Abstract
This study examines the use of neutral genetic markers to guide sampling from a large germplasm collection with the objective of establishing from it a smaller, but genetically representative sample. We simulated evolutionary change and germplasm sampling in a subdivided population of a diploid hermaphrodite annual plant to create an initially large collection. Several strategies of sampling from this collection were then compared. Our results show that a strategy based on information obtained from marker genes led to retention of the maximum number of neutral and nonneutral alleles in the smaller sample. This occurred when demes were composed of self-fertilizing individuals or when no migration occurred among demes, but not when demes of an outcrossing population were connected by high levels of migration.
Full Text
The Full Text of this article is available as a PDF (901.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown A. H., Clegg M. T. Isozyme assessment of plant genetic resources. Isozymes Curr Top Biol Med Res. 1983;11:285–295. [PubMed] [Google Scholar]
- Ewens W. J. The sampling theory of selectively neutral alleles. Theor Popul Biol. 1972 Mar;3(1):87–112. doi: 10.1016/0040-5809(72)90035-4. [DOI] [PubMed] [Google Scholar]
- Henderson S. T., Petes T. D. Instability of simple sequence DNA in Saccharomyces cerevisiae. Mol Cell Biol. 1992 Jun;12(6):2749–2757. doi: 10.1128/mcb.12.6.2749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hospital F., Chevalet C. Interactions of selection, linkage and drift in the dynamics of polygenic characters. Genet Res. 1996 Feb;67(1):77–87. doi: 10.1017/s0016672300033498. [DOI] [PubMed] [Google Scholar]
- Schoen D. J., Brown A. H. Conservation of allelic richness in wild crop relatives is aided by assessment of genetic markers. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10623–10627. doi: 10.1073/pnas.90.22.10623. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slatkin M. Inbreeding coefficients and coalescence times. Genet Res. 1991 Oct;58(2):167–175. doi: 10.1017/s0016672300029827. [DOI] [PubMed] [Google Scholar]