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. 1996 Sep;144(1):87–94. doi: 10.1093/genetics/144.1.87

Multiple Versions of the a Mating Type Locus of Coprinus Cinereus Are Generated by Three Paralogous Pairs of Multiallelic Homeobox Genes

E H Pardo 1, S F O'Shea 1, L A Casselton 1
PMCID: PMC1207520  PMID: 8878675

Abstract

The A mating type locus of Coprinus cinereus determines mating compatibility by regulating essential steps in sexual development. Each A locus contains several genes separated into two functionally independent complexes termed Aα and Aβ, and the multiple alleles of these genes generate an estimated 160 A mating specificities. The genes encode two classes of homeodomain-containing proteins designated HD1 and HD2. In this report we describe two newly cloned loci, A2 and A5, and compare them with A42, A43 and A6 that we have described previously. An Aβ-null locus, retaining just a single active HD1 gene from the α-complex, was generated by mutation. Using this as a transformation host, gene combinations that promote A-regulated development were identified. We demonstrate that each A locus contains members of three paralogous pairs of HD1 and HD2 genes. Different allelic versions of gene pairs are compatible but paralogous genes are incompatible. The genes present in four uncloned A loci were deduced using Southern analyses and transformations with available cloned genes. The combined analysis of nine A factors identifies sufficient A gene alleles to generate at least 72 A mating specificities.

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Selected References

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  1. Banham A. H., Asante-Owusu R. N., Gottgens B., Thompson S., Kingsnorth C. S., Mellor E., Casselton L. A. An N-Terminal Dimerization Domain Permits Homeodomain Proteins To Choose Compatible Partners and Initiate Sexual Development in the Mushroom Coprinus cinereus. Plant Cell. 1995 Jun;7(6):773–783. doi: 10.1105/tpc.7.6.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binninger D. M., Skrzynia C., Pukkila P. J., Casselton L. A. DNA-mediated transformation of the basidiomycete Coprinus cinereus. EMBO J. 1987 Apr;6(4):835–840. doi: 10.1002/j.1460-2075.1987.tb04828.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Day P R. The Structure of the a Mating Type Locus in Coprinus Lagopus. Genetics. 1960 May;45(5):641–650. doi: 10.1093/genetics/45.5.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gieser P. T., May G. Comparison of two b1 alleles from within the A mating-type of the basidiomycete Coprinus cinereus. Gene. 1994 Sep 2;146(2):167–176. doi: 10.1016/0378-1119(94)90289-5. [DOI] [PubMed] [Google Scholar]
  5. Gillissen B., Bergemann J., Sandmann C., Schroeer B., Bölker M., Kahmann R. A two-component regulatory system for self/non-self recognition in Ustilago maydis. Cell. 1992 Feb 21;68(4):647–657. doi: 10.1016/0092-8674(92)90141-x. [DOI] [PubMed] [Google Scholar]
  6. Kües U., Asante-Owusu R. N., Mutasa E. S., Tymon A. M., Pardo E. H., O'Shea S. F., Göttgens B., Casselton L. A. Two classes of homeodomain proteins specify the multiple a mating types of the mushroom Coprinus cinereus. Plant Cell. 1994 Oct;6(10):1467–1475. doi: 10.1105/tpc.6.10.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kües U., Asante-Owusu R. N., Mutasa E. S., Tymon A. M., Pardo E. H., O'Shea S. F., Göttgens B., Casselton L. A. Two classes of homeodomain proteins specify the multiple a mating types of the mushroom Coprinus cinereus. Plant Cell. 1994 Oct;6(10):1467–1475. doi: 10.1105/tpc.6.10.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kües U., Göttgens B., Stratmann R., Richardson W. V., O'Shea S. F., Casselton L. A. A chimeric homeodomain protein causes self-compatibility and constitutive sexual development in the mushroom Coprinus cinereus. EMBO J. 1994 Sep 1;13(17):4054–4059. doi: 10.1002/j.1460-2075.1994.tb06722.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kües U., Richardson W. V., Tymon A. M., Mutasa E. S., Göttgens B., Gaubatz S., Gregoriades A., Casselton L. A. The combination of dissimilar alleles of the A alpha and A beta gene complexes, whose proteins contain homeo domain motifs, determines sexual development in the mushroom Coprinus cinereus. Genes Dev. 1992 Apr;6(4):568–577. doi: 10.1101/gad.6.4.568. [DOI] [PubMed] [Google Scholar]
  10. Kües U., Tymon A. M., Richardson W. V., May G., Gieser P. T., Casselton L. A. A mating-type factors of Coprinus cinereus have variable numbers of specificity genes encoding two classes of homeodomain proteins. Mol Gen Genet. 1994 Oct 17;245(1):45–52. doi: 10.1007/BF00279749. [DOI] [PubMed] [Google Scholar]
  11. May G., Le Chevanton L., Pukkila P. J. Molecular analysis of the Coprinus cinereus mating type A factor demonstrates an unexpectedly complex structure. Genetics. 1991 Jul;128(3):529–538. doi: 10.1093/genetics/128.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Shea E. K., Rutkowski R., Kim P. S. Mechanism of specificity in the Fos-Jun oncoprotein heterodimer. Cell. 1992 Feb 21;68(4):699–708. doi: 10.1016/0092-8674(92)90145-3. [DOI] [PubMed] [Google Scholar]
  13. Stankis M. M., Specht C. A., Yang H., Giasson L., Ullrich R. C., Novotny C. P. The A alpha mating locus of Schizophyllum commune encodes two dissimilar multiallelic homeodomain proteins. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7169–7173. doi: 10.1073/pnas.89.15.7169. [DOI] [PMC free article] [PubMed] [Google Scholar]

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