA-1-60/81 Tumor-Related Antigens
vasa DEAD-box Helicase 4 (germ cell marker)
Z428 Zebrafish embryonic stem-like cell line
ZBE3 Zebrafish Blastula-derived Embryonic cell line with high transfection efficiency
ZEB2J Zebrafish Embryonic Blastula-derived cell line adapted from co-culture
ZEM-2
ZEM-2A
ZEM2S
Zebrafish Embryonic cell lines with varying serum/media conditions
ZES1 Zebrafish embryonic stem-like cell line
ZF4 Zebrafish Fibroblast cell line
ZFL Zebrafish Liver cell line
zPDX Zebrafish Patient-Derived Xenograft