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. 1996 Nov;144(3):991–1003. doi: 10.1093/genetics/144.3.991

Asm-1(+), a Neurospora Crassa Gene Related to Transcriptional Regulators of Fungal Development

R Aramayo 1, Y Peleg 1, R Addison 1, R Metzenberg 1
PMCID: PMC1207638  PMID: 8913744

Abstract

This report describes the identification, cloning, and molecular analysis of Asm-1(+) (Ascospore maturation 1), the Neurospora crassa homologue of the Aspergillus nidulans stuA (stunted A) gene. The Asm-1(+) gene is constitutively transcribed and encodes an abundant, nucleus-localized 68.5-kD protein. The protein product of Asm-1(+) (ASM-1), contains a potential DNA-binding motif present in related proteins from A. nidulans (StuA), Candida albicans (EFGTF-1), and Saccharomyces cerevisiae (Phd1 and Sok2). This motif is related to the DNA binding motif of the Swi4/Mbp1/Res family of transcription factors that control the cell cycle. Deletion of Asm-1(+) destroys the ability to make protoperithecia (female organs), but does not affect male-specific functions. We propose that the APSES domain (ASM-1, Phd1, StuA, EFGTF-1, and Sok2) defines a group of proteins that constitute a family of related transcription factors involved in the control of fungal development.

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