Abstract
In the medfly Ceratitis capitata, the Y chromosome is responsible for determining the male sex. We have mapped the region containing the relevant factor through the analysis of Y-autosome translocations using fluorescence in situ hybridization with two different probes. One probe, the clone pY114, contains repetitive, Y-specific DNA sequences from C. capitata, while the second clone, pDh2-H8, consists of ribosomal DNA sequences from Drosophila hydei. Clone pY114 labeled most of the long arm and pDh2-H8 hybridizes to the short arm and the centromeric region of the long arm. In 12 of the analyzed 19 Y-autosome translocation strains, adjacent-1 segregation products survive to the late pupal or even adult stage and can, therefore, be sexed. This was correlated with the length of the Y fragment still present in these aberrant individuals and allowed us to map the male-determining factor to a region of the long arm representing ~15% of the entire Y chromosome. No additional factors, affecting for example fertility, were detected outside the male-determining region.
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Selected References
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- Anleitner J. E., Haymer D. S. Y enriched and Y specific DNA sequences from the genome of the Mediterranean fruit fly, Ceratitis capitata. Chromosoma. 1992 Mar;101(5-6):271–278. doi: 10.1007/BF00346005. [DOI] [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kraemer C., Schmidt E. R. The sex determining region of Chironomus thummi is associated with highly repetitive DNA and transposable elements. Chromosoma. 1993 Sep;102(8):553–562. doi: 10.1007/BF00368348. [DOI] [PubMed] [Google Scholar]
- Willhoeft U., Traut W. The sex-determining region of the Megaselia scalaris (Diptera) Y chromosome. Chromosome Res. 1995 Jan;3(1):59–65. doi: 10.1007/BF00711163. [DOI] [PubMed] [Google Scholar]
- Zacharopoulou A., Frisardi M., Savakis C., Robinson A. S., Tolias P., Konsolaki M., Komitopoulou K., Kafatos F. C. The genome of the Mediterranean fruitfly Ceratitis capitata: localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes. Chromosoma. 1992 Apr;101(7):448–455. doi: 10.1007/BF00582839. [DOI] [PubMed] [Google Scholar]