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. 1996 Jul;143(3):1321–1337. doi: 10.1093/genetics/143.3.1321

Scalloped Wings Is the Lucilia Cuprina Notch Homologue and a Candidate for the Modifier of Fitness and Asymmetry of Diazinon Resistance

A G Davies 1, A Y Game 1, Z Chen 1, T J Williams 1, S Goodall 1, J L Yen 1, J A McKenzie 1, P Batterham 1
PMCID: PMC1207401  PMID: 8807304

Abstract

The Scalloped wings (Scl) gene of the Australian sheep blowfly, Lucilia cuprina, is shown to be the homologue of the Drosophila melanogaster Notch gene by comparison at the DNA sequence and genetic levels. A L. cuprina genomic fragment, which shows strong identity with the Notch (N) gene at the molecular level, hybridizes to the location of the Scl gene on polytene chromosomes. The two genes are functionally homologous; the dominant and recessive Notch-like phenotypes produced by mutations in the Scl gene allow these alleles to be classed as N-like or Abruptex-like. The Scl gene is under investigation as a candidate for the fitness and asymmetry Modifier (M) of diazinon resistance. We show that M affects the penetrance of wing and bristle phenotypes associated with two Scl alleles in a manner consistent with the M being an allele of Scl. In addition, we report a phenotypic interaction between the diazinon-resistance mutation, Rop-1, and the same alleles of Scl. We propose that the product of Rop-1, an esterase, may be involved in cell adhesion in developmental processes involving the Scl gene product.

