Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1996 Jun;143(2):811–821. doi: 10.1093/genetics/143.2.811

Identification, Mapping and Linkage Analysis of Randomly Amplified DNA Polymorphisms in Tetrahymena Thermophila

J H Brickner 1, T J Lynch 1, D Zeilinger 1, E Orias 1
PMCID: PMC1207339  PMID: 8725229

Abstract

Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from C3-368-5 (C3 strain). Polymorphisms were mapped to chromosomes using a panel of monosomic strains constructed by crossing B strain-derived nullisomic strains to inbred strain C3. They map to all five chromosomes and appear to be evenly distributed throughout the genome. Chromosomal groups were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chromosomes 1R, 2L, 3 and 5. The RAPD method appears useful for the construction of a genetic map of the Tetrahymena genome based on DNA polymorphisms.

Full Text

The Full Text of this article is available as a PDF (3.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN S. L. LINKAGE STUDIES IN VARIETY 1 OF TETRAHYMENA PYRIFORMIS: A FIRST CASE OF LINKAGE IN THE CILIATED PROTOZOA. Genetics. 1964 Apr;49:617–627. doi: 10.1093/genetics/49.4.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen S. L. Cytogenetics of genomic exclusion in Tetrahymena. Genetics. 1967 Apr;55(4):797–822. doi: 10.1093/genetics/55.4.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen S. L., Ervin P. R., McLaren N. C., Brand R. E. The 5S ribosomal RNA gene clusters in Tetrahymena thermophila: strain differences, chromosomal localization, and loss during micronuclear ageing. Mol Gen Genet. 1984;197(2):244–253. doi: 10.1007/BF00330970. [DOI] [PubMed] [Google Scholar]
  4. Allen S. L. Genomic exclusion: a rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, syngen 1. Science. 1967 Feb 3;155(3762):575–577. doi: 10.1126/science.155.3762.575. [DOI] [PubMed] [Google Scholar]
  5. Bleyman L. K., Baum M. P., Bruns P. J., Orias E. Mapping the mating type locus of Tetrahymena thermophila: meiotic linkage of mat to the ribosomal RNA gene. Dev Genet. 1992;13(1):34–40. doi: 10.1002/dvg.1020130106. [DOI] [PubMed] [Google Scholar]
  6. Bruns P. J., Brussard T. B., Merriam E. V. Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes. Genetics. 1983 Jun;104(2):257–270. doi: 10.1093/genetics/104.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bruns P. J., Brussard T. B. Positive selection for mating with functional heterokaryons in Tetrahymena pyriformis. Genetics. 1974 Nov;78(3):831–841. doi: 10.1093/genetics/78.3.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cassidy-Hanley D., Yao M. C., Bruns P. J. A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction. Genetics. 1994 May;137(1):95–106. doi: 10.1093/genetics/137.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gaertig J., Gorovsky M. A. Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9196–9200. doi: 10.1073/pnas.89.19.9196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gomez S., di Bello C., Lam T. H., Genet R., Morgat J. L., Fromageot P., Cohen P. C-terminal amidation of neuropeptides. Gly-Lys-Arg extension an efficient precursor of C-terminal amide. FEBS Lett. 1984 Feb 13;167(1):160–164. doi: 10.1016/0014-5793(84)80853-4. [DOI] [PubMed] [Google Scholar]
  11. Hamilton E. P., Suhr-Jessen P. B., Orias E. Pronuclear fusion failure: an alternate conjugational pathway in Tetrahymena thermophila, induced by vinblastine. Genetics. 1988 Apr;118(4):627–636. doi: 10.1093/genetics/118.4.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kruger K., Grabowski P. J., Zaug A. J., Sands J., Gottschling D. E., Cech T. R. Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell. 1982 Nov;31(1):147–157. doi: 10.1016/0092-8674(82)90414-7. [DOI] [PubMed] [Google Scholar]
  13. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  14. Larson D. D., Blackburn E. H., Yaeger P. C., Orias E. Control of rDNA replication in Tetrahymena involves a cis-acting upstream repeat of a promoter element. Cell. 1986 Oct 24;47(2):229–240. doi: 10.1016/0092-8674(86)90445-9. [DOI] [PubMed] [Google Scholar]
  15. Luehrsen K. R., Baum M. P., Orias E. A restriction fragment length polymorphism in the 5' non-transcribed spacer of the rDNA of Tetrahymena thermophila inbred strains B and C3. Gene. 1987;55(2-3):169–178. doi: 10.1016/0378-1119(87)90277-0. [DOI] [PubMed] [Google Scholar]
  16. Lynch T. J., Brickner J., Nakano K. J., Orias E. Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila. Genetics. 1995 Dec;141(4):1315–1325. doi: 10.1093/genetics/141.4.1315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McCoy J. W. Linkage and Genetic Map Length in TETRAHYMENA THERMOPHILA. Genetics. 1977 Nov;87(3):421–439. doi: 10.1093/genetics/87.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Raikov I. B. Evolution of macronuclear organization. Annu Rev Genet. 1976;10:413–440. doi: 10.1146/annurev.ge.10.120176.002213. [DOI] [PubMed] [Google Scholar]
  19. Tingey S. V., del Tufo J. P. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol. 1993 Feb;101(2):349–352. doi: 10.1104/pp.101.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yu G. L., Bradley J. D., Attardi L. D., Blackburn E. H. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs. Nature. 1990 Mar 8;344(6262):126–132. doi: 10.1038/344126a0. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES