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. 1996 Nov;144(3):1205–1214. doi: 10.1093/genetics/144.3.1205

Genetic Mapping of Quantitative Trait Loci Controlling Growth and Wood Quality Traits in Eucalyptus Grandis Using a Maternal Half-Sib Family and Rapd Markers

D Grattapaglia 1, FLG Bertolucci 1, R Penchel 1, R R Sederoff 1
PMCID: PMC1207612  PMID: 8913761

Abstract

Quantitative trait loci (QTL) mapping of forest productivity traits was performed using an open pollinated half-sib family of Eucalyptus grandis. For volume growth, a sequential QTL mapping approach was applied using bulk segregant analysis (BSA), selective genotyping (SG) and cosegregation analysis (CSA). Despite the low heritability of this trait and the heterogeneous genetic background employed for mapping. BSA detected one putative QTL and SG two out of the three later found by CSA. The three putative QTL for volume growth were found to control 13.7% of the phenotypic variation, corresponding to an estimated 43.7% of the genetic variation. For wood specific gravity five QTL were identified controlling 24.7% of the phenotypic variation corresponding to 49% of the genetic variation. Overlapping QTL for CBH, WSG and percentage dry weight of bark were observed. A significant case of digenic epistasis was found, involving unlinked QTL for volume. Our results demonstrate the applicability of the within half-sib design for QTL mapping in forest trees and indicate the existence of major genes involved in the expression of economically important traits related to forest productivity in Eucalyptus grandis. These findings have important implications for marker-assisted tree breeding.

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Selected References

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  1. Arnheim N., Strange C., Erlich H. Use of pooled DNA samples to detect linkage disequilibrium of polymorphic restriction fragments and human disease: studies of the HLA class II loci. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6970–6974. doi: 10.1073/pnas.82.20.6970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beever J. E., George P. D., Fernando R. L., Stormont C. J., Lewin H. A. Associations between genetic markers and growth and carcass traits in a paternal half-sib family of Angus cattle. J Anim Sci. 1990 Feb;68(2):337–344. doi: 10.2527/1990.682337x. [DOI] [PubMed] [Google Scholar]
  3. Bradshaw H. D., Jr, Stettler R. F. Molecular genetics of growth and development in populus. IV. Mapping QTLs with large effects on growth, form, and phenology traits in a forest tree. Genetics. 1995 Feb;139(2):963–973. doi: 10.1093/genetics/139.2.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clamp P. A., Beever J. E., Fernando R. L., McLaren D. G., Schook L. B. Detection of linkage between genetic markers and genes that affect growth and carcass traits in pigs. J Anim Sci. 1992 Sep;70(9):2695–2706. doi: 10.2527/1992.7092695x. [DOI] [PubMed] [Google Scholar]
  5. Grattapaglia D., Sederoff R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics. 1994 Aug;137(4):1121–1137. doi: 10.1093/genetics/137.4.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Groover A., Devey M., Fiddler T., Lee J., Megraw R., Mitchel-Olds T., Sherman B., Vujcic S., Williams C., Neale D. Identification of quantitative trait loci influencing wood specific gravity in an outbred pedigree of loblolly pine. Genetics. 1994 Dec;138(4):1293–1300. doi: 10.1093/genetics/138.4.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haley C. S. Use of DNA fingerprints for the detection of major genes for quantitative traits in domestic species. Anim Genet. 1991;22(3):259–277. doi: 10.1111/j.1365-2052.1991.tb00676.x. [DOI] [PubMed] [Google Scholar]
  8. Kirchgessner M., Reithmayer F., Roth-Maier D. A. Zum Einfluss einer variierten Vitamin-B6-Versorgung auf den Vitamin-B6-Status in Feten und Reproduktionsorganen. Ann Nutr Metab. 1985;29(3):138–146. doi: 10.1159/000176957. [DOI] [PubMed] [Google Scholar]
  9. Lande R., Thompson R. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics. 1990 Mar;124(3):743–756. doi: 10.1093/genetics/124.3.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Michelmore R. W., Paran I., Kesseli R. V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828–9832. doi: 10.1073/pnas.88.21.9828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weller J. I., Kashi Y., Soller M. Power of daughter and granddaughter designs for determining linkage between marker loci and quantitative trait loci in dairy cattle. J Dairy Sci. 1990 Sep;73(9):2525–2537. doi: 10.3168/jds.S0022-0302(90)78938-2. [DOI] [PubMed] [Google Scholar]

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