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Selected References

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  1. Bate M., Rushton E., Frasch M. A dual requirement for neurogenic genes in Drosophila myogenesis. Dev Suppl. 1993:149–161. [PubMed] [Google Scholar]
  2. Bedo D. G. Differential sex chromosome replication and dosage compensation in polytene trichogen cells of Lucilia cuprina (Diptera: Calliphoridae). Chromosoma. 1982;87(1):21–32. doi: 10.1007/BF00333507. [DOI] [PubMed] [Google Scholar]
  3. Cagan R. L., Ready D. F. Notch is required for successive cell decisions in the developing Drosophila retina. Genes Dev. 1989 Aug;3(8):1099–1112. doi: 10.1101/gad.3.8.1099. [DOI] [PubMed] [Google Scholar]
  4. Fortini M. E., Artavanis-Tsakonas S. Notch: neurogenesis is only part of the picture. Cell. 1993 Dec 31;75(7):1245–1247. doi: 10.1016/0092-8674(93)90611-s. [DOI] [PubMed] [Google Scholar]
  5. Fortini M. E., Rebay I., Caron L. A., Artavanis-Tsakonas S. An activated Notch receptor blocks cell-fate commitment in the developing Drosophila eye. Nature. 1993 Oct 7;365(6446):555–557. doi: 10.1038/365555a0. [DOI] [PubMed] [Google Scholar]
  6. Foster G. G. Negative complementation at the notch locus of Drosophila melanogaster. Genetics. 1975 Sep;81(1):99–120. doi: 10.1093/genetics/81.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hortsch M., Goodman C. S. Cell and substrate adhesion molecules in Drosophila. Annu Rev Cell Biol. 1991;7:505–557. doi: 10.1146/annurev.cb.07.110191.002445. [DOI] [PubMed] [Google Scholar]
  8. Hortsch M., Patel N. H., Bieber A. J., Traquina Z. R., Goodman C. S. Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis. Development. 1990 Dec;110(4):1327–1340. doi: 10.1242/dev.110.4.1327. [DOI] [PubMed] [Google Scholar]
  9. Jan L. Y., Jan Y. N. Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and in grasshopper embryos. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2700–2704. doi: 10.1073/pnas.79.8.2700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kidd S., Kelley M. R., Young M. W. Sequence of the notch locus of Drosophila melanogaster: relationship of the encoded protein to mammalian clotting and growth factors. Mol Cell Biol. 1986 Sep;6(9):3094–3108. doi: 10.1128/mcb.6.9.3094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lieber T., Kidd S., Alcamo E., Corbin V., Young M. W. Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei. Genes Dev. 1993 Oct;7(10):1949–1965. doi: 10.1101/gad.7.10.1949. [DOI] [PubMed] [Google Scholar]
  12. Lieber T., Wesley C. S., Alcamo E., Hassel B., Krane J. F., Campos-Ortega J. A., Young M. W. Single amino acid substitutions in EGF-like elements of Notch and Delta modify Drosophila development and affect cell adhesion in vitro. Neuron. 1992 Nov;9(5):847–859. doi: 10.1016/0896-6273(92)90238-9. [DOI] [PubMed] [Google Scholar]
  13. Lohs-Schardin M., Cremer C., Nüsslein-Volhard C. A fate map for the larval epidermis of Drosophila melanogaster: localized cuticle defects following irradiation of the blastoderm with an ultraviolet laser microbeam. Dev Biol. 1979 Dec;73(2):239–255. doi: 10.1016/0012-1606(79)90065-4. [DOI] [PubMed] [Google Scholar]
  14. McKenzie J. A., Clarke G. M. Diazinon resistance, fluctuating asymmetry and fitness in the Australian sheep blowfly, lucilia cuprina. Genetics. 1988 Sep;120(1):213–220. doi: 10.1093/genetics/120.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Muskavitch M. A. Delta-notch signaling and Drosophila cell fate choice. Dev Biol. 1994 Dec;166(2):415–430. doi: 10.1006/dbio.1994.1326. [DOI] [PubMed] [Google Scholar]
  16. Olson P. F., Fessler L. I., Nelson R. E., Sterne R. E., Campbell A. G., Fessler J. H. Glutactin, a novel Drosophila basement membrane-related glycoprotein with sequence similarity to serine esterases. EMBO J. 1990 Apr;9(4):1219–1227. doi: 10.1002/j.1460-2075.1990.tb08229.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Portin P. Allelic negative complementation at the Abruptex locus of Drosophila melanogaster. Genetics. 1975 Sep;81(1):121–133. doi: 10.1093/genetics/81.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rebay I., Fehon R. G., Artavanis-Tsakonas S. Specific truncations of Drosophila Notch define dominant activated and dominant negative forms of the receptor. Cell. 1993 Jul 30;74(2):319–329. doi: 10.1016/0092-8674(93)90423-n. [DOI] [PubMed] [Google Scholar]
  19. Rebay I., Fleming R. J., Fehon R. G., Cherbas L., Cherbas P., Artavanis-Tsakonas S. Specific EGF repeats of Notch mediate interactions with Delta and Serrate: implications for Notch as a multifunctional receptor. Cell. 1991 Nov 15;67(4):687–699. doi: 10.1016/0092-8674(91)90064-6. [DOI] [PubMed] [Google Scholar]
  20. Roush R. T., McKenzie J. A. Ecological genetics of insecticide and acaricide resistance. Annu Rev Entomol. 1987;32:361–380. doi: 10.1146/annurev.en.32.010187.002045. [DOI] [PubMed] [Google Scholar]
  21. Ruohola H., Bremer K. A., Baker D., Swedlow J. R., Jan L. Y., Jan Y. N. Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila. Cell. 1991 Aug 9;66(3):433–449. doi: 10.1016/0092-8674(81)90008-8. [DOI] [PubMed] [Google Scholar]
  22. Shellenbarger D. L., Mohler J. D. Temperature-sensitive mutations of the notch locus in Drosophila melanogaster. Genetics. 1975 Sep;81(1):143–162. doi: 10.1093/genetics/81.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smyth K. A., Parker A. G., Yen J. L., McKenzie J. A. Selection of dieldrin-resistant strains of Lucilia cuprina (Diptera: Calliphoridae) after ethyl methanesulfonate mutagenesis of a susceptible strain. J Econ Entomol. 1992 Apr;85(2):352–358. doi: 10.1093/jee/85.2.352. [DOI] [PubMed] [Google Scholar]
  24. Snow P. M., Patel N. H., Harrelson A. L., Goodman C. S. Neural-specific carbohydrate moiety shared by many surface glycoproteins in Drosophila and grasshopper embryos. J Neurosci. 1987 Dec;7(12):4137–4144. doi: 10.1523/JNEUROSCI.07-12-04137.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tautz D., Pfeifle C. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma. 1989 Aug;98(2):81–85. doi: 10.1007/BF00291041. [DOI] [PubMed] [Google Scholar]
  26. Weller G. L., Foster G. G. Genetic maps of the sheep blowfly Lucilia cuprina: linkage-group correlations with other dipteran genera. Genome. 1993 Jun;36(3):495–506. doi: 10.1139/g93-068. [DOI] [PubMed] [Google Scholar]
  27. Wharton K. A., Johansen K. M., Xu T., Artavanis-Tsakonas S. Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats. Cell. 1985 Dec;43(3 Pt 2):567–581. doi: 10.1016/0092-8674(85)90229-6. [DOI] [PubMed] [Google Scholar]
  28. Xu T., Caron L. A., Fehon R. G., Artavanis-Tsakonas S. The involvement of the Notch locus in Drosophila oogenesis. Development. 1992 Aug;115(4):913–922. doi: 10.1242/dev.115.4.913. [DOI] [PubMed] [Google Scholar]
  29. de Celis J. F., Garcia-Bellido A. Modifications of the notch function by Abruptex mutations in Drosophila melanogaster. Genetics. 1994 Jan;136(1):183–194. doi: 10.1093/genetics/136.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]

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